Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,090,951	C→A	A472A (GCG→GCT)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,090,951	0	C	A	100.0%	201.4 / NA	56	A472A (GCG→GCT)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/28); total (28/28)

GATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAAC  >  NC_000913/4090815‑4091084

gATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCt                                                                                                                                    >  2:407901/1‑140 (MQ=255)

  tttCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCttt                                                                                                                                  <  1:914929/140‑1 (MQ=255)

   ttcattcaCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTg                                                                                                                                 <  1:381184/140‑1 (MQ=255)

   ttcattcaCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTg                                                                                                                                 <  2:119078/140‑1 (MQ=255)

        tcaCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTgcggcg                                                                                                                            <  1:1404244/140‑1 (MQ=255)

         caCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGt                                                                                                                           <  2:140323/140‑1 (MQ=255)

         caCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGt                                                                                                                           <  1:976121/140‑1 (MQ=255)

         caCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGt                                                                                                                           <  1:175012/140‑1 (MQ=255)

                ggTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTAAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACcaca                                                                                                                    <  1:87818/140‑1 (MQ=255)

                  ttCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAAt                                                                                                                  <  2:652614/140‑1 (MQ=255)

                       gCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCttt                                                                                                             <  1:509893/140‑1 (MQ=255)

                       gCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCttt                                                                                                             <  1:570546/140‑1 (MQ=255)

                       gCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCttt                                                                                                             <  1:590657/140‑1 (MQ=255)

                       acGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCttt                                                                                                             <  2:595865/139‑1 (MQ=255)

                            tttATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGa                                                                                                        >  1:231556/1‑140 (MQ=255)

                            tttATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGa                                                                                                        >  2:963420/1‑140 (MQ=255)

                            tttATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGATGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGa                                                                                                        >  2:1139534/1‑140 (MQ=255)

                              tATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACg                                                                                                      <  2:1075961/140‑1 (MQ=255)

                                 cAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATa                                                                                                   <  1:407901/140‑1 (MQ=255)

                                  aGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATaa                                                                                                  >  1:948964/1‑140 (MQ=255)

                                   gAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATaat                                                                                                 <  2:231556/140‑1 (MQ=255)

                                     aTGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCACTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATaatga                                                                                               <  1:179891/140‑1 (MQ=255)

                                      tGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATaatgaa                                                                                              >  1:1406721/1‑140 (MQ=255)

                                         cgcgAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAatgaatga                                                                                           <  2:464007/140‑1 (MQ=255)

                                              aCTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGt                                                                                      >  1:580891/1‑140 (MQ=255)

                                              aCTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGt                                                                                      >  2:697751/1‑140 (MQ=255)

                                                           cgccgcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGcc                                                                         >  2:643166/1‑140 (MQ=255)

                                                            gccgcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCa                                                                        >  1:1263217/1‑140 (MQ=255)

                                                                cTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCg                                                                    >  1:398425/1‑140 (MQ=255)

                                                                 tGACGGCGCTGGCTTCGCACAACTGATGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGc                                                                   <  1:1139534/140‑1 (MQ=255)

                                                                      gcgcTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCa                                                              <  1:697751/140‑1 (MQ=255)

                                                                       cgcTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCAc                                                             <  2:398425/140‑1 (MQ=255)

                                                                          tGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAg                                                          >  1:1372079/1‑140 (MQ=255)

                                                                           ggCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGa                                                         >  2:885522/1‑140 (MQ=255)

                                                                                                 aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACa                                   <  2:416574/140‑1 (MQ=255)

                                                                                                  aCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACa                                   >  2:1472599/1‑139 (MQ=255)

                                                                                                  aCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACa                                   <  1:1472599/139‑1 (MQ=255)

                                                                                                     cAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAg                               <  1:246526/140‑1 (MQ=255)

                                                                                                       aTGTTCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGc                             >  2:476073/1‑140 (MQ=255)

                                                                                                           tCATGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGAt                         <  2:580891/140‑1 (MQ=255)

                                                                                                             aTGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCa                       >  1:639550/1‑140 (MQ=255)

                                                                                                             aTGGACAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCa                       >  1:618050/1‑140 (MQ=255)

                                                                                                              tGGACAAGCTCCTGTTGCGCGGCTTTAGATTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAg                      <  2:1406721/140‑1 (MQ=255)

                                                                                                                  cAAGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCggg                  >  2:942928/1‑140 (MQ=255)

                                                                                                                   aaGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGt                 <  1:942928/140‑1 (MQ=255)

                                                                                                                    aGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTc                >  2:378413/1‑140 (MQ=255)

                                                                                                                    aGCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGGAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTc                >  2:1311297/1‑140 (MQ=255)

                                                                                                                     gCTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCa               >  1:546955/1‑140 (MQ=255)

                                                                                                                      cTCCTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCaa              >  1:1050827/1‑140 (MQ=255)

                                                                                                                         cTGTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGa           >  1:50503/1‑140 (MQ=255)

                                                                                                                           gTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATa         >  1:156135/1‑140 (MQ=255)

                                                                                                                           gTTGCGCGGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATa         >  2:1415321/1‑140 (MQ=255)

                                                                                                                               cgcgGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCa     >  1:1459675/1‑140 (MQ=255)

                                                                                                                               cgcgGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCa     >  2:672298/1‑140 (MQ=255)

                                                                                                                                 cgGCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCaaa   >  1:471459/1‑140 (MQ=255)

                                                                                                                                  ggCTTTAGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAAc  <  2:948964/140‑1 (MQ=255)

GATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAAC  >  NC_000913/4090815‑4091084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A