Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,481,631	C→G	R736P (CGC→CCC)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,481,631	0	C	G	100.0%	228.8 / NA	58	R736P (CGC→CCC)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (28/30); total (28/30)

ACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGC  >  NC_000913/4481497‑4481765

aCGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCATCGTATGGGGAGTa                                                                                                                                   <  2:40033/140‑1 (MQ=255)

aCGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTa                                                                                                                                   >  2:995441/1‑140 (MQ=255)

aCGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTa                                                                                                                                   >  2:570322/1‑140 (MQ=255)

   gCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACcg                                                                                                                                <  2:1397231/140‑1 (MQ=255)

   gCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACcg                                                                                                                                <  1:1441175/140‑1 (MQ=255)

     tGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACcgcg                                                                                                                              <  1:54072/140‑1 (MQ=255)

     tGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACcgcg                                                                                                                              <  1:19135/140‑1 (MQ=255)

     tGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACcgcg                                                                                                                              <  1:960615/140‑1 (MQ=255)

          caTGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGtt                                                                                                                         <  2:1271283/140‑1 (MQ=255)

          aatgatgGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGtt                                                                                                                         <  2:891325/139‑1 (MQ=255)

          aatgatgGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGtt                                                                                                                         <  2:617380/139‑1 (MQ=255)

               tgGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCt                                                                                                                    >  2:123695/1‑140 (MQ=255)

                           tGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCg                                                                                                        >  1:186133/1‑140 (MQ=255)

                                   cAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTAc                                                                                                >  2:850588/1‑140 (MQ=255)

                                          cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTg                                                                                         <  1:1196433/140‑1 (MQ=255)

                                            ttCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGt                                                                                       <  1:36747/140‑1 (MQ=255)

                                             tCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTc                                                                                      >  1:1328170/1‑140 (MQ=255)

                                              cacaCGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCa                                                                                     <  1:1274862/140‑1 (MQ=255)

                                                 acGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCt                                                                                  <  1:850588/140‑1 (MQ=255)

                                                  cGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACTGTTCATTACCGGCTTGGTCAGCTc                                                                                 >  2:402832/1‑140 (MQ=255)

                                                  cGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTc                                                                                 >  1:82269/1‑140 (MQ=255)

                                                                    gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCGCCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGaa                                                               <  1:879001/140‑1 (MQ=255)

                                                                    gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGaa                                                               >  1:51525/1‑140 (MQ=255)

                                                                    gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGaa                                                               >  2:364086/1‑140 (MQ=255)

                                                                    gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGaa                                                               <  2:186133/140‑1 (MQ=255)

                                                                      gatgaACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACt                                                             <  1:1230509/140‑1 (MQ=255)

                                                                       atgaACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGTGCCGCTCGAGTTACCAGTCACAGAACtt                                                            >  2:414869/1‑139 (MQ=255)

                                                                         gaACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGt                                                          <  1:540562/140‑1 (MQ=255)

                                                                               aaTGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAgg                                                    <  1:943462/140‑1 (MQ=255)

                                                                                 tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTg                                                  <  1:213685/140‑1 (MQ=255)

                                                                                 tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTg                                                  <  1:72176/140‑1 (MQ=255)

                                                                                 tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTg                                                  <  1:1227495/140‑1 (MQ=255)

                                                                                 tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTg                                                  <  1:177129/140‑1 (MQ=255)

                                                                                   aTCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGaa                                                <  1:1478121/140‑1 (MQ=255)

                                                                                         tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCGCCGGTCATTACCGGCTTGGTCAGCTCGAGATACCAGTGACAGAACTGGGTGCAGGGGAACGCATa                                          >  1:1412543/1‑140 (MQ=255)

                                                                                         tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTtata                                          >  2:1065901/1‑140 (MQ=255)

                                                                                         tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATa                                          >  1:12741/1‑140 (MQ=255)

                                                                                           cgcgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACa                                        >  2:1406607/1‑140 (MQ=255)

                                                                                             cgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGa                                      <  1:1187419/140‑1 (MQ=255)

                                                                                             cgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGa                                      >  2:1175622/1‑140 (MQ=255)

                                                                                             cgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGa                                      <  1:123695/140‑1 (MQ=255)

                                                                                               aGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAAt                                    <  1:995441/140‑1 (MQ=255)

                                                                                                          aCCTTCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGa                         >  1:575143/1‑140 (MQ=255)

                                                                                                              tCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATc                     <  2:1114218/140‑1 (MQ=255)

                                                                                                              tCCAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATc                     <  1:216207/140‑1 (MQ=255)

                                                                                                               ccAGTACAGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCg                    >  1:779579/1‑140 (MQ=255)

                                                                                                                      aGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTTAGCTGCAGATACCAGTCGCAGAACTAGGTCCAGGGGCACACATACAGAATTGCAGCGCCGAGATCGaaactga             >  2:561684/1‑135 (MQ=255)

                                                                                                                      aGTCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGa             >  2:661513/1‑140 (MQ=255)

                                                                                                                        tCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAg           <  2:779579/140‑1 (MQ=255)

                                                                                                                        tCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAg           >  1:1066546/1‑140 (MQ=255)

                                                                                                                        tCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAg           >  2:307162/1‑140 (MQ=255)

                                                                                                                        tCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAg           >  1:175236/1‑140 (MQ=255)

                                                                                                                        tCACCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAg           >  1:97498/1‑140 (MQ=255)

                                                                                                                          aCCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTTGTCAGCTCTAGATACCAGTCACAGAACTGGTTCCATGGGAACTCATACAGAATGGCTGCGGCTATATCGAAGCGGAAGCt         >  2:537227/1‑140 (MQ=255)

                                                                                                                          aCCAGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCt         >  2:942389/1‑140 (MQ=255)

                                                                                                                             aGCGTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTc      <  2:12741/140‑1 (MQ=255)

                                                                                                                                gTATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAg   >  2:568397/1‑140 (MQ=255)

                                                                                                                                 tATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAgc  >  1:1081810/1‑140 (MQ=255)

                                                                                                                                 tATGGGGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAgc  >  1:1427022/1‑140 (MQ=255)

ACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGC  >  NC_000913/4481497‑4481765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A