Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,043,834 G→T G58V (GGA→GTA)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,043,8340GT100.0% 333.2 / NA 97G58V (GGA→GTA) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/58);  total (39/58)

ATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATG  >  NC_000913/3043700‑3043968
                                                                                                                                      |                                                                                                                                      
aTGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAAt                                                                                                                                   >  1:2134129/1‑140 (MQ=255)
 tGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  <  2:1207729/140‑1 (MQ=255)
 aggggCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  <  2:631907/139‑1 (MQ=255)
  ggggCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATAc                                                                                                                                 <  1:1685949/140‑1 (MQ=255)
   gggCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACt                                                                                                                                >  2:1837168/1‑140 (MQ=255)
   gggCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACt                                                                                                                                >  2:204934/1‑140 (MQ=255)
    ggcggcGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTa                                                                                                                               >  2:2141794/1‑140 (MQ=255)
     gcggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTAt                                                                                                                              >  1:2053751/1‑140 (MQ=255)
      cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  >  1:5202/1‑135 (MQ=255)
      cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  <  2:5202/135‑1 (MQ=255)
      cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATc                                                                                                                             >  1:2026132/1‑140 (MQ=255)
      cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATc                                                                                                                             <  2:445633/140‑1 (MQ=255)
      cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACAC‑GCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATcc                                                                                                                            >  2:92239/1‑140 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           <  2:2134129/140‑1 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           <  1:1368149/140‑1 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           >  1:1698308/1‑140 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           >  2:257201/1‑140 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           >  2:335422/1‑140 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           <  1:2029085/140‑1 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           <  1:204934/140‑1 (MQ=255)
        gcgcAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCa                                                                                                                           >  2:852115/1‑140 (MQ=255)
             gTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCa                                                                                                                      <  1:1185169/140‑1 (MQ=255)
             gTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCa                                                                                                                      <  1:2117084/140‑1 (MQ=255)
              ttGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  <  1:1010588/127‑1 (MQ=255)
              ttGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATa                                                                                                                                  >  2:1010588/1‑127 (MQ=255)
                gCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGa                                                                                                                   <  2:533475/140‑1 (MQ=255)
                gCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGa                                                                                                                   <  2:1611042/140‑1 (MQ=255)
                 ccGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGaa                                                                                                                  <  2:654329/140‑1 (MQ=255)
                         aaGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGa                                                                                                          <  2:137380/139‑1 (MQ=255)
                            gTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGAttt                                                                                                       >  2:1223759/1‑140 (MQ=255)
                              cGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGAttttt                                                                                                     <  1:2083172/140‑1 (MQ=255)
                              cGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGAttttt                                                                                                     <  2:2053751/140‑1 (MQ=255)
                                aaGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTAt                                                                                                   <  1:1666669/140‑1 (MQ=255)
                                 aGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATg                                                                                                  >  2:2068503/1‑140 (MQ=255)
                                  ggcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATgg                                                                                                 <  2:1717102/140‑1 (MQ=255)
                                  ggcggcTGGAACAAAGGCGGAAAAGGACCGAGCATTTGTGACGTTCTGACCGGTGGCGCACAC‑GCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATGCTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:92239/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:706604/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:1837168/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:1759434/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:1847555/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:1090948/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:852115/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:2079860/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:1330416/140‑1 (MQ=255)
                                   gcggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:1178735/140‑1 (MQ=255)
                                   gcagcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  2:1619656/137‑1 (MQ=255)
                                    cggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTc                                                                                               <  2:2070402/140‑1 (MQ=255)
                                     ggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCa                                                                                              <  1:2094581/140‑1 (MQ=255)
                                     ggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCa                                                                                              <  1:2146315/140‑1 (MQ=255)
                                     ggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCa                                                                                              <  2:551540/140‑1 (MQ=255)
                                      gcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                <  1:1713272/137‑1 (MQ=255)
                                      gcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                >  2:1713272/1‑137 (MQ=255)
                                       cTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCATTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACt                                                                                            <  1:613905/140‑1 (MQ=255)
                                       cTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACt                                                                                            <  1:335422/140‑1 (MQ=255)
                                         ggAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtat                                                                                          <  1:257201/140‑1 (MQ=255)
                                         ggAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtag                                                                                          <  2:2026132/140‑2 (MQ=255)
                                         ggAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACC‑GTGGCGCACACGGCGTGCCGCGGGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGACGTTGATTTTTATGGTCACtata                                                                                         <  2:1994387/140‑1 (MQ=255)
                                          gAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                         <  2:685579/140‑1 (MQ=255)
                                          gAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                         >  1:785377/1‑140 (MQ=255)
                                          gAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                         <  2:608133/140‑1 (MQ=255)
                                              aaaGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGa                                                                                     <  2:827208/140‑1 (MQ=255)
                                               aaGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGaa                                                                                    <  2:281431/140‑1 (MQ=255)
                                                    ggAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACAt                                                                               >  2:1482696/1‑140 (MQ=255)
                                                     gAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATc                                                                              >  2:2007349/1‑140 (MQ=255)
                                                     gAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACAAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATc                                                                              >  1:900144/1‑140 (MQ=255)
                                                             ccGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTAtt                                                                      >  2:329357/1‑140 (MQ=255)
                                                                aGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGc                                                                   >  1:1401733/1‑140 (MQ=255)
                                                                     ttGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGaaa                                                              <  2:1698308/140‑1 (MQ=255)
                                                                     ttGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGaaa                                                              <  1:329357/140‑1 (MQ=255)
                                                                      tgtgACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAAt                                                             >  1:1024431/1‑140 (MQ=255)
                                                                       gtgACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATg                                                            <  1:361800/140‑1 (MQ=255)
                                                                       gtgACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATg                                                            <  2:269461/140‑1 (MQ=255)
                                                                             ttCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTc                                                      >  2:1304847/1‑140 (MQ=255)
                                                                             ttCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTc                                                      >  1:234471/1‑140 (MQ=255)
                                                                               cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAGTACATCAAGCTATTTGCCGAAATGGGCTTCaa                                                    >  2:675476/1‑140 (MQ=255)
                                                                               cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCaa                                                    >  1:258886/1‑140 (MQ=255)
                                                                               cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCaa                                                    >  2:897586/1‑140 (MQ=255)
                                                                                tGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCaaa                                                   >  1:1616521/1‑140 (MQ=255)
                                                                                tGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCaaa                                                   <  2:1401733/140‑1 (MQ=255)
                                                                                      gTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt                                             >  2:1661243/1‑140 (MQ=255)
                                                                                          cgcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCATTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa                                         <  2:1147482/140‑1 (MQ=255)
                                                                                          cgcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa                                         <  1:1661243/140‑1 (MQ=255)
                                                                                          cgcACACGGCGTGCCGCGCGAAATCACAAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa                                         <  2:900144/140‑1 (MQ=255)
                                                                                              cacGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc                                     <  1:2007349/140‑1 (MQ=255)
                                                                                                cGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa                                   <  1:1064989/140‑1 (MQ=255)
                                                                                                aggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa                                   <  2:850495/139‑1 (MQ=255)
                                                                                                 ggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc                                     <  1:137985/137‑1 (MQ=255)
                                                                                                 ggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc                                     >  2:137985/1‑137 (MQ=255)
                                                                                                 ggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt                                  >  1:1355555/1‑140 (MQ=255)
                                                                                                 ggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt                                  >  2:1388386/1‑140 (MQ=255)
                                                                                                    gTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGc                               >  1:420861/1‑140 (MQ=255)
                                                                                                               aaTCACCAAAGAAGTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCa                    >  1:1666229/1‑140 (MQ=255)
                                                                                                                       aagaagTCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCa            <  2:1666229/140‑1 (MQ=255)
                                                                                                                             tCTTGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGc      >  1:1735518/1‑140 (MQ=255)
                                                                                                                               ttGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCgt    >  1:681111/1‑139 (MQ=255)
                                                                                                                               ttGCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa    >  1:1362784/1‑140 (MQ=255)
                                                                                                                                 gCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAATACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg  >  2:12892/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATG  >  NC_000913/3043700‑3043968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A