Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,430	C→A	E251D (GAG→GAT)	yggF ←	fructose 1,6‑bisphosphatase YggF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,430	0	C	A	100.0%	428.5 / NA	124	E251D (GAG→GAT)	yggF	fructose 1,6‑bisphosphatase YggF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (54/70); total (54/70)

Only 100 of 131 total aligned reads displayed.

GCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATG  >  NC_000913/3075293‑3075565

gCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATc                                                                                                                                       <  1:324670/140‑1 (MQ=255)

accGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATc                                                                                                                                       <  2:879943/139‑1 (MQ=255)

  cGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCt                                                                                                                                     <  1:970428/140‑1 (MQ=255)

                cGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGc                                                                                                                       >  2:1402099/1‑140 (MQ=255)

                    cACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGAt                                                                                                                   <  2:797899/140‑1 (MQ=255)

                       cAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGAtttt                                                                                                                <  1:2050026/140‑1 (MQ=255)

                       cAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGAtttt                                                                                                                <  1:775387/140‑1 (MQ=255)

                          cTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCg                                                                                                             >  1:2011344/1‑140 (MQ=255)

                          cTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCg                                                                                                             >  1:782631/1‑140 (MQ=255)

                            cACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCgtg                                                                                                           <  2:711864/140‑1 (MQ=255)

                              ccGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTa                                                                                                         <  1:1727271/140‑1 (MQ=255)

                              acGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTa                                                                                                         <  2:950414/139‑1 (MQ=255)

                               cGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTaa                                                                                                        <  1:1773729/140‑1 (MQ=255)

                               cGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTaa                                                                                                        >  1:1759127/1‑140 (MQ=255)

                                gCCCGTGACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCACCGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAAt                                                                                                       <  2:1104186/140‑1 (MQ=255)

                                gCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAAt                                                                                                       <  2:1632266/140‑1 (MQ=255)

                                gCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAAt                                                                                                       <  2:2105228/140‑1 (MQ=255)

                                gCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAAt                                                                                                       <  2:1567633/140‑1 (MQ=255)

                                gCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAAt                                                                                                       <  1:951020/140‑1 (MQ=255)

                                    gTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATcccc                                                                                                   >  1:729730/1‑140 (MQ=255)

                                    gTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATcccc                                                                                                   >  1:1494302/1‑140 (MQ=255)

                                          cccGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGc                                                                                             <  2:1494302/140‑1 (MQ=255)

                                          cccGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGc                                                                                             <  2:1459121/140‑1 (MQ=255)

                                           ccGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGcc                                                                                            <  2:2114126/140‑1 (MQ=255)

                                              tAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTgg                                                                                         >  2:586411/1‑140 (MQ=255)

                                              tAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTgg                                                                                         >  1:1883925/1‑140 (MQ=255)

                                               aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGc                                                                                        <  2:477032/140‑1 (MQ=255)

                                               aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGc                                                                                        <  1:452766/140‑1 (MQ=255)

                                               aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGc                                                                                        <  1:678099/140‑1 (MQ=255)

                                               aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGc                                                                                        <  1:1754650/140‑1 (MQ=255)

                                               aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGc                                                                                        <  1:1115920/140‑1 (MQ=255)

                                                   cTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCaaaa                                                                                    >  1:2014618/1‑140 (MQ=255)

                                                     aaaGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGt                                                                                  >  1:431103/1‑140 (MQ=255)

                                                      aaGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTc                                                                                 >  1:1555692/1‑140 (MQ=255)

                                                           gATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCa                                                                            >  2:1170299/1‑140 (MQ=255)

                                                           gATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCa                                                                            >  2:1302844/1‑140 (MQ=255)

                                                           gATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCa                                                                            >  1:520431/1‑140 (MQ=255)

                                                             tttCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGc                                                                          <  1:53025/138‑1 (MQ=255)

                                                             tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGc                                                                          <  2:1281866/140‑1 (MQ=255)

                                                             tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGc                                                                          <  2:1299747/140‑1 (MQ=255)

                                                              atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt                                                                         >  2:1984438/1‑140 (MQ=255)

                                                              atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt                                                                         <  2:76869/140‑1 (MQ=255)

                                                              atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt                                                                         <  2:697327/140‑1 (MQ=255)

                                                              atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt                                                                         <  2:1759127/140‑1 (MQ=255)

                                                                cGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCg                                                                       <  2:771175/140‑1 (MQ=255)

                                                                cGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCg                                                                       <  2:1601786/140‑1 (MQ=255)

                                                                  ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAAtcggctcggc                                                                     <  1:198094/140‑1 (MQ=255)

                                                                  ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGc                                                                     <  2:2011344/140‑1 (MQ=255)

                                                                  ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGc                                                                     <  2:579572/140‑1 (MQ=255)

                                                                   tCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGcc                                                                    >  2:1344137/1‑140 (MQ=255)

                                                                          cACCAGTTCATCGAGCGAGTACACACGGTTAACGCCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCatat                                                             >  1:1136428/1‑140 (MQ=255)

                                                                           aCCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATc                                                            >  2:289949/1‑140 (MQ=255)

                                                                           aCCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATc                                                            >  2:17608/1‑140 (MQ=255)

                                                                            ccAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATcc                                                           >  2:788146/1‑140 (MQ=255)

                                                                               gTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCg                                                        >  2:2003691/1‑140 (MQ=255)

                                                                               gTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTAGGCCTGCATATCCCCg                                                        >  2:1704514/1‑140 (MQ=255)

                                                                                 tCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGcc                                                      <  2:1706901/140‑1 (MQ=255)

                                                                                 tCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGcc                                                      >  1:1070571/1‑140 (MQ=255)

                                                                                  cATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCt                                                     <  1:769288/140‑1 (MQ=255)

                                                                                  cATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCt                                                     <  2:737881/140‑1 (MQ=255)

                                                                                   aTCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTa                                                    <  1:1321886/140‑1 (MQ=255)

                                                                                       agcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTa                                                    >  2:1400533/1‑136 (MQ=255)

                                                                                       agcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTa                                                    <  1:1400533/136‑1 (MQ=255)

                                                                                       agcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATgt                                                >  2:621530/1‑139 (MQ=255)

                                                                                         cgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCtt                                              >  2:1944262/1‑140 (MQ=255)

                                                                                             tACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAAc                                          <  1:1170299/140‑1 (MQ=255)

                                                                                                 cacGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGca                                      <  1:1455523/140‑1 (MQ=255)

                                                                                                 aaccgTTAACGTCAACGCCCATTGCCTTGCACCTCTTGCGATCCTGCTCTGCAATCTACCGATTTCCCGGGTAATCCCCTTTAGCGTGGCAAAAGTCAATCCGCTCGGCCTGCATCTCCCCGCCTAATGCTTTAACGGca                                      <  2:124296/136‑1 (MQ=255)

                                                                                                  acGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGcac                                     >  1:912488/1‑140 (MQ=255)

                                                                                                     gTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAgg                                  <  1:1944262/140‑1 (MQ=255)

                                                                                                      ttAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGc                                 >  1:1649261/1‑140 (MQ=255)

                                                                                                      ttAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATAT‑CCCGCCTAATGCTTTAACGGCACAGGCa                                >  1:502692/1‑140 (MQ=255)

                                                                                                       tAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCa                                >  2:282671/1‑140 (MQ=255)

                                                                                                       tAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCa                                <  1:991920/140‑1 (MQ=255)

                                                                                                       tAACGTCAACGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCa                                >  2:1603812/1‑140 (MQ=255)

                                                                                                              aaCGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCa                         >  2:1353092/1‑140 (MQ=255)

                                                                                                              aaCGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCa                         >  2:2068554/1‑140 (MQ=255)

                                                                                                              aaCGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGAATTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCa                         >  1:660389/1‑140 (MQ=255)

                                                                                                                cGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACg                       <  1:596941/140‑1 (MQ=255)

                                                                                                                cGCCCATTGCCTTGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACg                       >  1:996683/1‑140 (MQ=255)

                                                                                                                           ttGCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGgcgc            >  2:1864990/1‑140 (MQ=255)

                                                                                                                             gaaccGCTTGCGATCTTGCTCTGCAATCTGCCGTTTTTCCGTTTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCCCGCCTTCTGGCGCAc          <  2:651443/136‑1 (MQ=255)

                                                                                                                             gCAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAc          >  1:1468089/1‑140 (MQ=255)

                                                                                                                              cAACGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAcc         <  1:1302844/140‑1 (MQ=255)

                                                                                                                                aCGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgc       <  2:8536/140‑1 (MQ=255)

                                                                                                                                aCGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTAAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgc       <  2:1468089/140‑1 (MQ=255)

                                                                                                                                 cGCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgcc      >  1:272269/1‑140 (MQ=255)

                                                                                                                                  gCTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgccg     <  1:863736/140‑1 (MQ=255)

                                                                                                                                   cTTGGGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  1:449986/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGTTTTTCCGTGTAATCCCCTTTAGCCGGGCAAAAGTCAATCAGCGCGGCCTGAATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  2:926466/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  1:1102599/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    >  1:1247908/1‑140 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  1:1353092/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    >  1:1665301/1‑140 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  1:1814376/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    >  1:1929413/1‑140 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  1:2003691/140‑1 (MQ=255)

                                                                                                                                   cTTGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa    <  2:359718/140‑1 (MQ=255)

                                                                                                                                     tGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATg  <  1:1652196/140‑1 (MQ=255)

                                                                                                                                     tGCGATCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATg  <  1:289949/140‑1 (MQ=255)

GCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATG  >  NC_000913/3075293‑3075565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A