Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,796	C→A	A129A (GCG→GCT)	yggF ←	fructose 1,6‑bisphosphatase YggF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,796	0	C	A	97.7%	281.2 / ‑3.2	88	A129A (GCG→GCT)	yggF	fructose 1,6‑bisphosphatase YggF
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (37/49); total (38/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GTGTTGCTTCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCCGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAATGGGAT  >  NC_000913/3075660‑3075932

gtgtTGCTTCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGcgccagcc                                                                                                                                       >  1:287552/1‑140 (MQ=255)

  gtTGCTTCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCagccgc                                                                                                                                     <  1:895329/140‑1 (MQ=255)

     gCTTCCTCAAGCGCGGCACTCAGGCGCGGTTTATCCAGCGTAGCCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCGGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAg                                                                                                                                  <  1:537885/140‑1 (MQ=255)

      cTTCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGcgccagccgccagc                                                                                                                                 <  1:712925/140‑1 (MQ=255)

        tCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGccagccgccagccg                                                                                                                               >  1:1762784/1‑140 (MQ=255)

        tCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGccagccgccagccg                                                                                                                               >  1:1251907/1‑140 (MQ=255)

           tCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGAtt                                                                                                                            >  1:1977159/1‑140 (MQ=255)

           tCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGAtt                                                                                                                            >  2:2131728/1‑140 (MQ=255)

           tCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGAtt                                                                                                                            >  2:442532/1‑140 (MQ=255)

           tCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGAtt                                                                                                                            >  1:1505191/1‑140 (MQ=255)

                 gcgGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTaacaac                                                                                                                      <  1:1938897/140‑1 (MQ=255)

                  cgGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAAcc                                                                                                                     <  2:1954136/140‑1 (MQ=255)

                    gCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCCGCCGCCAGCCGATTAACAACCat                                                                                                                   >  2:1161152/1‑139 (MQ=255)

                    gCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAg                                                                                                                   >  1:747043/1‑140 (MQ=255)

                      aCTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCGCGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCt                                                                                                                 <  2:1935893/140‑1 (MQ=255)

                      aCTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCt                                                                                                                 <  2:813387/140‑1 (MQ=255)

                      aCTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCt                                                                                                                 <  1:592355/140‑1 (MQ=255)

                      aCTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCt                                                                                                                 <  2:287552/140‑1 (MQ=255)

                      aCTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCt                                                                                                                 <  1:1957015/140‑1 (MQ=255)

                       cTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCtt                                                                                                                <  1:1553429/140‑1 (MQ=255)

                       cTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCtt                                                                                                                <  1:452238/140‑1 (MQ=255)

                        tCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttt                                                                                                               <  1:546865/140‑1 (MQ=255)

                        tCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttt                                                                                                               <  1:1850126/140‑1 (MQ=255)

                        tCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttt                                                                                                               <  2:816402/140‑1 (MQ=255)

                          aGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttttt                                                                                                             <  2:1251907/140‑1 (MQ=255)

                          aGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttttt                                                                                                             <  1:1567705/140‑1 (MQ=255)

                          aGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttttt                                                                                                             <  2:1505191/140‑1 (MQ=255)

                          aGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCttttt                                                                                                             <  1:1363336/140‑1 (MQ=255)

                           ggCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTc                                                                                                            <  2:632250/140‑1 (MQ=255)

                           ggCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTc                                                                                                            <  1:2131728/140‑1 (MQ=255)

                           ggCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTc                                                                                                            <  2:371184/140‑1 (MQ=255)

                            gcgcGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCa                                                                                                           <  1:228224/140‑1 (MQ=255)

                              gcgGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCata                                                                                                         <  1:1578983/140‑1 (MQ=255)

                               cgGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCTCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCatat                                                                                                        >  2:1435789/1‑140 (MQ=255)

                               cgGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCatat                                                                                                        >  1:333486/1‑140 (MQ=255)

                                 gTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATAc                                                                                                      >  1:1257302/1‑140 (MQ=255)

                                        cAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCCGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCgg                                                                                               <  1:1161152/140‑1 (MQ=255)

                                        cAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCgg                                                                                               <  1:1435789/140‑1 (MQ=255)

                                        aaGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTACGGAGATTGTCCGTCAGAGGCAAAGACAGATCAATCGTGTTAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATTCATATCga                                                                                               <  2:170520/139‑2 (MQ=255)

                                         agagTAGCCAGGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGATGTCCGTCAGAGGCCAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCggg                                                                                              <  2:1790136/137‑1 (MQ=255)

                                          gCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgc                                                                                             <  1:1052290/140‑1 (MQ=255)

                                          gCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgc                                                                                             >  1:1868304/1‑140 (MQ=255)

                                          gCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGATTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgc                                                                                             <  2:1905740/140‑1 (MQ=255)

                                            gTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcgc                                                                                           >  1:1367951/1‑140 (MQ=255)

                                            gTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcgc                                                                                           <  2:1977159/140‑1 (MQ=255)

                                            gTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcgc                                                                                           >  2:1897105/1‑140 (MQ=255)

                                            gTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcgc                                                                                           >  1:740161/1‑140 (MQ=255)

                                                 cATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTa                                                                                      <  2:1868304/140‑1 (MQ=255)

                                                 cATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTa                                                                                      <  1:2012014/140‑1 (MQ=255)

                                                     cgcAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAg                                                                                  >  2:789191/1‑140 (MQ=255)

                                                     cgcAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAg                                                                                  >  2:1986702/1‑140 (MQ=255)

                                                     cgcAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAg                                                                                  >  1:751373/1‑140 (MQ=255)

                                                      gcAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAgg                                                                                 <  2:1736274/140‑1 (MQ=255)

                                                      gcAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAgg                                                                                 <  2:766442/140‑1 (MQ=255)

                                                        aGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCt                                                                               <  1:1986702/140‑1 (MQ=255)

                                                          cTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcg                                                                                            >  2:522838/1‑125 (MQ=255)

                                                          cTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGgcg                                                                                            <  1:522838/125‑1 (MQ=255)

                                                          cTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTAt                                                                             >  2:290870/1‑140 (MQ=255)

                                                           ttATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTAtc                                                                            >  1:1743436/1‑140 (MQ=255)

                                                                    ggTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCTGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGc                                                                   >  1:1709561/1‑140 (MQ=255)

                                                                     gTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCg                                                                  <  2:1762784/140‑1 (MQ=255)

                                                                        tGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGaaa                                                               >  2:2105442/1‑140 (MQ=255)

                                                                                   ttCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGc                                                    <  1:41733/140‑1 (MQ=255)

                                                                                    tCGCCACGTTGCGCAGGTTGTCCGTCATAGGCAAATACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGcc                                                   >  1:1069129/1‑140 (MQ=255)

                                                                                    tCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGAcc                                                      <  1:307424/137‑1 (MQ=255)

                                                                                    tCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGAcc                                                      >  2:307424/1‑137 (MQ=255)

                                                                                    tCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGcc                                                   >  2:881290/1‑140 (MQ=255)

                                                                                          ggTTGCGCGGGTTGTCGGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCAAGCCGATAAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCa                                             <  2:722998/139‑1 (MQ=255)

                                                                                          cGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCa                                             >  2:444350/1‑140 (MQ=255)

                                                                                          cGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCa                                             <  2:333486/140‑1 (MQ=255)

                                                                                          cGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCa                                             <  1:881290/140‑1 (MQ=255)

                                                                                          atttGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATAAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCa                                             <  2:747043/138‑1 (MQ=255)

                                                                                               cgcAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGct                                        >  2:1562168/1‑140 (MQ=255)

                                                                                               cgcAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGct                                        >  1:785953/1‑140 (MQ=255)

                                                                                                     ttGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGccc                                  >  2:1532915/1‑140 (MQ=255)

                                                                                                       gTCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGcccct                                >  2:277008/1‑139 (MQ=255)

                                                                                                        tCCGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCAt                               <  2:785953/140‑1 (MQ=255)

                                                                                                         ccGTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATc                              >  1:252713/1‑140 (MQ=255)

                                                                                                           gTCAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGc                            <  1:789191/140‑1 (MQ=255)

                                                                                                             cAGAGGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGcca                          <  2:1743436/140‑1 (MQ=255)

                                                                                                              agagGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGccac                         >  1:199395/1‑140 (MQ=255)

                                                                                                               gagGCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGccacc                        >  1:1456605/1‑140 (MQ=255)

                                                                                                                  gCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATc                     >  2:1956130/1‑140 (MQ=255)

                                                                                                                  gCAAAGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATc                     >  2:156337/1‑140 (MQ=255)

                                                                                                                    aaaGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCcg                   >  1:1020220/1‑140 (MQ=255)

                                                                                                                     aaGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCcgc                  <  1:444974/140‑1 (MQ=255)

                                                                                                                      aGACAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCcgcg                 <  1:355427/140‑1 (MQ=255)

                                                                                                                        aCAGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTg               <  2:1474468/140‑1 (MQ=255)

                                                                                                                          aGATCAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGcc             <  1:290870/140‑1 (MQ=255)

                                                                                                                            agcAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCtt           >  1:1670774/3‑140 (MQ=255)

                                                                                                                              cAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCa         <  1:277008/140‑1 (MQ=255)

                                                                                                                              cAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCa         <  1:1688711/140‑1 (MQ=255)

                                                                                                                              cAATCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCa         <  2:1367951/140‑1 (MQ=255)

                                                                                                                              aaaTCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCa         <  2:740161/139‑1 (MQ=255)

                                                                                                                              aaaTCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTCAATATCCATAACGGGCGAATGTAACAGGCTTTCTCGAGGGGGGAAAACCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCa         <  2:751373/139‑1 (MQ=255)

                                                                                                                                aTCGCGCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAAt       <  1:1562168/140‑1 (MQ=255)

                                                                                                                                  cgcgCCAGCCGCCATCCGTTTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATCACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAATgc     >  2:584688/1‑139 (MQ=255)

                                                                                                                                  cgcgCCAGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAATgg     >  2:1349751/1‑140 (MQ=255)

                                                                                                                                     gccagccgccagccGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAATGGGAt  >  1:562651/1‑140 (MQ=255)

GTGTTGCTTCCTCAATCGCGGCACTCAGGCGCGGTTTATCCAGCGTAACCATGCGCAGCTTATCCAGCGGTTTGCCTAACGCTTTCGCCACGTTGCGCAGGTTGTCCGTCAGAGGCAAAGACAGATCAATCGCGCCCGCCGCCAGCCGATTAACAACCAGCTTTTTCATATACATATCGGGCGCATGTAACAGGCTATCTCGAGGGGCGAAAGCCATGACCGCCAGCGCATTGCTCTGCCCCATCGCCACCATCCGCGTGCCTTCAATGGGAT  >  NC_000913/3075660‑3075932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A