Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,299	G→T	Q503K (CAA→AAA)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,299	0	G	T	100.0%	419.2 / NA	120	Q503K (CAA→AAA)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (52/68); total (52/68)

Only 100 of 123 total aligned reads displayed.

GTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCC  >  NC_000913/3122162‑3122434

         cgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgc                                                                                                                              <  2:1580837/140‑1 (MQ=255)

         cgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgc                                                                                                                              >  2:1507417/1‑140 (MQ=255)

         cgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgc                                                                                                                              <  2:1254241/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  1:863688/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  1:339193/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  2:212083/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  2:52868/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  1:924791/140‑1 (MQ=255)

          gcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACcgcg                                                                                                                             <  1:1148101/140‑1 (MQ=255)

           cATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCgcgc                                                                                                                            >  2:449874/1‑140 (MQ=255)

           cATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCgcgc                                                                                                                            >  1:2083367/1‑140 (MQ=255)

           cATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCgcgc                                                                                                                            >  2:657413/1‑140 (MQ=255)

           cATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCgcgc                                                                                                                            >  1:993453/1‑140 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  1:885716/140‑1 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  2:1852637/140‑1 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  1:643371/140‑1 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  1:1226047/140‑1 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  2:431844/140‑1 (MQ=255)

            aTGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCa                                                                                                                           <  1:1887103/140‑1 (MQ=255)

             tGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAg                                                                                                                          >  1:840821/1‑140 (MQ=255)

              ggAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGc                                                                                                                         >  1:289362/1‑140 (MQ=255)

                 gCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCca                                                                                                                      <  1:449874/140‑1 (MQ=255)

                    tAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacag                                                                                                                   >  2:1354617/1‑140 (MQ=255)

                     agcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacaga                                                                                                                  >  1:143016/1‑140 (MQ=255)

                     agcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacaga                                                                                                                  >  2:1689319/1‑140 (MQ=255)

                      gcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCTCacagaa                                                                                                                 <  1:1198837/140‑1 (MQ=255)

                      gcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacagaa                                                                                                                 <  2:853134/140‑1 (MQ=255)

                      gcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacagaa                                                                                                                 >  2:1552365/1‑140 (MQ=255)

                      acagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacagaa                                                                                                                 <  2:627305/139‑1 (MQ=255)

                          cGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacagaacaga                                                                                                             >  1:682510/1‑140 (MQ=255)

                          cGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCacagaacaga                                                                                                             >  1:730883/1‑140 (MQ=255)

                             ttGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCg                                                                                                          <  1:838797/140‑1 (MQ=255)

                               ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGa                                                                                                        <  2:1503231/140‑1 (MQ=255)

                               ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGa                                                                                                        <  2:1043221/140‑1 (MQ=255)

                               ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGa                                                                                                        <  2:321283/140‑1 (MQ=255)

                               ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGa                                                                                                        <  1:1552365/140‑1 (MQ=255)

                                gTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGaa                                                                                                       <  1:1354617/140‑1 (MQ=255)

                                 tGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGaaa                                                                                                      <  1:1835862/140‑1 (MQ=255)

                                  gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACGGACCGGAAAg                                                                                                     <  1:1896059/140‑1 (MQ=255)

                                  gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAg                                                                                                     <  1:611038/140‑1 (MQ=255)

                                  gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTAACAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAg                                                                                                     <  2:2083367/140‑1 (MQ=255)

                                    cGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCa                                                                                                   <  1:1507417/140‑1 (MQ=255)

                                    cGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCa                                                                                                   <  1:163896/140‑1 (MQ=255)

                                          ccAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGttat                                                                                             <  1:1689319/140‑1 (MQ=255)

                                            aGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGttatta                                                                                           <  2:1093572/140‑1 (MQ=255)

                                             ggCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTA‑CAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACg                                                                                         <  1:261363/140‑1 (MQ=255)

                                              gCTGTGTTTGCCCCGGCACGAAGTTGGCCGACCTTCTGGCTGTACATGTCTGACATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGACCAGACCGGAAAGCACGTTATTACg                                                                                         <  2:2024424/140‑1 (MQ=255)

                                              gCTGTGTTTGCCACGGCACGCAGTTGGTCGCCCTTATGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGCCCGGAAAGCACGTTATTACg                                                                                         <  1:1889666/140‑1 (MQ=255)

                                                   gtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGt                                                                                    >  1:72059/1‑140 (MQ=255)

                                                   gtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGt                                                                                    <  2:682510/140‑1 (MQ=255)

                                                        gcccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCt                                                                               <  2:1626770/139‑1 (MQ=255)

                                                        ccccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCt                                                                               <  2:557190/140‑1 (MQ=255)

                                                        ccccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCt                                                                               <  1:1609553/140‑1 (MQ=255)

                                                        ccccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCt                                                                               >  2:314202/1‑140 (MQ=255)

                                                        ccccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCt                                                                               <  2:840821/140‑1 (MQ=255)

                                                            ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGa                                                                           >  2:1932620/1‑140 (MQ=255)

                                                            ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGa                                                                           >  1:473750/1‑140 (MQ=255)

                                                            ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGa                                                                           >  1:1229091/1‑140 (MQ=255)

                                                               aCGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATcc                                                                        <  2:1229091/140‑1 (MQ=255)

                                                                          cGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGAt                                                             >  1:1394215/1‑140 (MQ=255)

                                                                             ccTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTc                                                          >  2:656022/1‑140 (MQ=255)

                                                                               ttCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCAt                                                        >  1:1176691/1‑140 (MQ=255)

                                                                               ttCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCAt                                                        >  1:942677/1‑140 (MQ=255)

                                                                                 cTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCt                                                      >  2:9913/1‑140 (MQ=255)

                                                                                       gTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAtttt                                                >  2:874805/1‑140 (MQ=255)

                                                                                         aCATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                                              >  2:2093702/1‑140 (MQ=255)

                                                                                            tGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgc                                           <  2:289362/140‑1 (MQ=255)

                                                                                              tCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgcag                                         >  1:1258910/1‑140 (MQ=255)

                                                                                              tCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgcag                                         >  1:1871900/1‑140 (MQ=255)

                                                                                              tCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACg                                            <  2:363243/137‑1 (MQ=255)

                                                                                              tCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACg                                            >  1:363243/1‑137 (MQ=255)

                                                                                                tGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgcagct                                       >  2:46099/1‑139 (MQ=255)

                                                                                                  ccATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACg                                            >  2:948672/1‑133 (MQ=255)

                                                                                                  ccATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACg                                            <  1:948672/133‑1 (MQ=255)

                                                                                                  ccATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATc                                     >  1:142435/1‑140 (MQ=255)

                                                                                                  ccATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATc                                     >  2:294703/1‑140 (MQ=255)

                                                                                                  ccATCAGGTCAGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATc                                     >  2:1664898/1‑140 (MQ=255)

                                                                                                    aTCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCgg                                   >  1:135136/1‑140 (MQ=255)

                                                                                                    aTCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCgg                                   >  2:840970/1‑140 (MQ=255)

                                                                                                    aTCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCACCgg                                   >  1:1326978/1‑140 (MQ=255)

                                                                                                     tCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCggg                                  <  1:2025762/140‑1 (MQ=255)

                                                                                                        ggTCCGGCATTGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCa                               <  1:656022/140‑1 (MQ=255)

                                                                                                                  tGCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCa                     <  1:1313661/140‑1 (MQ=255)

                                                                                                                   gCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCAc                    >  2:1119227/1‑140 (MQ=255)

                                                                                                                   gCCCACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCAc                    >  1:1705096/1‑140 (MQ=255)

                                                                                                                   gCCCACATGCCATTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCAc                    >  2:1693418/1‑140 (MQ=255)

                                                                                                                    cccACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCAcc                   >  2:1881544/1‑140 (MQ=255)

                                                                                                                    cccACATGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCCGATTTTTACGCAGCATCGGGCCAGCTTCCATCAcc                   <  2:730883/140‑1 (MQ=255)

                                                                                                                         aTGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATg              >  1:1942524/1‑140 (MQ=255)

                                                                                                                         aTGCCTTTACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATg              >  1:437489/1‑140 (MQ=255)

                                                                                                                              tttACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCGAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCAttt         <  1:1751685/140‑1 (MQ=255)

                                                                                                                              tttACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCAttt         >  1:487945/1‑140 (MQ=255)

                                                                                                                              tttACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCAttt         >  1:1703997/1‑140 (MQ=255)

                                                                                                                              tttACCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCAttt         >  1:1935472/1‑140 (MQ=255)

                                                                                                                               ttACCAATTTTCGCTTTCCCGCGCAGCAGACAGAACAGAGTGGAAAGCACGTTATTACGCTCGTAGGGTTTGATCCAAGGCATCGATTTAATATGATTTTTACGCAGCATCGGGCCAGCTTCCATCTCCGAATGCATTTc        <  2:72059/140‑1 (MQ=255)

                                                                                                                                 aCCAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCAt      <  2:143016/140‑1 (MQ=255)

                                                                                                                                  ccAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATc     <  2:601864/140‑1 (MQ=255)

                                                                                                                                  acAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATc     <  2:1258910/139‑1 (MQ=255)

                                                                                                                                   cAATTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCg    >  1:1271745/1‑140 (MQ=255)

                                                                                                                                     aTTTTCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGcc  >  1:508530/1‑140 (MQ=255)

GTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCC  >  NC_000913/3122162‑3122434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A