Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,150,149 C→A A163E (GCG→GAG)  yghA → NADP(+)‑dependent aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,150,1490CA98.8% 273.0 / ‑2.7 82A163E (GCG→GAG) yghANADP(+)‑dependent aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (36/45);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

CGCTGGTTCACGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAA  >  NC_000913/3150011‑3150284
                                                                                                                                          |                                                                                                                                       
cGCTGGTTCACGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACATTTTcgcg                                                                                                                                        <  2:1306302/140‑1 (MQ=255)
     gTTCACGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGtt                                                                                                                                   <  1:357514/140‑1 (MQ=255)
        cACGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCtt                                                                                                                                >  2:184216/1‑139 (MQ=255)
         aCGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAGCGTTTTCGAGCTGTTCTgg                                                                                                                               <  2:1579292/140‑1 (MQ=255)
               cgcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAAcc                                                                                                                         >  1:838899/1‑140 (MQ=255)
                gcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAAccc                                                                                                                        <  2:343866/140‑1 (MQ=255)
                 cacaAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCa                                                                                                                       >  1:1234226/1‑140 (MQ=255)
                  acaAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAg                                                                                                                      <  2:691523/140‑1 (MQ=255)
                   caAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTCGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAgg                                                                                                                     <  1:1257393/140‑1 (MQ=255)
                         gTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGa                                                                                                               >  1:52575/1‑140 (MQ=255)
                          ttAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGTGAt                                                                                                              <  2:767515/140‑1 (MQ=255)
                          ttAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGAt                                                                                                              <  2:2125925/140‑1 (MQ=255)
                              gCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                                                                                          >  1:1908041/1‑140 (MQ=255)
                              gCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                                                                                          <  2:838899/140‑1 (MQ=255)
                              gCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                                                                                          <  1:1690719/140‑1 (MQ=255)
                                 ggCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTg                                                                                                       <  2:1610719/140‑1 (MQ=255)
                                  gCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc                                                                                                      >  1:16291/1‑140 (MQ=255)
                                        aattatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGa                                                                                                <  2:1234226/139‑1 (MQ=255)
                                         attatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaa                                                                                               <  1:1681816/140‑1 (MQ=255)
                                            atGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                                                                                            >  1:1091655/1‑140 (MQ=255)
                                              ggCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                                                                                          <  1:1940479/124‑1 (MQ=255)
                                              ggCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                                                                                          >  2:1940479/1‑124 (MQ=255)
                                              ggCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                                                                                          <  2:1730611/140‑1 (MQ=255)
                                                cgcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                                                                                        >  2:34865/1‑140 (MQ=255)
                                                cgcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                                                                                        >  1:855689/1‑140 (MQ=255)
                                                cgcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                                                                                        <  2:1067375/140‑1 (MQ=255)
                                                cgcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                                                                                        >  2:67045/1‑140 (MQ=255)
                                                  cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGTTGCAAg                                                                                      >  1:1473497/1‑140 (MQ=255)
                                                    ggTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa                                                                                    <  2:876609/140‑1 (MQ=255)
                                                     gTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat                                                                                   >  1:1272236/1‑140 (MQ=255)
                                                     gTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat                                                                                   >  1:1522366/1‑140 (MQ=255)
                                                        gCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcat                                                                                <  2:1908041/140‑1 (MQ=255)
                                                         ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAGAGGTGCAAGTatcatc                                                                               <  1:1271228/140‑1 (MQ=255)
                                                         ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc                                                                               <  1:1058435/140‑1 (MQ=255)
                                                         ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc                                                                               <  1:2049998/140‑1 (MQ=255)
                                                          cGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTAtcatca                                                                              >  2:2040284/1‑140 (MQ=255)
                                                             gAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca                                                                           >  1:1896638/1‑140 (MQ=255)
                                                              aaaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac                                                                          <  2:1896638/140‑1 (MQ=255)
                                                                   ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGt                                                                     <  1:1045363/140‑1 (MQ=255)
                                                                   ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGt                                                                     <  2:1090365/140‑1 (MQ=255)
                                                                   ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGt                                                                     <  1:274414/140‑1 (MQ=255)
                                                                   ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGt                                                                     <  1:34865/140‑1 (MQ=255)
                                                                    gTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc                                                                    >  2:177133/1‑140 (MQ=255)
                                                                      tGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCaa                                                                  >  1:1186488/1‑140 (MQ=255)
                                                                       gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAAt                                                                 >  2:1921436/1‑140 (MQ=255)
                                                                        ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATc                                                                >  1:1249686/1‑140 (MQ=255)
                                                                        ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATc                                                                >  2:937492/1‑140 (MQ=255)
                                                                            tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAgg                                                            >  1:69096/1‑140 (MQ=255)
                                                                            tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAgg                                                            <  1:1011434/140‑1 (MQ=255)
                                                                            tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAgg                                                            <  2:1186488/140‑1 (MQ=255)
                                                                            tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAgg                                                            <  2:1272236/140‑1 (MQ=255)
                                                                                gATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATa                                                        <  1:514293/140‑1 (MQ=255)
                                                                                   atTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCa                                                     >  2:1909132/1‑140 (MQ=255)
                                                                                     tGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGc                                                   <  1:177133/140‑1 (MQ=255)
                                                                                     tGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTACAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGc                                                   >  1:1423842/1‑140 (MQ=255)
                                                                                       aaGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCa                                                 <  2:52575/139‑1 (MQ=255)
                                                                                         gACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAg                                               >  1:236542/1‑140 (MQ=255)
                                                                                         aaCCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTGCCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAg                                               <  2:570418/139‑1 (MQ=255)
                                                                                          aCCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGt                                              >  2:998585/1‑140 (MQ=255)
                                                                                           ccTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTc                                             >  1:1106643/1‑140 (MQ=255)
                                                                                           ccTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTc                                             <  2:1473497/140‑1 (MQ=255)
                                                                                            cTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTcc                                            >  1:515133/1‑140 (MQ=255)
                                                                                            cTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTcc                                            <  2:874111/140‑1 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          >  2:1183249/1‑140 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          <  1:998585/140‑1 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          <  2:1942425/140‑1 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          <  1:456774/140‑1 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          <  1:887355/140‑1 (MQ=255)
                                                                                              cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGc                                          <  1:74856/140‑1 (MQ=255)
                                                                                              aaCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCATGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTAAATCCAGGCATACCAGCCAAGTCCGc                                          <  2:551472/139‑1 (MQ=255)
                                                                                               aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCa                                         >  1:959970/1‑140 (MQ=255)
                                                                                               aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCa                                         >  1:1604219/1‑140 (MQ=255)
                                                                                                   gCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTACAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTa                                     <  2:1423842/140‑1 (MQ=255)
                                                                                                        cAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGa                                <  1:67045/140‑1 (MQ=255)
                                                                                                        cAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGa                                <  1:184216/140‑1 (MQ=255)
                                                                                                         aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGAc                               >  1:1983547/1‑140 (MQ=255)
                                                                                                                      cTTTGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGa                  >  2:136015/1‑140 (MQ=255)
                                                                                                                         tGCCATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCAGCTACGAAgg               >  2:1212493/1‑140 (MQ=255)
                                                                                                                            cATTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcgg            <  1:1921436/140‑1 (MQ=255)
                                                                                                                             aTTAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcggc           <  1:938673/140‑1 (MQ=255)
                                                                                                                              ttAACGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGgcggcg          >  1:1232374/1‑140 (MQ=255)
                                                                                                                                  cGTTTTCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTc      >  2:979300/1‑140 (MQ=255)
                                                                                                                                      ttCGAGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGaa  >  1:285247/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CGCTGGTTCACGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAA  >  NC_000913/3150011‑3150284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A