Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F48 I1 R1 66 101.5 3442112 98.9% 3404248 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,150,826 C→A A140S (GCG→TCG)  exbD ← Ton complex subunit ExbD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,150,8260CA99.0% 340.2 / ‑3.1 96A140S (GCG→TCG) exbDTon complex subunit ExbD
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (54/41);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTT  >  NC_000913/3150691‑3150960
                                                                                                                                       |                                                                                                                                      
cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTg                                                                                                                                    >  1:761222/1‑140 (MQ=255)
cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTg                                                                                                                                    >  1:1211926/1‑140 (MQ=255)
            aCCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCg                                                                                                                        >  1:615856/1‑140 (MQ=255)
            aCCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCg                                                                                                                        >  1:1480767/1‑140 (MQ=255)
                aaTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGa                                                                                                                    >  1:161335/1‑140 (MQ=255)
                 aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGAc                                                                                                                   <  1:889920/140‑1 (MQ=255)
                 aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGAc                                                                                                                   <  1:172898/140‑1 (MQ=255)
                 aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGAc                                                                                                                   >  2:1682234/1‑140 (MQ=255)
                   gCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCa                                                                                                                 <  1:798638/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:1520154/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:1211926/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:1428972/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:1468386/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:1480767/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:615856/140‑1 (MQ=255)
                      ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:761222/140‑1 (MQ=255)
                       gATGCGGCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                                             >  2:416370/1‑140 (MQ=255)
                        aTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                                            >  2:778962/1‑140 (MQ=255)
                        aTGCGTCGTGAACGACTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGTCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                                            >  2:1437482/1‑140 (MQ=255)
                          gCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              <  2:340850/136‑1 (MQ=255)
                          gCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGAc                                                                                                              >  1:340850/1‑136 (MQ=255)
                           cgtcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATc                                                                                                         >  1:1469732/1‑140 (MQ=255)
                            gtcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCt                                                                                                        >  2:1116429/1‑140 (MQ=255)
                              cgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTc                                                                                                      >  1:72459/1‑140 (MQ=255)
                               gtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCa                                                                                                     <  1:416370/140‑1 (MQ=255)
                                     gCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAc                                                                                               <  1:1567734/140‑1 (MQ=255)
                                          aTCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGcc                                                                                          >  1:1006911/1‑140 (MQ=255)
                                               gCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATg                                                                                     <  1:1161825/140‑1 (MQ=255)
                                                   aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGc                                                                                 <  2:161335/140‑1 (MQ=255)
                                                    cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGct                                                                                >  2:533796/1‑139 (MQ=255)
                                                    cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCg                                                                                >  1:28248/1‑140 (MQ=255)
                                                    cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCg                                                                                <  1:1682234/140‑1 (MQ=255)
                                                     aaaCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGt                                                                               <  1:1116429/140‑1 (MQ=255)
                                                          cACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCa                                                                          >  2:1374496/1‑140 (MQ=255)
                                                            cGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                                        >  2:1598494/1‑140 (MQ=255)
                                                                aaTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                                    <  2:1469732/139‑1 (MQ=255)
                                                                     aaCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatc                                                               >  2:614705/1‑140 (MQ=255)
                                                                     aaCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatc                                                               <  2:72459/140‑1 (MQ=255)
                                                                     aaCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatc                                                               >  2:1532998/1‑140 (MQ=255)
                                                                       cATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCaa                                                             >  1:220603/1‑140 (MQ=255)
                                                                         tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                                            >  1:235471/1‑139 (MQ=255)
                                                                         tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                                            <  2:235471/139‑1 (MQ=255)
                                                                          gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                                          >  2:1489804/1‑140 (MQ=255)
                                                                           ttGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                                         >  2:702648/1‑140 (MQ=255)
                                                                            tGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                                        >  1:522537/1‑140 (MQ=255)
                                                                            tGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                                        >  1:469272/1‑140 (MQ=255)
                                                                                aTAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTa                                                    >  1:998451/1‑140 (MQ=255)
                                                                                 tAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTaa                                                   >  1:206816/1‑140 (MQ=255)
                                                                                    aTTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                                                >  2:1526412/1‑140 (MQ=255)
                                                                                    aTTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                                                <  1:1297618/140‑1 (MQ=255)
                                                                                    aTTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                                                <  2:1526746/140‑1 (MQ=255)
                                                                                     ttttGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                                               <  2:1006911/140‑1 (MQ=255)
                                                                                     ttttGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                                               <  1:1374496/140‑1 (MQ=255)
                                                                                             gCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTg                                       >  1:1407246/1‑140 (MQ=255)
                                                                                              ccTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGt                                      >  1:1672293/1‑140 (MQ=255)
                                                                                               cTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                                     >  1:1555264/1‑140 (MQ=255)
                                                                                                 gatgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCg                                   <  2:522537/140‑1 (MQ=255)
                                                                                                   tgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                                 <  2:206816/140‑1 (MQ=255)
                                                                                                   tgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                                 <  2:1672293/140‑1 (MQ=255)
                                                                                                   tgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                                 <  2:28248/140‑1 (MQ=255)
                                                                                                   tgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                                 <  1:974118/140‑1 (MQ=255)
                                                                                                   aaaacGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                                 <  1:200123/136‑1 (MQ=255)
                                                                                                       cGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGga                             >  2:1532912/1‑140 (MQ=255)
                                                                                                       cGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGga                             >  1:791810/1‑140 (MQ=255)
                                                                                                       cGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGga                             >  2:1567206/1‑140 (MQ=255)
                                                                                                       cGTAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTACGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGga                             >  2:761908/1‑140 (MQ=255)
                                                                                                         tAGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaag                           >  2:1300171/1‑140 (MQ=255)
                                                                                                          aGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                          <  1:533796/140‑1 (MQ=255)
                                                                                                          aGCGCATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                          >  1:832992/1‑140 (MQ=255)
                                                                                                            cgcATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag                        >  2:719939/1‑140 (MQ=255)
                                                                                                            cgcATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag                        >  1:175614/1‑140 (MQ=255)
                                                                                                             gcATCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAagaaga                       <  1:1598494/140‑1 (MQ=255)
                                                                                                               aTCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg                     >  1:1680151/1‑140 (MQ=255)
                                                                                                                tCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg                    <  2:770039/140‑1 (MQ=255)
                                                                                                                tCAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg                    <  1:1532998/140‑1 (MQ=255)
                                                                                                                 cAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt                   >  2:650323/1‑140 (MQ=255)
                                                                                                                 cAGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt                   >  1:662121/1‑140 (MQ=255)
                                                                                                                  aGGCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggtg                  <  2:791810/140‑1 (MQ=255)
                                                                                                                    gCAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATggtggt                <  2:469272/140‑1 (MQ=255)
                                                                                                                     cAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGgtggtg               <  2:1555264/140‑1 (MQ=255)
                                                                                                                     cAATTCTACTTTACTTCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGgtggtg               <  1:1567206/140‑1 (MQ=255)
                                                                                                                            actttacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGtctttct        <  2:666270/140‑1 (MQ=255)
                                                                                                                              tttacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTg      >  1:371619/1‑140 (MQ=255)
                                                                                                                              tttacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTg      >  2:40471/1‑140 (MQ=255)
                                                                                                                              tttacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTg      >  2:15631/1‑140 (MQ=255)
                                                                                                                                tacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGcc    >  2:84181/1‑140 (MQ=255)
                                                                                                                                tacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGcc    <  1:650323/140‑1 (MQ=255)
                                                                                                                                tacttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGcc    >  2:424448/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTGACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:1566186/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:163079/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:1609707/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:126002/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:12092/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  2:1190823/1‑140 (MQ=255)
                                                                                                                                  cttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCtt  >  1:263372/1‑140 (MQ=255)
                                                                                                                                   ttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCt   <  2:505097/138‑1 (MQ=255)
                                                                                                                                   ttCGATTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCt   >  1:505097/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTT  >  NC_000913/3150691‑3150960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A