Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,876	C→A	P85H (CCC→CAC)	dkgA →	methylglyoxal reductase DkgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,876	0	C	A	98.5%	429.1 / NA	130	P85H (CCC→CAC)	dkgA	methylglyoxal reductase DkgA
Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (55/73); total (55/75)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAAGAGGGA  >  NC_000913/3156742‑3157012

gggTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACcgcg                                                                                                                                     <  1:1349221/140‑1 (MQ=255)

 ggTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGa                                                                                                                                    >  1:1701522/1‑140 (MQ=255)

   ttATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCGCGAAg                                                                                                                                  <  2:1957803/140‑1 (MQ=255)

   ttATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAg                                                                                                                                  <  1:321853/140‑1 (MQ=255)

   ttATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAg                                                                                                                                  <  1:625819/140‑1 (MQ=255)

   ttATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAg                                                                                                                                  <  2:779415/140‑1 (MQ=255)

   ttATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAg                                                                                                                                  <  2:1884602/140‑1 (MQ=255)

             aTTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGa                                                                                                                        >  1:592863/1‑140 (MQ=255)

             aTTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGa                                                                                                                        >  2:788340/1‑140 (MQ=255)

                 aTACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGc                                                                                                                    >  2:1606603/1‑140 (MQ=255)

                 aTACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGc                                                                                                                    >  1:256060/1‑140 (MQ=255)

                 aTACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGc                                                                                                                    <  2:574200/140‑1 (MQ=255)

                       ccgcGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGaaa                                                                                                              >  1:2109926/1‑140 (MQ=255)

                        cgcgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGaaaa                                                                                                             >  1:1406878/1‑140 (MQ=255)

                        cgcgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGaaaa                                                                                                             >  1:954749/1‑140 (MQ=255)

                            gCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACt                                                                                                         <  2:1624432/140‑1 (MQ=255)

                             ccTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTc                                                                                                        <  2:1872234/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  2:1887936/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:444615/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:815438/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:285998/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:238134/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  2:111997/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  2:2124239/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:1143125/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:1227692/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  1:1860681/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  2:592863/140‑1 (MQ=255)

                                aCAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                     <  2:1985214/140‑1 (MQ=255)

                                  aaGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCt                                                                                                   <  1:938258/140‑1 (MQ=255)

                                    gAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGACTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTg                                                                                                 <  2:2043819/140‑1 (MQ=255)

                                     aaCGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGa                                                                                                <  1:340234/140‑1 (MQ=255)

                                       cGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGAtt                                                                                              >  1:294814/1‑140 (MQ=255)

                                          agaagGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATtata                                                                                           >  2:838729/1‑140 (MQ=255)

                                            aagGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATc                                                                                         <  2:809791/140‑1 (MQ=255)

                                             agGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCg                                                                                        <  2:256060/140‑1 (MQ=255)

                                              ggTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGa                                                                                       <  2:560021/140‑1 (MQ=255)

                                              ggTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGa                                                                                       >  1:1896112/1‑140 (MQ=255)

                                              ggTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGa                                                                                       <  2:1118246/140‑1 (MQ=255)

                                               gtgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAc                                                                                      >  1:1600474/1‑140 (MQ=255)

                                               gtgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAc                                                                                      >  1:1187755/1‑140 (MQ=255)

                                               gtgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAc                                                                                      <  2:294814/140‑1 (MQ=255)

                                                tgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAcc                                                                                     <  2:1697583/140‑1 (MQ=255)

                                                 gtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                                    <  1:1040570/140‑1 (MQ=255)

                                                 gtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                                    <  2:1303969/140‑1 (MQ=255)

                                                 gtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                                    >  2:1439806/1‑140 (MQ=255)

                                                   cGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  <  2:954749/140‑1 (MQ=255)

                                                   cGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  <  2:1211480/140‑1 (MQ=255)

                                                   cGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  <  2:1984850/140‑1 (MQ=255)

                                                   cGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  <  1:788340/140‑1 (MQ=255)

                                                        aaGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTa                                                                             <  1:1045624/140‑1 (MQ=255)

                                                         aGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTatat                                                                                          <  1:1474244/126‑1 (MQ=255)

                                                         aGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTatat                                                                                          >  2:1474244/1‑126 (MQ=255)

                                                          acccTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAGCTCCAGCTTGATTATATCGACCTCTACTTAAt                                                                           <  2:656834/139‑1 (MQ=255)

                                                                     tGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCg                                                                <  2:1520079/140‑1 (MQ=255)

                                                                     tGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCCGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  >  1:194561/1‑122 (MQ=255)

                                                                     tGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCCGAAAAAACTCCAGCTTGATTATATCGACctct                                                                                  <  2:194561/122‑1 (MQ=255)

                                                                         tCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTcc                                                            <  1:1606603/140‑1 (MQ=255)

                                                                         tCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTcc                                                            <  2:1723814/140‑1 (MQ=255)

                                                                          cAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTccc                                                           >  2:1863900/1‑140 (MQ=255)

                                                                           aGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCg                                                          <  1:203800/140‑1 (MQ=255)

                                                                             tCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCt                                                        <  2:610627/140‑1 (MQ=255)

                                                                                  agagAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGa                                                   >  2:1088800/1‑140 (MQ=255)

                                                                                     gaagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTAt                                                      >  2:412316/1‑134 (MQ=255)

                                                                                     gaagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCa                                                >  1:1050670/1‑140 (MQ=255)

                                                                                     gaagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCa                                                >  1:147406/1‑140 (MQ=255)

                                                                                      aagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCAt                                               >  1:1515162/1‑140 (MQ=255)

                                                                                       agaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCAtt                                              >  2:664182/1‑140 (MQ=255)

                                                                                       agaaCTGCCCATCACCACTAAGCTGTGGATCGACGACCACAAGCGCCAACGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATTCACTGCCCCGTTCCCACTATCGACAAtt                                              >  2:548862/1‑140 (MQ=255)

                                                                                       agaaCTCTTCATCACCACTAAGCTGTGGATCGACCAACACAAGCGCCACCGCGAAGCACTGCTTGACAGTCTGAAAAACCTCCAGCCTCCTTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCAtt                                              <  1:2023220/140‑1 (MQ=255)

                                                                                           cTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGt                                          >  1:1763886/1‑140 (MQ=255)

                                                                                              ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGa                                       >  1:479323/1‑140 (MQ=255)

                                                                                              ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGa                                       >  1:2029784/1‑140 (MQ=255)

                                                                                              ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGCCCCGTTCCCGCTATCGACCATTATGTCGa                                       >  1:553385/1‑140 (MQ=255)

                                                                                                catcaCCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAg                                     <  2:1406878/140‑1 (MQ=255)

                                                                                                 atcaCCACTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGc                                    <  2:1737394/140‑1 (MQ=255)

                                                                                                     cctcTAAGCTGTGGAATGACGATCACGAGCGCCACCGTGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCGCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATgg                                <  2:1927542/137‑1 (MQ=255)

                                                                                                        cTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGaaa                             >  2:1314996/1‑140 (MQ=255)

                                                                                                        cTAAGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGaaa                             >  2:1901520/1‑140 (MQ=255)

                                                                                                           aGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGc                          >  1:213970/1‑140 (MQ=255)

                                                                                                           aGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGc                          >  2:1451741/1‑140 (MQ=255)

                                                                                                           aGCTGTGGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGc                          >  1:95779/1‑140 (MQ=255)

                                                                                                               gtgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                      <  2:213970/140‑1 (MQ=255)

                                                                                                                tgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGAt                     >  2:1546506/1‑140 (MQ=255)

                                                                                                                tgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGAt                     >  2:671753/1‑140 (MQ=255)

                                                                                                                tgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGAt                     >  2:453041/1‑140 (MQ=255)

                                                                                                                tgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGAt                     >  1:510241/1‑140 (MQ=255)

                                                                                                                tgGAACGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGAt                     >  2:636089/1‑140 (MQ=255)

                                                                                                                   aaCGACGACCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGa                  <  2:479323/140‑1 (MQ=255)

                                                                                                                       acgacCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGa                  <  2:36412/136‑1 (MQ=255)

                                                                                                                       acgacCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGa                  >  1:36412/1‑136 (MQ=255)

                                                                                                                       acgacCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTg              >  1:1880340/1‑140 (MQ=255)

                                                                                                                       acgacCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTg              >  1:1177338/1‑140 (MQ=255)

                                                                                                                          acCACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCaa           >  2:1063926/1‑140 (MQ=255)

                                                                                                                           ccACAAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCaaa          <  2:510241/140‑1 (MQ=255)

                                                                                                                            cacaAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCaaaa         >  2:398305/1‑140 (MQ=255)

                                                                                                                              caAGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAag       <  2:1880340/140‑1 (MQ=255)

                                                                                                                               aaGCGCCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAaga      <  2:1177338/140‑1 (MQ=255)

                                                                                                                                   gcCACCGCGAAGTCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACGTAATGCACTGGCCCGTCCCCGCAATCGAGCATTATGTCGAAGCATGGAAAGGCATGATCGAAGTGCAAAAAGAGGGa  <  2:21179/140‑1 (MQ=255)

                                                                                                                                   gcCACCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAAGAGGGa  <  2:1050670/140‑1 (MQ=255)

GGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAAGAGGGA  >  NC_000913/3156742‑3157012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A