Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,232,088	G→T	D142Y (GAC→TAC)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,232,088	0	G	T	99.2%	418.9 / ‑3.0	123	D142Y (GAC→TAC)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (53/69); total (53/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 125 total aligned reads displayed.

CGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGA  >  NC_000913/3231949‑3232224

  gcAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTAc                                                                                                                                        >  1:1580540/1‑140 (MQ=255)

         aGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTTGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCg                                                                                                                                 >  1:1918130/1‑140 (MQ=255)

          gCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGc                                                                                                                                <  2:963538/140‑1 (MQ=255)

                cAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCGAGTGAGGTACAATTTCGCCCGCTg                                                                                                                          >  1:1600820/1‑140 (MQ=255)

                cAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTg                                                                                                                          >  1:644008/1‑140 (MQ=255)

                  aaTTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTgcg                                                                                                                        <  2:304189/140‑1 (MQ=255)

                  aaTTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTgcg                                                                                                                        <  2:1321418/140‑1 (MQ=255)

                   aTTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTgcgc                                                                                                                       >  2:1148369/1‑140 (MQ=255)

                              cGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGgcgc                                                                                                            >  1:1065735/1‑140 (MQ=255)

                               gggCGCTACAGTTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGcgcg                                                                                                           >  1:317312/1‑140 (MQ=255)

                               gggCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGcgcg                                                                                                           >  2:2131790/1‑140 (MQ=255)

                               gggCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGcgcg                                                                                                           >  1:1926421/1‑140 (MQ=255)

                               gggCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGcgcg                                                                                                           >  2:180177/1‑140 (MQ=255)

                               gggCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGcgcg                                                                                                           <  1:1223398/140‑1 (MQ=255)

                               gagcgcTACAGCCACAAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGTCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGtgcg                                                                                                           <  2:1600820/138‑1 (MQ=255)

                                   gcTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAg                                                                                                       >  1:1092018/1‑140 (MQ=255)

                                    cTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAgg                                                                                                      <  2:2132425/140‑1 (MQ=255)

                                    cTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAgg                                                                                                      <  2:207078/140‑1 (MQ=255)

                                    cTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAgg                                                                                                      <  1:115722/140‑1 (MQ=255)

                                    cTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAgg                                                                                                      <  1:1343870/140‑1 (MQ=255)

                                       cAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAg                                                                                                   >  1:1609473/1‑140 (MQ=255)

                                       cAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAg                                                                                                   >  2:1421719/1‑140 (MQ=255)

                                          cTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGAt                                                                                                >  2:909499/1‑140 (MQ=255)

                                          cTACCAACCGCATCTGGCCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGAt                                                                                                >  1:2080175/1‑140 (MQ=255)

                                           tACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATa                                                                                               <  2:1926421/140‑1 (MQ=255)

                                           tACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATa                                                                                               <  1:322145/140‑1 (MQ=255)

                                           tACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATa                                                                                               <  1:199232/140‑1 (MQ=255)

                                           tACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAAATGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATa                                                                                               <  2:1710255/140‑1 (MQ=255)

                                            aCCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATac                                                                                              >  2:559866/1‑140 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:752691/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:457637/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:385450/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  2:1918130/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:506500/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:302813/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:1870485/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:1263698/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:1837170/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:1687769/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:180177/140‑1 (MQ=255)

                                              cAACCGCATCTGGTCGCCCCGTCCGCAGTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  1:1097468/140‑1 (MQ=255)

                                              aaaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  2:563620/139‑1 (MQ=255)

                                              aaaCCGCATCTGGTCGACCCGTCCGCATTGCAAGCCCCCATCAAACGTTTCGTGCCCAATGAGTTGAGCAACGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGTGCGCAACTGGCGCGGGAGGCAGGATacga                                                                                            <  2:299715/139‑1 (MQ=255)

                                               aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacgac                                                                                           <  1:502002/140‑1 (MQ=255)

                                               aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacgac                                                                                           >  1:1822392/1‑140 (MQ=255)

                                                aCCGCATCTGGTCGCCACGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATacgacg                                                                                          <  1:363844/140‑1 (MQ=255)

                                                 ccGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACgg                                                                                         <  2:1065735/140‑1 (MQ=255)

                                                  cGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATa                                                                                               <  1:577610/133‑1 (MQ=255)

                                                  cGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGgt                                                                                        >  2:1573080/1‑140 (MQ=255)

                                                  cGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGgt                                                                                        >  1:351738/1‑140 (MQ=255)

                                                          gTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGt                                                                                >  1:1535527/1‑140 (MQ=255)

                                                             gCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGAt                                                                             <  1:2131790/140‑1 (MQ=255)

                                                               cccGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTACAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATgg                                                                           >  1:338675/1‑140 (MQ=255)

                                                                  gTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACAGTGTAGAGGTGATGGGtt                                                                        <  1:1954065/140‑1 (MQ=255)

                                                                    ccGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTcc                                                                      >  1:1189712/1‑140 (MQ=255)

                                                                    ccGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTcc                                                                      >  1:807396/1‑140 (MQ=255)

                                                                     cGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCg                                                                     <  2:1092018/140‑1 (MQ=255)

                                                                     cGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCg                                                                     <  2:2131878/140‑1 (MQ=255)

                                                                      gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGa                                                                    <  1:909499/140‑1 (MQ=255)

                                                                      gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGa                                                                    <  2:2111274/140‑1 (MQ=255)

                                                                      gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGa                                                                    <  2:1354838/140‑1 (MQ=255)

                                                                       cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                   <  2:729113/140‑1 (MQ=255)

                                                                       cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                   <  1:595657/140‑1 (MQ=255)

                                                                       cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                   <  2:317312/140‑1 (MQ=255)

                                                                       cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                   <  2:351738/140‑1 (MQ=255)

                                                                        aTTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAg                                                                  <  2:776079/140‑1 (MQ=255)

                                                                        aTTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAg                                                                  <  1:136678/140‑1 (MQ=255)

                                                                          tGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAggg                                                                <  2:1609473/140‑1 (MQ=255)

                                                                                 ccccATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTgt                                                         >  2:1187848/1‑139 (MQ=255)

                                                                                    cATCAACCGTTTCGTGTCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCa                                                      >  2:12880/1‑140 (MQ=255)

                                                                                    cATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCa                                                      >  1:1523391/1‑140 (MQ=255)

                                                                                      acAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAAc                                                    <  2:135971/139‑1 (MQ=255)

                                                                                         aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGCGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGat                                                 >  1:1204015/1‑139 (MQ=255)

                                                                                           cGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAGCgtcag                                               >  1:2032544/1‑136 (MQ=255)

                                                                                             tttCGTGCCCCATGAGTTAAGTCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                             >  2:1917968/1‑140 (MQ=255)

                                                                                               tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                           >  2:1487716/1‑140 (MQ=255)

                                                                                               tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                           <  1:1161256/140‑1 (MQ=255)

                                                                                                cGTGTCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGa                                          <  1:12880/140‑1 (MQ=255)

                                                                                                cGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGa                                          >  1:505170/1‑140 (MQ=255)

                                                                                                  tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                        <  2:1987347/140‑1 (MQ=255)

                                                                                                    ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                      >  2:2078889/1‑140 (MQ=255)

                                                                                                    ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                      <  2:567207/140‑1 (MQ=255)

                                                                                                         tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                 <  2:338675/140‑1 (MQ=255)

                                                                                                              tAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                            <  2:1535527/140‑1 (MQ=255)

                                                                                                              tAAGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                            <  2:662111/140‑1 (MQ=255)

                                                                                                               aaGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATc                           >  2:890018/1‑140 (MQ=255)

                                                                                                                aGCCATGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                          <  2:940030/140‑1 (MQ=255)

                                                                                                                     tGAAGAGATCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                     >  2:1710213/1‑140 (MQ=255)

                                                                                                                          agaTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAGGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                >  1:180082/1‑140 (MQ=255)

                                                                                                                          agaTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                <  2:550775/140‑1 (MQ=255)

                                                                                                                            aTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                            >  2:395881/1‑126 (MQ=255)

                                                                                                                            aTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                            <  1:395881/126‑1 (MQ=255)

                                                                                                                            aTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTACTGACGCTGCGCACCAATCAGCGTAGTGACCa              >  1:1050688/1‑140 (MQ=255)

                                                                                                                            aTCCTGCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGACCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa              >  2:402784/1‑140 (MQ=255)

                                                                                                                                 gCAACTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg         >  1:852303/1‑140 (MQ=255)

                                                                                                                                    aCTGATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg      <  1:890018/140‑1 (MQ=255)

                                                                                                                                       gATCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGGGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcggca   >  2:780511/1‑139 (MQ=255)

                                                                                                                                        aTCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                <  2:352504/126‑1 (MQ=255)

                                                                                                                                        aTCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa  >  1:1529268/1‑140 (MQ=255)

                                                                                                                                        aTCTACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa  >  2:2067393/1‑140 (MQ=255)

CGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGA  >  NC_000913/3231949‑3232224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A