Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F48 I1 R1 82 50.4 1709896 99.0% 1692797 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,238,922 C→A L115M (CTG→ATG)  alx → putative membrane‑bound redox modulator Alx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,9220CA100.0% 157.9 / NA 45L115M (CTG→ATG) alxputative membrane‑bound redox modulator Alx
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/29);  total (16/29)

GCGCCGTTGCCGATCCACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCG  >  NC_000913/3238785‑3239056
                                                                                                                                         |                                                                                                                                      
gcgcCGTTGCCGATCCACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATg                                                                                                                                      >  2:172200/1‑140 (MQ=255)
gcgcCGTTGCCGATCCACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATg                                                                                                                                      >  1:320599/1‑140 (MQ=255)
         ccGATCCACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGc                                                                                                                             <  2:617331/140‑1 (MQ=255)
              ccACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCt                                                                                                                        <  1:689113/140‑1 (MQ=255)
                  aGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCggg                                                                                                                    <  2:527999/140‑1 (MQ=255)
                   ggCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCgggg                                                                                                                   <  2:741680/140‑1 (MQ=255)
                      ccTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGa                                                                                                                <  1:684709/139‑1 (MQ=255)
                      aCTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGa                                                                                                                <  1:172200/140‑1 (MQ=255)
                       cTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGAt                                                                                                               >  2:500378/1‑140 (MQ=255)
                        tGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGAtt                                                                                                              >  2:98752/1‑140 (MQ=255)
                           ccTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGtt                                                                                                           <  1:163155/140‑1 (MQ=255)
                               tctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGc                                                                                                       >  2:628497/1‑140 (MQ=255)
                                ctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCg                                                                                                      <  1:187113/140‑1 (MQ=255)
                                ctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCg                                                                                                      <  2:449823/140‑1 (MQ=255)
                                ctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCg                                                                                                      <  2:179882/140‑1 (MQ=255)
                                ctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCg                                                                                                      <  1:161864/140‑1 (MQ=255)
                                ctcACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCg                                                                                                      <  2:820715/140‑1 (MQ=255)
                                       gTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACcatc                                                                                               <  1:288455/140‑1 (MQ=255)
                                       gTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACcatc                                                                                               <  1:372961/140‑1 (MQ=255)
                                         tATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACcatcat                                                                                             >  1:709267/1‑140 (MQ=255)
                                          aTCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg                                                                                            >  2:276882/1‑140 (MQ=255)
                                                 tGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTcc                                                                                     >  2:324203/1‑139 (MQ=255)
                                                                        aCGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAg                                                              <  1:276882/140‑1 (MQ=255)
                                                                        aCGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAg                                                              <  1:576159/140‑1 (MQ=255)
                                                                              ttGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGAc                                                        >  1:789516/1‑140 (MQ=255)
                                                                               tGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACt                                                       <  1:835354/140‑1 (MQ=255)
                                                                               tGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACt                                                       <  1:508794/140‑1 (MQ=255)
                                                                                gTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTg                                                      >  1:347325/1‑140 (MQ=255)
                                                                                 tCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTgg                                                     <  1:850819/140‑1 (MQ=255)
                                                                                 tCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTgg                                                     <  2:381172/140‑1 (MQ=255)
                                                                                  cTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGa                                                    <  2:175836/140‑1 (MQ=255)
                                                                                  cTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGa                                                    <  1:627155/140‑1 (MQ=255)
                                                                                   tGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGAt                                                   <  2:709267/140‑1 (MQ=255)
                                                                                    ggCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATc                                                  <  2:287872/140‑1 (MQ=255)
                                                                                      cTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCt                                                >  2:603851/1‑140 (MQ=255)
                                                                                      cTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCt                                                >  1:346282/1‑140 (MQ=255)
                                                                                                   gCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGt                                   >  2:630248/1‑140 (MQ=255)
                                                                                                    cTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTg                                  >  1:138171/1‑140 (MQ=255)
                                                                                                            ctGTTTCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCctg                          <  2:525458/140‑1 (MQ=255)
                                                                                                              gTTCCGGCGGCATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCctgct                        >  1:570406/1‑140 (MQ=255)
                                                                                                                   ggcggcATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTa                   <  2:661882/140‑1 (MQ=255)
                                                                                                                    gcggcATTACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTAc                  >  2:257749/1‑140 (MQ=255)
                                                                                                                           tACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTg           <  2:790030/140‑1 (MQ=255)
                                                                                                                           tACAACGCCGCGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTg           <  2:819432/140‑1 (MQ=255)
                                                                                                                                  ccgcGTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATgg    >  2:114614/1‑140 (MQ=255)
                                                                                                                                    gcgTGATGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGgcg  <  1:630248/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GCGCCGTTGCCGATCCACAGGCACTGGCCTTTCTCACAGGTTATCTGATTGAGAAATCGCTGGCGGTCGATAACGTCTTTGTCTGGCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCG  >  NC_000913/3238785‑3239056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A