Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,867	G→T	Q381H (CAG→CAT)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,867	0	G	T	99.2%	422.3 / ‑5.5	123	Q381H (CAG→CAT)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (54/68); total (54/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CGGGCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGAT  >  NC_000913/3355734‑3356001

cggGCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGAc                                                                                                                                  >  1:1528309/1‑140 (MQ=255)

cggGCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGCCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGAc                                                                                                                                  >  2:1140307/1‑140 (MQ=255)

   gCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGaa                                                                                                                               <  1:258269/140‑1 (MQ=255)

       cGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTgg                                                                                                                           <  1:366686/140‑1 (MQ=255)

       cGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTgg                                                                                                                           <  1:1607883/140‑1 (MQ=255)

         tGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTc                                                                                                                         >  1:1174338/1‑140 (MQ=255)

                   cGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGcc                                                                                                               >  2:1483288/1‑140 (MQ=255)

                   cGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGcc                                                                                                               >  2:888386/1‑140 (MQ=255)

                            tGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGc                                                                                                      <  1:1432499/140‑1 (MQ=255)

                             gccgccTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACATCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGcc                                                                                                     <  2:1539079/140‑1 (MQ=255)

                                gccTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAgg                                                                                                  >  1:590993/1‑140 (MQ=255)

                                  cTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCg                                                                                                <  1:1376566/140‑1 (MQ=255)

                                    gTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGaa                                                                                              <  1:888386/140‑1 (MQ=255)

                                    gTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGaa                                                                                              <  1:1483288/140‑1 (MQ=255)

                                     tAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAAc                                                                                             <  1:1961790/140‑1 (MQ=255)

                                        ccTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGa                                                                                          <  2:1364772/140‑1 (MQ=255)

                                        ccTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGa                                                                                          <  1:1800917/140‑1 (MQ=255)

                                        acTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGa                                                                                          <  2:1614545/139‑1 (MQ=255)

                                           cGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATgg                                                                                       <  2:1137181/140‑1 (MQ=255)

                                           cGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATgg                                                                                       <  2:1677740/140‑1 (MQ=255)

                                            gACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGt                                                                                      >  1:387932/1‑140 (MQ=255)

                                            gACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGt                                                                                      <  2:1528309/140‑1 (MQ=255)

                                                 tAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCg                                                                                 >  2:734882/1‑140 (MQ=255)

                                                 tAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCg                                                                                 >  1:1734300/1‑140 (MQ=255)

                                                  aaCGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGa                                                                                >  1:1015817/1‑140 (MQ=255)

                                                      gTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACAcc                                                                            <  1:652480/140‑1 (MQ=255)

                                                      gTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACAcc                                                                            <  1:386501/140‑1 (MQ=255)

                                                           cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAg                                                                       <  1:1757838/140‑1 (MQ=255)

                                                           cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAg                                                                       <  1:987625/140‑1 (MQ=255)

                                                           cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAg                                                                       <  1:72934/140‑1 (MQ=255)

                                                              ccGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTgg                                                                    <  2:800723/140‑1 (MQ=255)

                                                                ggCGCGCTACGTCATCACCAAAGCTAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGc                                                                  <  2:1278180/140‑1 (MQ=255)

                                                                ggCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGc                                                                  <  1:2117911/140‑1 (MQ=255)

                                                                ggCGCGCTACGTCATCACCAAAGATAAGATCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGc                                                                  <  2:2016995/140‑1 (MQ=255)

                                                                 gcgcgcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGc                                                                  <  2:1694905/139‑1 (MQ=255)

                                                                 gcgcgcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGc                                                                  >  1:1694905/1‑139 (MQ=255)

                                                                 gcgcgcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCg                                                                 >  2:2009569/1‑140 (MQ=255)

                                                                     gcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTAtt                                                             >  2:38263/1‑140 (MQ=255)

                                                                      cTACGTCATCACC‑AAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCt                                                           >  2:1991302/1‑140 (MQ=255)

                                                                          gTCAGCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGGAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTAACGACACCCGCAGTGGGCGTATTCTGCa                                                        <  2:83639/140‑1 (MQ=255)

                                                                           tcatcaCCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCAc                                                       <  2:1329081/140‑1 (MQ=255)

                                                                             atcaCCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTc                                                     >  1:418527/1‑140 (MQ=255)

                                                                             atcaCCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTc                                                     >  2:1912413/1‑140 (MQ=255)

                                                                                 ccAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTc                                                     >  2:245787/1‑136 (MQ=255)

                                                                                   aaaGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGa                                               >  2:1853664/1‑140 (MQ=255)

                                                                                   aaaGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGa                                               >  2:1137270/1‑140 (MQ=255)

                                                                                   aaaGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGa                                               >  1:745379/1‑140 (MQ=255)

                                                                                   aaaGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGa                                               >  1:1341440/1‑140 (MQ=255)

                                                                                           gCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATg                                       >  2:644664/1‑140 (MQ=255)

                                                                                            cTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGa                                      >  1:739908/1‑140 (MQ=255)

                                                                                            cTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGa                                      >  2:1725029/1‑140 (MQ=255)

                                                                                            cTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGa                                      >  1:1215967/1‑140 (MQ=255)

                                                                                            cTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGa                                      >  1:268326/1‑140 (MQ=255)

                                                                                               atcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGa                                   >  2:580862/1‑140 (MQ=255)

                                                                                               atcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGa                                   >  1:243481/1‑140 (MQ=255)

                                                                                                tcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACTCCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATg                                       <  1:757515/135‑1 (MQ=255)

                                                                                                tcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATg                                       >  2:757515/1‑135 (MQ=255)

                                                                                                tcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGAt                                  >  1:1546918/1‑140 (MQ=255)

                                                                                                 caCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATc                                 >  1:2039360/1‑140 (MQ=255)

                                                                                                 caCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATc                                 >  2:792789/1‑140 (MQ=255)

                                                                                                      gcgcCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaa                            >  1:5292/1‑140 (MQ=255)

                                                                                                      gcgcCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaa                            >  2:1070162/1‑140 (MQ=255)

                                                                                                      gcgcCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaa                            >  2:616735/1‑140 (MQ=255)

                                                                                                      gcgcCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaa                            >  1:139805/1‑140 (MQ=255)

                                                                                                       cgcCTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaaa                           >  2:1053874/1‑140 (MQ=255)

                                                                                                         ccTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgc                         >  1:1189590/1‑140 (MQ=255)

                                                                                                         ccTCTGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgc                         >  1:1513885/1‑140 (MQ=255)

                                                                                                            ctGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgc                      <  1:2082550/140‑1 (MQ=255)

                                                                                                            ctGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgc                      <  2:739908/140‑1 (MQ=255)

                                                                                                             tGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgcc                     <  1:196809/140‑1 (MQ=255)

                                                                                                             tGAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgcc                     <  2:1522584/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  2:2039360/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  2:718569/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  2:268326/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:1140307/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:1424924/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  2:139805/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:1991302/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  2:1063428/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:2125529/140‑1 (MQ=255)

                                                                                                              gAAGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:363573/140‑1 (MQ=255)

                                                                                                               aaGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGccat                   >  1:1406899/1‑140 (MQ=255)

                                                                                                                aGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGccatc                  <  2:745379/140‑1 (MQ=255)

                                                                                                                aGTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGACCAGGCGAACTGATGGTTATCGACGCCCGCAGTGGGCGTATTCTGCACACGGCAGAAACCGATGACGATCTGAAAAGCCGccatc                  <  2:594055/140‑1 (MQ=255)

                                                                                                                 gTCGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    >  2:1649967/1‑137 (MQ=255)

                                                                                                                   cGGTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGccatccat               <  1:1955662/140‑1 (MQ=255)

                                                                                                                    ggTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCata              >  2:1860546/1‑140 (MQ=255)

                                                                                                                    ggTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCata              >  1:590533/1‑140 (MQ=255)

                                                                                                                     gTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCatat             <  2:1950363/140‑1 (MQ=255)

                                                                                                                     gTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCatat             <  1:670907/140‑1 (MQ=255)

                                                                                                                     gTATCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCACAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCatat             <  2:1734300/140‑1 (MQ=255)

                                                                                                                       aTCTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATaa           <  1:616735/140‑1 (MQ=255)

                                                                                                                         cTGGGATTACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAag         <  1:580862/140‑1 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    >  2:1616961/1‑122 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                    <  1:1616961/122‑1 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat  <  1:565880/140‑1 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat  <  1:913029/140‑1 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat  >  2:1187074/1‑140 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat  >  2:1626944/1‑140 (MQ=255)

                                                                                                                                tACCATCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat  >  2:1811331/1‑140 (MQ=255)

CGGGCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGTAACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGAT  >  NC_000913/3355734‑3356001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A