Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,928	C→A	R402S (CGC→AGC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,928	0	C	A	100.0%	193.8 / NA	55	R402S (CGC→AGC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (24/31); total (24/31)

TGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGG  >  NC_000913/3355791‑3356058

tGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGc                                                                                                                                  >  1:795510/1‑140 (MQ=255)

  cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCCCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACcagcag                                                                                                                                <  1:708027/140‑1 (MQ=255)

  cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACcagcag                                                                                                                                >  1:523109/1‑140 (MQ=255)

  cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACcagcag                                                                                                                                <  1:709843/140‑1 (MQ=255)

  cGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCat                                                                                                                                >  2:973132/1‑139 (MQ=255)

     ccGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTgg                                                                                                                             <  1:948741/140‑1 (MQ=255)

       ggCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGc                                                                                                                           <  2:1071050/140‑1 (MQ=255)

       ggCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGc                                                                                                                           <  1:515864/140‑1 (MQ=255)

       ggCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCGCTGAAGGCGGGATCTGGGATTACCAGCCTGCCGAAGTAGTCGAAAAAGGCAGCGTCGGGCCAGGCGAACAGATGGTTATCGACAACAGCAGTTGGc                                                                                                                           <  2:651510/140‑1 (MQ=255)

        gcgcgcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCg                                                                                                                          >  1:709169/1‑140 (MQ=255)

        gcgcgcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCg                                                                                                                          >  1:535093/1‑140 (MQ=255)

            gcTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTAtt                                                                                                                      <  2:823422/140‑1 (MQ=255)

                        ccAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCa                                                                                                          >  1:413489/1‑140 (MQ=255)

                               tAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCg                                                                                                   <  2:867683/140‑1 (MQ=255)

                                       tcaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGAt                                                                                           >  2:1274192/1‑140 (MQ=255)

                                       acaCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGAt                                                                                           <  2:413489/139‑1 (MQ=255)

                                        caCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATc                                                                                          <  1:9325/140‑1 (MQ=255)

                                        caCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATc                                                                                          >  2:282332/1‑140 (MQ=255)

                                         aCCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGTCGATCt                                                                                         <  2:68635/140‑1 (MQ=255)

                                             gcgcCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGaaa                                                                                     >  1:798544/1‑140 (MQ=255)

                                                ccTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgc                                                                                  >  1:1036546/1‑140 (MQ=255)

                                                   ctGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTAAACTGCACTCGGCAGAAACCGATGACGATCTTAAAAgccgc                                                                               <  2:643809/140‑1 (MQ=255)

                                                    tGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgcc                                                                              <  2:1254110/140‑1 (MQ=255)

                                                    tGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAgccgcc                                                                              <  2:674672/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCGGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:225785/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  2:1161387/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:1203304/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:282332/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:569441/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:365196/140‑1 (MQ=255)

                                                     gAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGcca                                                                             <  1:41327/140‑1 (MQ=255)

                                                          cGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGccatccat                                                                        <  2:447823/140‑1 (MQ=255)

                                                            gTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCatat                                                                      <  2:709169/140‑1 (MQ=255)

                                                             tATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCAtata                                                                     >  2:816547/1‑140 (MQ=255)

                                                                 tGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAaga                                                                 <  2:1262304/140‑1 (MQ=255)

                                                                   ggATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGt                                                               >  1:94661/1‑140 (MQ=255)

                                                                       tACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat                                                           >  2:809266/1‑140 (MQ=255)

                                                                             ccTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGaa                                                     >  1:871164/1‑140 (MQ=255)

                                                                               tGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGaaaa                                                   >  1:96317/1‑140 (MQ=255)

                                                                                      gTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTccg                                            <  2:94661/140‑1 (MQ=255)

                                                                                      gTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTccg                                            <  2:1036546/140‑1 (MQ=255)

                                                                                        ggTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTccgcc                                          <  1:389477/140‑1 (MQ=255)

                                                                                         gTCGAAAAAGGCCGCGTCGGGCTAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGGGGATGGGGAAAAACGCccgcgg                                         >  1:1226173/1‑138 (MQ=255)

                                                                                         gTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTccgccg                                         >  1:80772/1‑140 (MQ=255)

                                                                                         gTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTccgccg                                         >  1:93163/1‑140 (MQ=255)

                                                                                                aaGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTa                                  <  2:523109/140‑1 (MQ=255)

                                                                                                aaGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTa                                  <  1:665444/140‑1 (MQ=255)

                                                                                                 aGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTAc                                 >  1:365246/1‑140 (MQ=255)

                                                                                                 aGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTAc                                 >  1:839231/1‑140 (MQ=255)

                                                                                                  ggCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTAcc                                <  2:80772/140‑1 (MQ=255)

                                                                                                   gCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCg                               >  1:1278080/1‑140 (MQ=255)

                                                                                                       cgTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTg                           <  2:96317/140‑1 (MQ=255)

                                                                                                        gTCGGGCCAGGCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGa                          >  2:544904/1‑140 (MQ=255)

                                                                                                                 ggCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGcc                 <  2:275251/140‑1 (MQ=255)

                                                                                                                  gCGAACTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGccc                >  1:554416/1‑140 (MQ=255)

                                                                                                                      aCTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATg            <  1:816547/140‑1 (MQ=255)

                                                                                                                       cTGATGGTTATCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATga           >  1:333517/1‑140 (MQ=255)

                                                                                                                                aTCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTggg  <  2:365246/140‑1 (MQ=255)

                                                                                                                                aTCGACACCAGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTggg  >  2:712309/1‑140 (MQ=255)

TGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATCACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGCCGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGG  >  NC_000913/3355791‑3356058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A