Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,513	G→T	S114* (TCG→TAG)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,513	0	G	T	98.0%	160.6 / ‑2.8	50	S114* (TCG→TAG)	chiA	endochitinase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (47/2); total (48/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

GGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCA  >  NC_000913/3469386‑3469646

ggCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAg                                                                                                                           >  1:880853/1‑140 (MQ=255)

ggCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAg                                                                                                                           >  1:1856358/1‑140 (MQ=255)

  ctccccACCAGGCATTTTTAAATACCGCACCATAAAAGACGAAACCCCAGGTCTAACCGCCCTGCTGTTTATTCCGGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGATGGTGTGGCCTAACCGCCATTAGcc                                                                                                                         <  2:500962/137‑1 (MQ=255)

  cTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGcc                                                                                                                         >  1:960802/1‑140 (MQ=255)

  cTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGcc                                                                                                                         >  1:934647/1‑140 (MQ=255)

   accccTCCAGGATTTTTTATATCCCGCACCATAAAAGACAACATACCGGGTCTGCCCGCCCTGTTTTTTTTTCCAGACACCTACAAAAAAATAAGCCGGAACAGTGCCCTGTGCTGGTGTAGCCAAACCGCCATTAGCCg                                                                                                                        <  2:1321460/138‑1 (MQ=255)

          caGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTTCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTAtt                                                                                                                 >  2:907814/1‑140 (MQ=255)

          agggttttttttTATACCCCCCAATAAAAGACAAAAAACCGGGTCGGCCCCCCCTGCTTTTTTTTCCGGGAAACTAAAGAAGAATTAGGCGGAAAAGTGCCCTGTGGTGGTGTCCCTAAACCGCCATTAGCCGGCGTAtt                                                                                                                 <  2:1151826/134‑1 (MQ=255)

          aaGGCATTTTTATATCCCCCACCATTAAAGACAACACACCGGGTCTGCCCGCCCTGCTTTTTTTTCCGGGCAACTACAAAAAAATTGGACGGAACAGTGCCTGGTGCTGGTGTACCCTACCCGCCATTAGCCGGCGTAtt                                                                                                                 <  2:1151827/139‑1 (MQ=255)

           aGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTg                                                                                                                >  2:150422/1‑140 (MQ=255)

               aTTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATg                                                                                                            >  1:1373915/1‑140 (MQ=255)

                  tttATATCCCGAACAATAAAAGACAAAAAACAAGGTCTGCCCGCCTTGCTTTTTATCCAAGGCAACAAAAAAAGATTAAGCCGGAACAGTGCCCGGTGTTGGTGAAGCAAAACCGCCATTAGCCGGCGTATTGGATGCAg                                                                                                         <  2:934647/140‑1 (MQ=255)

                       ataCCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTa                                                                                                    >  1:330260/1‑140 (MQ=255)

                         aCCGCACAATAAAAGACAACATCCCGGGTCTGCCCGCCCTGTTTTTTATTCCAGGCAACAACAAAAAAATTAGACGGAACAGTGCCTTGTGTTGGTGTACCTTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatt                                                                                                  <  2:56220/140‑1 (MQ=255)

                           cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttt                                                                                                >  2:598746/1‑139 (MQ=255)

                           cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttg                                                                                                >  1:151995/1‑140 (MQ=255)

                           cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttg                                                                                                >  2:1380319/1‑140 (MQ=255)

                           cGCACCATTAAAGACGACATACCAGGCCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttg                                                                                                >  2:921326/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgat                                                                                              >  2:1796846/1‑139 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  1:56249/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  2:1130703/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  2:1133210/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  2:1592193/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  2:306889/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  1:1955239/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  1:1756251/1‑140 (MQ=255)

                             cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGAGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaa                                                                                              >  2:2103216/1‑140 (MQ=255)

                                 ataaaaGACGACATACCGGGTCTGCCCGCCTTGTTTTTTATTCCGGGCAACTAAAAAAAAATAAGACGAAACAGTCCCGTGTGTGGGTGTACCAAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaatttg                                                                                          <  2:1525971/137‑1 (MQ=255)

                                  ttAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaatttga                                                                                         >  1:1254626/1‑140 (MQ=255)

                                          gacATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATg                                                                                 >  2:1187162/1‑140 (MQ=255)

                                          gacATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATg                                                                                 >  1:31015/1‑140 (MQ=255)

                                          aaaaTACCGGGTCTCCCCGCCTTGTTTTTTATCCCAGAAAACAAAAAAAAAATAAGACGGAACGGTGCCTTGTGTTGGTGTACCTTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATg                                                                                 <  2:515213/137‑1 (MQ=255)

                                            cATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAg                                                                               >  2:1474104/1‑140 (MQ=255)

                                            cATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAg                                                                               >  1:515213/1‑140 (MQ=255)

                                            cATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAg                                                                               >  1:662519/1‑140 (MQ=255)

                                            cATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAg                                                                               >  1:1645502/1‑140 (MQ=255)

                                                     tCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGctc                                                                      >  2:2090054/1‑140 (MQ=255)

                                                      cTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGctct                                                                     >  2:719263/1‑140 (MQ=255)

                                                         aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCtttt                                                                  >  1:787391/1‑140 (MQ=255)

                                                         aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCtttt                                                                  >  1:823344/1‑140 (MQ=255)

                                                         aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCtttt                                                                  >  1:926683/1‑140 (MQ=255)

                                                         aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCtttt                                                                  >  2:1272062/1‑140 (MQ=255)

                                                         aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCtttt                                                                  >  1:248268/1‑140 (MQ=255)

                                                                 gttttttATTCAAGGAAACAAAAAAAAAATAAGCGGAAACGGTCCCCGGTCGTGGTGTACCTAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                                          <  2:31015/136‑1 (MQ=255)

                                                                  ctgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaaa                                                         >  1:579997/1‑140 (MQ=255)

                                                                   tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCCCTTTTCGCAGGaaat                                                        >  2:33292/1‑139 (MQ=255)

                                                                          tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTg                                                 >  1:78550/1‑140 (MQ=255)

                                                                              ggCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGCGTAGCCTAACCGCCATTATCCGGCGTATTGGGTGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaa                                             >  1:1520362/1‑140 (MQ=255)

                                                                               gCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                                            >  1:718012/1‑140 (MQ=255)

                                                                               gCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                                            >  1:1694742/1‑140 (MQ=255)

                                                                               gCAACAAAAAAAGAATTAGCCGGAACGGTGCTCTGGTGTGGTGTAGCTTACCCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                                            <  2:1856358/140‑1 (MQ=255)

                                                                                      cAGAAGAATTAGACGAAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTa                                     <  1:150422/140‑1 (MQ=255)

                                                                                              ttAGACGGAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                             <  1:719263/140‑1 (MQ=255)

                                                                                              ttAGACGGAACAGTCCCTTGTGCTGGTGTCGCAAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                             <  1:1237569/140‑1 (MQ=255)

                                                                                              ttAGACGAAACAGTGCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                             <  1:1133210/140‑1 (MQ=255)

                                                                                                aGACGAAACAGTGCCGTGTGTTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagcag                           <  2:1254626/140‑1 (MQ=255)

                                                                                                            gCCCTGTGCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAc               <  1:1474104/140‑1 (MQ=255)

                                                                                                                tgtgCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCtt           >  1:1918336/1‑140 (MQ=255)

                                                                                                                  tgCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTa         >  1:1043663/1‑140 (MQ=255)

                                                                                                                  tgCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTa         >  1:88149/1‑140 (MQ=255)

                                                                                                                  tgCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTa         >  2:2003303/1‑140 (MQ=255)

                                                                                                                  tgCTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTa         >  1:1313542/1‑140 (MQ=255)

                                                                                                                    cTGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTaaa       <  2:662519/140‑1 (MQ=255)

                                                                                                                     tGGTGTAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAAc      >  2:1649909/1‑140 (MQ=255)

                                                                                                                       gtgtAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGc    >  1:1301880/1‑140 (MQ=255)

                                                                                                                         gtAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCa  <  2:1301880/140‑1 (MQ=255)

                                                                                                                         gtAGCCTAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCa  <  1:921326/140‑1 (MQ=255)

GGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCA  >  NC_000913/3469386‑3469646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A