Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,340	G→T	L177M (CTG→ATG)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,340	0	G	T	98.8%	260.4 / ‑5.8	85	L177M (CTG→ATG)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (45/39); minor base C (0/1); total (45/40)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

ATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGA  >  NC_000913/3535203‑3535474

atGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATcc                                                                                                                                      >  1:2065262/1‑140 (MQ=255)

      aaaGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCTGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGAGCTACCAACTGTCTTTTCTGGATACGAATAAACATCCACGccc                                                                                                                                >  2:2023820/1‑140 (MQ=255)

      aaaGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGccc                                                                                                                                <  2:767183/140‑1 (MQ=255)

           cACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAACCATCCACGCCCCGCCt                                                                                                                           <  2:1462676/140‑1 (MQ=255)

           cACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCt                                                                                                                           >  1:746111/1‑140 (MQ=255)

           cACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCt                                                                                                                           >  2:246339/1‑140 (MQ=255)

           cACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCACCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGGTCGAGTTCGACCAACGGTCGGTTCTGGATACGAATAAACTTCCACGCCCCGCCt                                                                                                                           >  2:381178/1‑140 (MQ=255)

           aaCGGGTAACGGAACGCACCTCCGAAGCGCAATACTCACGCAGCGGCGGCGAAATAACCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCt                                                                                                                           <  2:588217/139‑1 (MQ=255)

             gggttAACGGAACGCACCCCCGAAGCGCCATACTCTCGCGGCGGCGGCGGAATAATCCCTTCACAAACTTGAAAGGCTGCGTGTTCGAGCTCGACCAACGGTCGGTATTGGAAACAAAAAAACACCCACGCCCCGCCTAt                                                                                                                         <  2:924630/136‑1 (MQ=255)

             cGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTAt                                                                                                                         >  2:1564842/1‑140 (MQ=255)

             cGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTAt                                                                                                                         >  2:1885485/1‑140 (MQ=255)

              gggTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATc                                                                                                                        <  1:378300/140‑1 (MQ=255)

              gggTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATc                                                                                                                        >  1:845924/1‑140 (MQ=255)

              gggTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATc                                                                                                                        >  1:1259473/1‑140 (MQ=255)

              gggTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATc                                                                                                                        >  1:1215870/1‑140 (MQ=255)

                 aaaCGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGcc                                                                                                                     <  2:845924/139‑1 (MQ=255)

                     ggAACGCACCCCGGAAGCGCCATACTCACGAAGCGGGGGGGGAATAATCCCTTTACAACCCTGCACGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATa                                                                                                                 <  1:2023820/140‑1 (MQ=255)

                          gCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATa                                                                                                            >  2:656059/1‑140 (MQ=255)

                          gCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATa                                                                                                            >  1:501770/1‑140 (MQ=255)

                          gCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGATTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATa                                                                                                            >  2:830479/1‑140 (MQ=255)

                          ccACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGAAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATa                                                                                                            <  2:1835132/139‑1 (MQ=255)

                               tCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGc                                                                                                       <  2:746111/140‑1 (MQ=255)

                               tCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGc                                                                                                       >  1:742751/1‑140 (MQ=255)

                               tCCGAAGCGCAATCCCCACCAAGCGGCGGCGGAATAAACCCTTTCCCAACCTGCAAGCCTGCGTGTTCGAGATCGCCAAACGGTCGGTTCTGAATACGAATAACCATCCACGCCCCGCCTATCGCCAATAGCATAATCGc                                                                                                       <  2:293310/140‑1 (MQ=255)

                                 cGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCa                                                                                                     <  2:611785/140‑1 (MQ=255)

                                 cGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCa                                                                                                     <  1:1885485/140‑1 (MQ=255)

                                      cgcCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCg                                                                                                  <  1:301371/138‑1 (MQ=255)

                                      cgcCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCg                                                                                                  >  2:301371/1‑138 (MQ=255)

                                        ccATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGtata                                                                                              >  1:1146357/1‑140 (MQ=255)

                                        ccATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGtata                                                                                              >  1:2004685/1‑140 (MQ=255)

                                         cATACTCACGCAGCGGCGGCGGAATAATCCCTTTCCCACCCTGAAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAg                                                                                             <  2:703961/140‑1 (MQ=255)

                                         cATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAg                                                                                             >  2:1500779/1‑140 (MQ=255)

                                              tCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGaa                                                                                        <  2:1322653/140‑1 (MQ=255)

                                              tCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGaa                                                                                        <  1:656059/140‑1 (MQ=255)

                                                aCGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACa                                                                                      >  1:1104843/1‑140 (MQ=255)

                                                aCGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACa                                                                                      >  1:347242/1‑140 (MQ=255)

                                                aCGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACa                                                                                      >  1:622297/1‑140 (MQ=255)

                                                  gCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGc                                                                                    >  2:1941291/1‑140 (MQ=255)

                                                      cggcggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGgag                                                                                >  2:1783859/1‑140 (MQ=255)

                                                      cggcggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGgag                                                                                >  2:654541/1‑140 (MQ=255)

                                                      cggcggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGgag                                                                                <  1:412224/140‑1 (MQ=255)

                                                        gcggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGgagag                                                                              >  1:1177907/1‑140 (MQ=255)

                                                            cggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAt                                                                          >  1:31522/1‑140 (MQ=255)

                                                                 taatCCCTTTACAAACCGGCAAGGCTGCTTTTTGGAGAGCGACCAGCGGTCGGTTCTGGATCCGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGTGGAACAGCGGAGAGAAATCGccc                                                                     <  1:495147/140‑1 (MQ=255)

                                                                           aCCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAAttt                                                           >  1:1236318/1‑140 (MQ=255)

                                                                               aCCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGt                                                       >  2:465505/1‑140 (MQ=255)

                                                                                ccTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTc                                                      >  2:781679/1‑140 (MQ=255)

                                                                                    cAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCg                                                  >  1:1913190/1‑140 (MQ=255)

                                                                                    cAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCg                                                  >  2:1099338/1‑140 (MQ=255)

                                                                                     aagccggCGTGTTCGAGACCGACCAACGGTCGGTTCTGGATACAAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCgg                                                 <  2:501770/134‑1 (MQ=255)

                                                                                     aaGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCgg                                                 <  2:31522/140‑1 (MQ=255)

                                                                                     aaGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCgg                                                 <  1:781679/140‑1 (MQ=255)

                                                                                     aaGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCgg                                                 <  2:742751/140‑1 (MQ=255)

                                                                                     aaGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCgg                                                 <  2:1215870/140‑1 (MQ=255)

                                                                                         cTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcg                                             <  2:1072532/140‑1 (MQ=255)

                                                                                         cTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcg                                             >  2:1008386/1‑140 (MQ=255)

                                                                                          tGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgc                                            <  2:1969518/140‑1 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           <  2:1177907/140‑1 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           <  2:1146357/140‑1 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           >  2:1079204/1‑140 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           >  1:1058955/1‑140 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           <  1:1008386/140‑1 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACACCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           <  2:808397/140‑1 (MQ=255)

                                                                                           gCGTGTTCGAGATCGACCAACGGTCGATTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCAcgcg                                           <  1:830479/140‑1 (MQ=255)

                                                                                             gtgtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTa                                         <  2:622297/140‑1 (MQ=255)

                                                                                             gtgtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTa                                         <  1:654541/140‑1 (MQ=255)

                                                                                               tttCGAGATAGCCCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAcc                                       <  2:2004685/139‑1 (MQ=255)

                                                                                               gtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCTATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAcc                                       <  1:688585/140‑1 (MQ=255)

                                                                                               gtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAcc                                       <  1:136701/140‑1 (MQ=255)

                                                                                               gtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAcc                                       <  1:465505/140‑1 (MQ=255)

                                                                                                ttCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAccc                                      <  2:1913190/140‑1 (MQ=255)

                                                                                                   gagaTCGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGa                                   >  2:1000024/1‑140 (MQ=255)

                                                                                                        cGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTg                              >  1:1049354/1‑140 (MQ=255)

                                                                                                        cGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTg                              >  1:1960146/1‑140 (MQ=255)

                                                                                                        cGACCAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTg                              >  2:413969/1‑140 (MQ=255)

                                                                                                           ccAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGc                           <  1:1564842/140‑1 (MQ=255)

                                                                                                           ccAACGGTCGGTTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGc                           >  1:1410144/1‑140 (MQ=255)

                                                                                                                ggtcggtTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAg                      >  2:1814255/1‑140 (MQ=255)

                                                                                                                  tcggtTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGcc                    <  1:1140854/140‑1 (MQ=255)

                                                                                                                     gtTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAgt                 >  2:676576/1‑139 (MQ=255)

                                                                                                                     gtTCTGGATACGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAgg                 >  2:1039487/1‑140 (MQ=255)

                                                                                                                       ccTGTATACGAATAATCATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGtt               <  1:381178/139‑1 (MQ=255)

                                                                                                                             aaCGAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGc         <  2:2065262/139‑1 (MQ=255)

                                                                                                                                gAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAg      >  1:133313/1‑140 (MQ=255)

                                                                                                                                gAATAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAg      >  2:833448/1‑140 (MQ=255)

                                                                                                                                  aTAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac    >  1:788339/1‑140 (MQ=255)

                                                                                                                                  aTAAACATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac    >  2:20376/1‑140 (MQ=255)

                                                                                                                                   aaaaCATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgacg   <  1:676576/139‑1 (MQ=255)

                                                                                                                                    aaaCATCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgacga  <  1:1079204/140‑1 (MQ=255)

ATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGA  >  NC_000913/3535203‑3535474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A