Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F48 I1 R1	56	123.4	4244010	99.1%	4205813	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,853	G→T	A157S (GCC→TCC)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,853	0	G	T	99.2%	459.9 / ‑6.5	132	A157S (GCC→TCC)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (68/63); minor base A (0/1); total (68/64)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 134 total aligned reads displayed.

CGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGA  >  NC_000913/3537718‑3537986

cgcACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCcgc                                                                                                                                   >  1:977950/1‑140 (MQ=255)

cgcACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCcgc                                                                                                                                   >  1:2087976/1‑140 (MQ=255)

cgcACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCcgc                                                                                                                                   >  1:1522681/1‑140 (MQ=255)

  cACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCgcgc                                                                                                                                 >  2:463448/1‑140 (MQ=255)

        cgGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATg                                                                                                                           >  2:1784098/1‑140 (MQ=255)

          ggCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgc                                                                                                                         >  1:185554/1‑140 (MQ=255)

           gCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcg                                                                                                                        <  1:2061838/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  2:2033108/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  2:1007977/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  1:1223160/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  1:2015115/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  1:1784098/140‑1 (MQ=255)

            cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAACCGCGCTGGATGgcgc                                                                                                                       <  2:1406158/140‑1 (MQ=255)

            aaaaTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  2:913821/139‑1 (MQ=255)

            aaaaTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgc                                                                                                                       <  2:276125/139‑1 (MQ=255)

             aaaTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGgcgcg                                                                                                                      <  2:272111/140‑1 (MQ=255)

                            cAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATg                                                                                                       >  1:1677753/1‑140 (MQ=255)

                              gggCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGa                                                                                                     >  2:1616883/1‑140 (MQ=255)

                              gggCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGa                                                                                                     >  1:210751/1‑140 (MQ=255)

                               ggCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGaa                                                                                                    >  2:1894821/1‑140 (MQ=255)

                                   tGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGt                                                                                                >  2:1779921/1‑140 (MQ=255)

                                    gAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGtt                                                                                               >  2:692941/1‑140 (MQ=255)

                                         gTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCg                                                                                          >  2:1634755/1‑140 (MQ=255)

                                          ttGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGa                                                                                         >  1:1296741/1‑140 (MQ=255)

                                               tGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATg                                                                                    >  1:521777/1‑140 (MQ=255)

                                                    tgctgTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg                                                                               <  2:1062664/140‑1 (MQ=255)

                                                    tgctgTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg                                                                               <  1:114777/140‑1 (MQ=255)

                                                      ctgTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCgct                                                                             <  2:1428638/140‑1 (MQ=255)

                                                      ctgTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCgct                                                                             <  2:1296741/140‑1 (MQ=255)

                                                        gtgGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCgctgc                                                                           <  1:851638/140‑1 (MQ=255)

                                                           gAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTgg                                                                        >  1:798134/1‑140 (MQ=255)

                                                              cGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGa                                                                     <  1:1713976/140‑1 (MQ=255)

                                                              cGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGa                                                                     <  1:2045593/140‑1 (MQ=255)

                                                              agATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGa                                                                     <  2:1102926/139‑1 (MQ=255)

                                                                aTCCGTCACACACGCCGGAAGGCGCCGCTGCACAATATGTTTATGCCGATAAAAGCGTGGCAGATACCAAAACCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTTCCGAATATGCCGCCCTCCTGGCGaaa                                                                   >  2:2107433/1‑140 (MQ=255)

                                                                aTCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGTTAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGaaa                                                                   >  1:1580917/1‑140 (MQ=255)

                                                                aTCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGaaa                                                                   >  1:470201/1‑140 (MQ=255)

                                                                aTCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGaaa                                                                   >  2:1302465/1‑140 (MQ=255)

                                                                aTCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGaaa                                                                   >  1:1215533/1‑140 (MQ=255)

                                                                aTCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGaaa                                                                   >  2:487944/1‑140 (MQ=255)

                                                                  ccGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGt                                                                 <  2:890950/140‑1 (MQ=255)

                                                                    gTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGc                                                               <  1:1071925/140‑1 (MQ=255)

                                                                        cacacGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTgt                                                           >  2:612475/1‑139 (MQ=255)

                                                                        cacacGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGa                                                           <  2:470201/140‑1 (MQ=255)

                                                                          aacGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGAtt                                                         <  2:1006379/139‑1 (MQ=255)

                                                                            cGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTAt                                                       <  2:1051157/140‑1 (MQ=255)

                                                                            cGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTAt                                                       <  1:717374/140‑1 (MQ=255)

                                                                            cGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTAt                                                       >  1:407869/1‑140 (MQ=255)

                                                                             gCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATc                                                      >  2:1841481/1‑140 (MQ=255)

                                                                               cAGAATTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCtg                                                    >  2:1915421/1‑140 (MQ=255)

                                                                               cAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCtg                                                    >  2:2113360/1‑140 (MQ=255)

                                                                                       ggcgcTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATTGCGCGCGCTATATCCTGATGGAACGGTTTACCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGgaagaa                                            >  2:1419149/3‑140 (MQ=255)

                                                                                       gccgcTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGgaagaa                                            >  1:1861890/1‑140 (MQ=255)

                                                                                       gccgcTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGgaagaa                                            >  1:1320979/1‑140 (MQ=255)

                                                                                       gccgcTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGgaagaa                                            >  1:537431/1‑140 (MQ=255)

                                                                                         cgcTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAAcg                                          >  1:234322/1‑140 (MQ=255)

                                                                                           cTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAAcgcg                                        <  1:698459/140‑1 (MQ=255)

                                                                                              cacaATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCAt                                     <  1:2077391/140‑1 (MQ=255)

                                                                                              cacaATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCAt                                     >  2:1231399/1‑140 (MQ=255)

                                                                                               acaATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCAtt                                    <  1:1023082/140‑1 (MQ=255)

                                                                                                caATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCAttt                                   <  1:558577/140‑1 (MQ=255)

                                                                                                aaaTATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCAttt                                   <  2:894014/139‑1 (MQ=255)

                                                                                                 aaTATGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCAGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTg                                  <  2:1677753/140‑1 (MQ=255)

                                                                                                   tatGTTTATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGt                                <  2:2087976/140‑1 (MQ=255)

                                                                                                       tttATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCt                            >  2:1291484/1‑140 (MQ=255)

                                                                                                        ttATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCtt                           >  2:698242/1‑139 (MQ=255)

                                                                                                        ttATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTa                           >  2:1071070/1‑140 (MQ=255)

                                                                                                        ttATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTa                           <  1:415248/140‑1 (MQ=255)

                                                                                                        ttATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTa                           <  2:1522681/140‑1 (MQ=255)

                                                                                                         tATGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTAc                          >  1:1641589/1‑140 (MQ=255)

                                                                                                           tGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGt                                <  1:9110/132‑1 (MQ=255)

                                                                                                           tGCCGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACgg                        >  1:849289/1‑140 (MQ=255)

                                                                                                             ccGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTTGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtg                      >  1:530695/1‑140 (MQ=255)

                                                                                                             ccGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtt                      >  2:857914/1‑139 (MQ=255)

                                                                                                             ccGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtg                      >  1:831464/1‑140 (MQ=255)

                                                                                                             ccGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtg                      <  2:185279/140‑1 (MQ=255)

                                                                                                             ccGATAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtg                      >  1:1521300/1‑140 (MQ=255)

                                                                                                                aTAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGgtggtg                   <  1:1841481/140‑1 (MQ=255)

                                                                                                                 tAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGGAGAACGCGCATTTGGTTTCTACGGTGGTGa                  >  1:487866/1‑140 (MQ=255)

                                                                                                                 tAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtggt                    <  2:974652/138‑1 (MQ=255)

                                                                                                                 tAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACggtggt                    >  1:974652/1‑138 (MQ=255)

                                                                                                                 tAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGa                  >  1:1292999/1‑140 (MQ=255)

                                                                                                                 tAAAGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGa                  >  2:715853/1‑140 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAg                 <  2:1861890/140‑1 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAg                 <  2:1823634/140‑1 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAg                 <  2:407869/140‑1 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAg                 <  2:1641589/140‑1 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAg                 <  2:537431/140‑1 (MQ=255)

                                                                                                                  aaaGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGAATTTGGTTTCTACGGTGGTGAg                 <  2:615599/140‑1 (MQ=255)

                                                                                                                    aGGCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCg               <  1:625280/140‑1 (MQ=255)

                                                                                                                     ggCGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCgg              <  1:1753953/140‑1 (MQ=255)

                                                                                                                       cGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTa            <  1:1071070/140‑1 (MQ=255)

                                                                                                                       cGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTa            <  1:487944/140‑1 (MQ=255)

                                                                                                                       cGTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTa            <  2:2018101/140‑1 (MQ=255)

                                                                                                                        gTGGCAGATACCAAATCCGCGCTGGATGGCGCGCGCCATATCCTGATGGAACGGTTTCCCGAAGATGCCGCGCTGCTGGCGAAAGCGCGTGATTATCTGTGGAAGAACGCGCCTTTGGTTTCTACGGTTGTGGGCGGTaa           >  2:1509508/1‑140 (MQ=255)

                                                                                                                          ggCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTaa           >  1:1780885/1‑138 (MQ=255)

                                                                                                                          ggCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTaa           <  2:1780885/138‑1 (MQ=255)

                                                                                                                          ggCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAaag         >  2:2044026/1‑140 (MQ=255)

                                                                                                                           gCAGATACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAaaga        >  1:645477/1‑140 (MQ=255)

                                                                                                                                tACCAAATCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAgg   >  2:1191810/1‑140 (MQ=255)

CGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGAAAGTGCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGA  >  NC_000913/3537718‑3537986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A