Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,645,155	C→A	E10* (GAA→TAA)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,645,155	0	C	A	99.2%	424.6 / NA	124	E10* (GAA→TAA)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (49/74); minor base T (1/0); total (50/74)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACG  >  NC_000913/3645019‑3645282

ggCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTaaaa                                                                                                                              <  1:1068150/140‑1 (MQ=255)

    ggCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGa                                                                                                                          <  1:1621595/140‑1 (MQ=255)

      cAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGt                                                                                                                        <  2:2003550/140‑1 (MQ=255)

           aTTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTa                                                                                                                   <  1:1198904/140‑1 (MQ=255)

                        tACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCAt                                                                                                      <  2:571677/140‑1 (MQ=255)

                          cGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTc                                                                                                    >  1:1700435/1‑140 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:1700435/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:1226516/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:1043595/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:104024/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:579955/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:554287/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:2120660/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:1986478/140‑1 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 >  2:2136692/1‑140 (MQ=255)

                             tGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:552601/140‑1 (MQ=255)

                             ggCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  2:614476/139‑1 (MQ=255)

                             ggCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:666745/139‑1 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCGACG‑CTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCGGGCACGACATGATCCGGGAGAATTTTAGCAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATATGCGCa                                                                                               >  2:106859/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGGACGACATGTTCCGGGAGAATTTTTGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGAATCGTCATTCGCGCa                                                                                               >  1:2150948/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGGATTTTAGAAAACGGAGGCAATTTAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:1282856/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:1588343/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:1191216/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:651328/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:858852/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:1096843/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  2:1867730/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  2:1328751/1‑140 (MQ=255)

                               ccccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCa                                                                                               >  1:1763787/1‑140 (MQ=255)

                                cccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAg                                                                                              >  1:242404/1‑140 (MQ=255)

                                cccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAg                                                                                              >  1:415044/1‑140 (MQ=255)

                                 ccTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGt                                                                                             <  1:22948/140‑1 (MQ=255)

                                  cTTGCGCTACTACGCGCTCCACGTTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGt                                                                                             <  2:140225/140‑1 (MQ=255)

                                  cTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTc                                                                                            <  1:1430124/140‑1 (MQ=255)

                                    tGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCt                                                                                          >  1:793006/1‑140 (MQ=255)

                                       gcTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 >  2:561156/1‑130 (MQ=255)

                                       gcTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTcgcg                                                                                                 <  1:561156/130‑1 (MQ=255)

                                        ctactaCGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGtt                                                                                      >  1:1372324/1‑140 (MQ=255)

                                           atacGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTaaa                                                                                   <  2:1217087/139‑1 (MQ=255)

                                              cgcgCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGt                                                                                             <  1:1755311/127‑1 (MQ=255)

                                              cgcgCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGt                                                                                             >  2:1755311/1‑127 (MQ=255)

                                              cgcgCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAagag                                                                                >  1:1207203/1‑140 (MQ=255)

                                                   tCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGTAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGCTAAAGAGGTTaa                                                                           >  1:402874/1‑140 (MQ=255)

                                                     cACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAgg                                                                         >  2:1968949/1‑140 (MQ=255)

                                                        g‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAg                                                                      <  2:43814/140‑1 (MQ=255)

                                                        g‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAg                                                                      <  1:2099145/140‑1 (MQ=255)

                                                          ttttCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAgc                                                                     >  2:1786416/1‑140 (MQ=255)

                                                              cgcgGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTc                                                                 >  2:1458571/1‑140 (MQ=255)

                                                              cgcgGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTc                                                                 >  2:77726/1‑140 (MQ=255)

                                                                cgGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCaa                                                               >  2:1784766/1‑140 (MQ=255)

                                                                   gaGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAg                                                            <  1:1649255/139‑1 (MQ=255)

                                                                   cAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAg                                                            <  2:1096843/140‑1 (MQ=255)

                                                                   cAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGAAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAg                                                            <  1:1083264/140‑1 (MQ=255)

                                                                     gTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgc                                                          <  2:1925257/140‑1 (MQ=255)

                                                                      tCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcg                                                         <  2:1467325/140‑1 (MQ=255)

                                                                      tCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcg                                                         >  1:862931/1‑140 (MQ=255)

                                                                      tCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAA‑TTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  1:1183596/140‑1 (MQ=255)

                                                                       cGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  2:1508029/140‑1 (MQ=255)

                                                                       cGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  1:534568/140‑1 (MQ=255)

                                                                       cGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  1:24748/140‑1 (MQ=255)

                                                                       cGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCACGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  2:1944056/140‑1 (MQ=255)

                                                                       cGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATAAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgcgc                                                        <  2:1760447/140‑1 (MQ=255)

                                                                           cAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTa                                                    >  1:351651/1‑140 (MQ=255)

                                                                            aaTGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTAc                                                   <  2:651328/140‑1 (MQ=255)

                                                                            aaTGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTAc                                                   <  2:415044/140‑1 (MQ=255)

                                                                               gCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATa                                                <  2:281062/140‑1 (MQ=255)

                                                                               gCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATa                                                <  2:858852/140‑1 (MQ=255)

                                                                                ccTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATaa                                               >  1:590608/1‑140 (MQ=255)

                                                                                 cTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAt                                              <  1:1867730/140‑1 (MQ=255)

                                                                                 cTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAt                                              <  1:1904738/140‑1 (MQ=255)

                                                                                 cTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAATAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATa                                                <  2:1237979/138‑1 (MQ=255)

                                                                                   tAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgt                                            >  1:527006/1‑140 (MQ=255)

                                                                                    aGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgtg                                           >  1:318969/1‑140 (MQ=255)

                                                                                        aCGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTa                                       >  2:1482879/1‑140 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     <  2:1207203/140‑1 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     >  2:667330/1‑140 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     >  1:1299565/1‑140 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     >  2:481799/1‑140 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     >  2:1721947/1‑140 (MQ=255)

                                                                                          ggCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGc                                     >  2:1788227/1‑140 (MQ=255)

                                                                                           gCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCa                                    <  2:1776504/140‑1 (MQ=255)

                                                                                           gCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCa                                    <  1:1482879/140‑1 (MQ=255)

                                                                                           gCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCa                                    <  1:1746840/140‑1 (MQ=255)

                                                                                            cTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCAt                                   <  1:1462298/140‑1 (MQ=255)

                                                                                            atGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCAt                                   <  2:566138/139‑1 (MQ=255)

                                                                                             tGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATg                                  <  1:106859/140‑1 (MQ=255)

                                                                                             tGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATg                                  <  2:1945834/140‑1 (MQ=255)

                                                                                             tGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATg                                  <  2:318969/140‑1 (MQ=255)

                                                                                             tGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATg                                  <  2:2053262/140‑1 (MQ=255)

                                                                                               gAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATggg                                >  1:1470359/1‑140 (MQ=255)

                                                                                                aaCGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                               >  2:1332955/1‑140 (MQ=255)

                                                                                                aaCGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATgggg                               >  2:666335/1‑140 (MQ=255)

                                                                                                  cGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGtt                             <  2:1350470/140‑1 (MQ=255)

                                                                                                  cGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGtt                             <  2:1855201/140‑1 (MQ=255)

                                                                                                    aCATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTaa                           <  2:590608/140‑1 (MQ=255)

                                                                                                    aCATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTaa                           <  1:1255212/140‑1 (MQ=255)

                                                                                                       tGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAgtg                        <  1:1784766/140‑1 (MQ=255)

                                                                                                          tCCGGGAGAATTTTAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAg                     >  1:1915307/1‑140 (MQ=255)

                                                                                                                    ttttAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCaa           >  2:762628/1‑140 (MQ=255)

                                                                                                                     tttAGAAAACGGAGGCAATTAAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAAt          <  1:2080327/140‑1 (MQ=255)

GGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACG‑TTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTCAATGAGAAACG  >  NC_000913/3645019‑3645282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A