Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,411	G→T	G361W (GGG→TGG)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,411	0	G	T	100.0%	260.5 / NA	76	G361W (GGG→TGG)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (45/31); total (45/31)

CGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGC  >  NC_000913/3956275‑3956545

cGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTtggctggc                                                                                                                                      <  2:1455486/140‑1 (MQ=255)

  gTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCACCTGCTTGGCTGGCGt                                                                                                                                    <  2:1800696/140‑1 (MQ=255)

  gTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGt                                                                                                                                    >  1:1816346/1‑140 (MQ=255)

   tGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGtt                                                                                                                                   >  1:1433659/1‑140 (MQ=255)

   tGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGtt                                                                                                                                   <  2:1455709/140‑1 (MQ=255)

         cGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCa                                                                                                                             <  1:1595244/140‑1 (MQ=255)

             aaCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGa                                                                                                                         <  2:126019/140‑1 (MQ=255)

             aaCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGa                                                                                                                         >  1:1641018/1‑140 (MQ=255)

              aCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAg                                                                                                                        <  2:1888644/140‑1 (MQ=255)

                  ccACGGCCTGCGCTACGTCTCAGAACGCTGCGAACCGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGTAAGAAAAAGGCAGCCTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGACTTCa                                                                                                                    >  2:1602514/1‑140 (MQ=255)

                      ggCCTGCGCTACGTCTCAGAACGCTGCGAACTTGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTa                                                                                                                <  2:1806617/140‑1 (MQ=255)

                       gCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTAc                                                                                                               >  1:1805456/1‑140 (MQ=255)

                          tGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGt                                                                                                            >  2:974505/1‑140 (MQ=255)

                          tGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGt                                                                                                            >  2:507552/1‑140 (MQ=255)

                            cgcTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGttt                                                                                                          >  2:1021178/1‑140 (MQ=255)

                            cgcTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGttt                                                                                                          >  1:1942435/1‑140 (MQ=255)

                                aCGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGcc                                                                                                      <  1:852467/140‑1 (MQ=255)

                                 agTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCg                                                                                                     <  2:1805456/139‑1 (MQ=255)

                                         aaCGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑aaa                                                                                            >  1:11804/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:1747469/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:1196829/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:720530/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:1942920/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  1:244428/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:1987164/1‑140 (MQ=255)

                                              tGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGc                                                                                       >  2:2057699/1‑140 (MQ=255)

                                                 gAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCtt                                                                                    >  2:636041/1‑139 (MQ=255)

                                                   acttggCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCa                                                                                  >  2:969796/5‑140 (MQ=255)

                                                   aCTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCa                                                                                  <  1:1147765/140‑1 (MQ=255)

                                                    cTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCAt                                                                                 <  1:1942920/140‑1 (MQ=255)

                                                    cTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCAt                                                                                 <  1:1196829/140‑1 (MQ=255)

                                                          gAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGt                                                                           <  1:720530/140‑1 (MQ=255)

                                                           aaCAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTc                                                                          >  1:1581732/1‑140 (MQ=255)

                                                            aCAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCCACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCg                                                                         >  2:2068065/1‑140 (MQ=255)

                                                               gCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGgtg                                                                      <  1:974505/140‑1 (MQ=255)

                                                                 gTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGtgtg                                                                    >  1:124933/1‑140 (MQ=255)

                                                                        gttgttGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCAATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGa                                                             >  2:398377/1‑140 (MQ=255)

                                                                          tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGt                                                                           >  1:1627778/1‑124 (MQ=255)

                                                                          tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGt                                                                           <  2:1627778/124‑1 (MQ=255)

                                                                          tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGc                                                           >  1:2136640/1‑140 (MQ=255)

                                                                          tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGc                                                           >  2:1844195/1‑140 (MQ=255)

                                                                            ttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCcg                                                         >  1:833026/1‑140 (MQ=255)

                                                                            ttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCcg                                                         <  1:969796/140‑1 (MQ=255)

                                                                            ttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCcg                                                         <  2:833026/140‑1 (MQ=255)

                                                                              ggCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCcgcg                                                       <  2:1581851/140‑1 (MQ=255)

                                                                                 ggTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGcc                                                    >  2:279537/1‑140 (MQ=255)

                                                                                   tGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTc                                                  >  1:609995/1‑140 (MQ=255)

                                                                                   tGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTc                                                  >  2:1676483/1‑140 (MQ=255)

                                                                                    gACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCg                                                 >  1:359367/1‑140 (MQ=255)

                                                                                    gACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCg                                                 >  2:1445401/1‑140 (MQ=255)

                                                                                      ccATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGaa                                               >  2:462834/1‑140 (MQ=255)

                                                                                      ccATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGaa                                               >  2:2077247/1‑140 (MQ=255)

                                                                                         ttCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAaga                                             >  1:1469637/1‑140 (MQ=255)

                                                                                         ttCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAagag                                            >  2:383106/1‑140 (MQ=255)

                                                                                         ttCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAagag                                            <  2:244428/140‑1 (MQ=255)

                                                                                           ccGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAgcg                                          >  1:1690320/1‑140 (MQ=255)

                                                                                                agaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAg                                     <  2:1641018/140‑1 (MQ=255)

                                                                                                agaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAg                                     <  1:462834/140‑1 (MQ=255)

                                                                                                agaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAg                                     <  2:609995/140‑1 (MQ=255)

                                                                                                 gaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGa                                    >  1:747559/1‑140 (MQ=255)

                                                                                                   aaaaGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGaaa                                  >  1:1936546/1‑140 (MQ=255)

                                                                                                      aGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAAttt                               <  2:1690320/140‑1 (MQ=255)

                                                                                                         cAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGc                            <  1:507552/140‑1 (MQ=255)

                                                                                                         cAGCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGc                            <  2:359367/140‑1 (MQ=255)

                                                                                                         cAGCTTCCT‑‑AATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAg                          >  1:1459853/1‑140 (MQ=255)

                                                                                                           gCTTCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAg                          <  1:1858311/140‑1 (MQ=255)

                                                                                                              tCCTCAAATTCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATg                       <  1:1445401/140‑1 (MQ=255)

                                                                                                                      ttCTGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAacga               >  1:144549/1‑140 (MQ=255)

                                                                                                                         tGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcgg            >  2:1117000/1‑140 (MQ=255)

                                                                                                                         tGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcgg            <  1:1676483/140‑1 (MQ=255)

                                                                                                                         tGCCAACTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcgg            <  1:1747469/140‑1 (MQ=255)

                                                                                                                             aaCTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACggcggc        <  2:1459853/140‑1 (MQ=255)

                                                                                                                               cTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTa      >  2:523384/1‑140 (MQ=255)

                                                                                                                               cTGCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTa      >  2:956375/1‑140 (MQ=255)

                                                                                                                               atGCTTGGATGACGTTCGGTCACCGAGTTCAACTACCGTTTTGACGATGCC‑AAAAACGCCAGCATCTTTGTCGGTGTGCACCTGAGCCGCGGCATCGAAGAGCGCAAAGAAATTATGCAGATGCTAAACGACGGCGGCTa      <  2:451793/139‑1 (MQ=255)

                                                                                                                                 gCTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACa    <  1:2077247/140‑1 (MQ=255)

                                                                                                                                  cTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAg   <  1:636041/140‑1 (MQ=255)

                                                                                                                                  cTTGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAg   >  1:1513194/1‑140 (MQ=255)

                                                                                                                                   ttGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAg   <  2:1469637/140‑1 (MQ=255)

                                                                                                                                   ttGGCTGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGc  >  2:1355789/1‑140 (MQ=255)

CGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCC‑AAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGC  >  NC_000913/3956275‑3956545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A