Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,047,707	C→A	Q248K (CAG→AAG)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,047,707	0	C	A	100.0%	195.9 / NA	58	Q248K (CAG→AAG)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (24/34); total (24/34)

AGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACCAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAG  >  NC_000913/4047570‑4047842

aGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAg                                                                                                                                       <  1:1227690/140‑1 (MQ=255)

      tgctgcAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGcg                                                                                                                                 <  1:1561407/140‑1 (MQ=255)

           aaaGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGAt                                                                                                                            <  2:1178097/139‑1 (MQ=255)

            aaGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGAtt                                                                                                                           >  2:924225/1‑140 (MQ=255)

            aaGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGAtt                                                                                                                           >  2:1769524/1‑140 (MQ=255)

                tCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTaaaa                                                                                                                       >  1:1124346/1‑140 (MQ=255)

                 cTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAAc                                                                                                                      <  2:628538/140‑1 (MQ=255)

                 cTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATATCTCATACAAGCTGGCGACGATTAAAAc                                                                                                                      <  1:1533919/140‑1 (MQ=255)

                   tGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCg                                                                                                                    <  1:1769524/140‑1 (MQ=255)

                   tGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCg                                                                                                                    <  1:1610256/140‑1 (MQ=255)

                       ggACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGt                                                                                                                <  2:566711/140‑1 (MQ=255)

                          cTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGa                                                                                                             >  1:2032310/1‑140 (MQ=255)

                            ggATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAAc                                                                                                           <  2:2013138/140‑1 (MQ=255)

                                  gCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGc                                                                                                     >  1:103132/1‑140 (MQ=255)

                                   cTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCt                                                                                                    <  1:55045/140‑1 (MQ=255)

                                   cTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCt                                                                                                    >  1:292123/1‑140 (MQ=255)

                                     gTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGa                                                                                                  >  1:174359/1‑140 (MQ=255)

                                      tATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAc                                                                                                 <  1:1624068/140‑1 (MQ=255)

                                      tATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAc                                                                                                 <  1:786978/140‑1 (MQ=255)

                                      tATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAc                                                                                                 >  1:1155574/1‑140 (MQ=255)

                                      tATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAc                                                                                                 <  2:212526/140‑1 (MQ=255)

                                       aTGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAcc                                                                                                >  1:2063378/1‑140 (MQ=255)

                                       aTGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAcc                                                                                                >  1:387620/1‑140 (MQ=255)

                                       aTGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGAcc                                                                                                >  2:325514/1‑140 (MQ=255)

                                         gCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCtg                                                                                              <  2:174359/140‑1 (MQ=255)

                                           cGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCtgtg                                                                                            <  2:1124346/140‑1 (MQ=255)

                                            gAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGa                                                                                           >  1:1915965/1‑140 (MQ=255)

                                            gAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGa                                                                                           <  2:260372/140‑1 (MQ=255)

                                            gAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGa                                                                                           <  2:2032310/140‑1 (MQ=255)

                                                  gAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACt                                                                                     >  1:1320323/1‑140 (MQ=255)

                                                    aaaaaTTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTgg                                                                                   <  1:1647812/140‑1 (MQ=255)

                                                                   gAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCgg                                                                    <  2:103132/140‑1 (MQ=255)

                                                                      cTTCCATGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAg                                                                 <  2:1320323/140‑1 (MQ=255)

                                                                       ttCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGc                                                                <  2:1725959/140‑1 (MQ=255)

                                                                         ccGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCgg                                                              >  1:1526034/1‑140 (MQ=255)

                                                                         ccGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCgg                                                              >  1:1562795/1‑140 (MQ=255)

                                                                            tGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAag                                                           <  2:387620/140‑1 (MQ=255)

                                                                             ggCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAaga                                                          <  2:1547148/140‑1 (MQ=255)

                                                                                        aaTGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGc                                               <  2:292123/140‑1 (MQ=255)

                                                                                        aaTGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGc                                               <  2:2063378/140‑1 (MQ=255)

                                                                                            gCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGtt                                           <  2:1915965/140‑1 (MQ=255)

                                                                                             cAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCATCGGAAGAGTTGTTGGGGCTGTTc                                          >  2:1278184/1‑140 (MQ=255)

                                                                                             cAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTc                                          >  1:101093/1‑140 (MQ=255)

                                                                                              aGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCa                                         >  1:1070937/1‑140 (MQ=255)

                                                                                                cGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCaaa                                       >  1:1892272/1‑140 (MQ=255)

                                                                                                    gCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGt                                   <  2:1945159/140‑1 (MQ=255)

                                                                                                     cTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTa                                  <  2:1627967/140‑1 (MQ=255)

                                                                                                      tCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTAt                                 <  2:101093/140‑1 (MQ=255)

                                                                                                              aaCAAAGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCaa                         >  1:406458/1‑140 (MQ=255)

                                                                                                                 aaaGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACg                      <  2:1816075/140‑1 (MQ=255)

                                                                                                                 aaaGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACg                      <  1:1345186/140‑1 (MQ=255)

                                                                                                                 aaaGAAGTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACg                      <  2:1155574/140‑1 (MQ=255)

                                                                                                                     aagTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTgg                  <  2:1562795/140‑1 (MQ=255)

                                                                                                                       gTTGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGAc                <  1:543549/140‑1 (MQ=255)

                                                                                                                        ttGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGAct               >  1:155984/1‑140 (MQ=255)

                                                                                                                        ttGCTTATCTCTCATACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGAct               >  2:446882/1‑140 (MQ=255)

                                                                                                                                     aTACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAg  >  1:1567784/1‑140 (MQ=255)

                                                                                                                                     aTACAAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAg  >  1:1658388/1‑140 (MQ=255)

AGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATACCAGCTGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAG  >  NC_000913/4047570‑4047842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A