Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,133,316	G→T	W234L (TGG→TTG)	metF →	5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,133,316	0	G	T	100.0%	308.9 / NA	90	W234L (TGG→TTG)	metF	5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (41/49); total (41/49)

CTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGT  >  NC_000913/4133185‑4133452

cTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGgcgc                                                                                                                                  <  2:1979799/140‑1 (MQ=255)

   ccTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGGAAATATTTCGGGGATTTTTCCGGTATCTAACTTTAAAAAAGCGGAAAAAATTTCCGCTATGACCAACGCGCGTATTTCAGCGTTGAGGGCGCcaa                                                                                                                               >  2:338948/1‑140 (MQ=255)

         ttttCGTGACCGCTGTGTATCGGCGGGCATTTATGT‑GGGAATTATTTCGGGAATTTTTCCGGGATCTAACTTTAAACAGGCGAAGAAATTTTCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAAAGTTCg                                                                                                                         >  2:1266914/1‑140 (MQ=255)

         ttttCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCg                                                                                                                         <  1:397498/140‑1 (MQ=255)

          tttCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGGAAATATTTCGGGGATTTTTCCGGTGTCTAACTTTTAACAAGCGAAGAAAATTTTCGATATGAACAACCTGCGTATTTCTGCGGTGATGGGGCAAAAGtttgt                                                                                                                        >  2:1002090/1‑137 (MQ=255)

          tttCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGa                                                                                                                        <  1:1642561/140‑1 (MQ=255)

          tttCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGa                                                                                                                        <  1:1564101/140‑1 (MQ=255)

          ttgcatGACCGCTGTGATGCGGGGGGCATTGGTGT‑GGGAATTATTCCGGGGATTTTGCCGGTATGTATCTTTAAAGAGGCGAAGAAATTTGCCGATATGGCCAACGTGCGTATTCCGGGGTTGATGGCGCAAATGTTCGa                                                                                                                        <  2:1162729/135‑1 (MQ=255)

           ttCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGAc                                                                                                                       <  1:1767787/140‑1 (MQ=255)

             cGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACgg                                                                                                                     <  1:750065/140‑1 (MQ=255)

              gTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGt                                                                                                                    <  1:1804542/140‑1 (MQ=255)

              gTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGt                                                                                                                    <  1:1790933/140‑1 (MQ=255)

              gTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGt                                                                                                                    <  2:831966/140‑1 (MQ=255)

              gTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGt                                                                                                                    <  1:1002090/140‑1 (MQ=255)

                 gccGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTg                                                                                                                 <  1:178353/139‑1 (MQ=255)

                   cGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGga                                                                                                               <  1:366521/140‑1 (MQ=255)

                               gcggGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGa                                                                                                   <  2:1079199/140‑1 (MQ=255)

                               gcggGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGa                                                                                                   <  1:1238644/140‑1 (MQ=255)

                               gcggGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGa                                                                                                   <  1:170901/140‑1 (MQ=255)

                                  ggCATTGATGTGGGAAATATTTCGGGGAATTTTG‑CGGGATCTAACTTTAAAAAAGGGAAGAAAATTTCCGATATGACCAACGTGCGTATTTCGGCGTTGATGGCGGAAATGTTCGACGGTCTTGGTGATGATGCCGaaaa                                                                                                >  2:1238644/1‑139 (MQ=255)

                                    gaTTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAAccc                                                                                              <  1:1038771/139‑1 (MQ=255)

                                         aTGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCaaa                                                                                         >  2:99077/1‑140 (MQ=255)

                                         aTGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCaaa                                                                                         <  1:1903571/140‑1 (MQ=255)

                                         aTGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCaaa                                                                                         <  1:59333/140‑1 (MQ=255)

                                                aaaTTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGtt                                                                                   <  1:2072350/140‑1 (MQ=255)

                                                  attattCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTgg                                                                                 >  2:1989779/1‑140 (MQ=255)

                                                  attattCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTgg                                                                                 >  2:1040744/1‑140 (MQ=255)

                                                   ttattCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGgc                                                                                <  2:58968/140‑1 (MQ=255)

                                                    tattCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGgcg                                                                               >  1:1824222/1‑140 (MQ=255)

                                                          gggAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTTGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAata                                                                         <  1:1314373/140‑1 (MQ=255)

                                                          gggAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAata                                                                         <  1:126589/140‑1 (MQ=255)

                                                          gggAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAata                                                                         <  1:219627/140‑1 (MQ=255)

                                                          gggAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAata                                                                         <  1:338948/140‑1 (MQ=255)

                                                            gAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATAtt                                                                       <  1:2005400/140‑1 (MQ=255)

                                                              aTTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGc                                                                     <  1:1984115/140‑1 (MQ=255)

                                                              aTTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGc                                                                     <  1:1063322/140‑1 (MQ=255)

                                                               ttttGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGcc                                                                    <  1:918077/140‑1 (MQ=255)

                                                               ttttGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGcc                                                                    <  1:212085/140‑1 (MQ=255)

                                                               ttttGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGcc                                                                    <  1:288463/140‑1 (MQ=255)

                                                                tttGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGcct                                                                   >  2:1213738/1‑139 (MQ=255)

                                                                         aTCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATgg                                                          >  2:17376/1‑140 (MQ=255)

                                                                            tAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGa                                                       <  1:99077/140‑1 (MQ=255)

                                                                            tAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGa                                                       <  2:1824222/140‑1 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGCTGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  2:1184086/1‑140 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGa                                                       >  2:507156/1‑134 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGa                                                       <  1:507156/134‑1 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  1:1876821/1‑140 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  2:187296/1‑140 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  2:756074/1‑140 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  1:1997389/1‑140 (MQ=255)

                                                                                  tAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAttt                                                 >  1:205399/1‑140 (MQ=255)

                                                                                    aaCAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTa                                               >  2:1811408/1‑140 (MQ=255)

                                                                                    aaCAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTa                                               >  2:1020070/1‑140 (MQ=255)

                                                                                    aaCAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTa                                               >  1:1058631/1‑140 (MQ=255)

                                                                                        ggCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGcc                                           >  2:309190/1‑140 (MQ=255)

                                                                                         gCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGcct                                          >  2:542138/1‑139 (MQ=255)

                                                                                         gCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCg                                          >  2:1985206/1‑140 (MQ=255)

                                                                                         gCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCg                                          >  1:1820602/1‑140 (MQ=255)

                                                                                         gCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCg                                          >  2:1524395/1‑140 (MQ=255)

                                                                                          cGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGTGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGt                                         >  2:235606/1‑140 (MQ=255)

                                                                                          cGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGTAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGt                                         >  1:1688939/1‑140 (MQ=255)

                                                                                          cGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCTCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGt                                         >  2:2026554/1‑140 (MQ=255)

                                                                                          cGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGt                                         >  1:1579829/1‑140 (MQ=255)

                                                                                          cGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGt                                         >  2:314960/1‑140 (MQ=255)

                                                                                            aagaaATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGa                                       <  1:634143/140‑1 (MQ=255)

                                                                                             agaaATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGaa                                      <  2:1997389/140‑1 (MQ=255)

                                                                                              gaaATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAg                                     <  2:1579829/140‑1 (MQ=255)

                                                                                              gaaATTTGCCGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAg                                     <  2:1058631/140‑1 (MQ=255)

                                                                                                       cGATATGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAg                            <  1:187296/140‑1 (MQ=255)

                                                                                                           atGACCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGAttt                        >  1:53498/1‑140 (MQ=255)

                                                                                                              aCCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCa                     <  1:309190/140‑1 (MQ=255)

                                                                                                              aCCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCa                     <  1:452967/140‑1 (MQ=255)

                                                                                                              aCCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCa                     <  2:1502122/140‑1 (MQ=255)

                                                                                                              aCCAACGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCa                     <  2:2073714/140‑1 (MQ=255)

                                                                                                                   cGTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCt                >  2:1689078/1‑140 (MQ=255)

                                                                                                                    gTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCta               <  1:2114720/140‑1 (MQ=255)

                                                                                                                    gTGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCta               >  1:1482831/1‑140 (MQ=255)

                                                                                                                     tGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCtat              >  2:844515/1‑140 (MQ=255)

                                                                                                                     tGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCt                >  1:881052/1‑138 (MQ=255)

                                                                                                                     tGCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCt                <  2:881052/138‑1 (MQ=255)

                                                                                                                      gCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCtata             >  2:1325129/1‑140 (MQ=255)

                                                                                                                      gCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCt                <  1:2151911/137‑1 (MQ=255)

                                                                                                                      gCGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCt                >  2:2151911/1‑137 (MQ=255)

                                                                                                                       cGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATAc            >  1:1237387/1‑140 (MQ=255)

                                                                                                                       cGTATTCCGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATAc            >  2:1427218/1‑140 (MQ=255)

                                                                                                                             ccGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTaa      >  2:206600/1‑140 (MQ=255)

                                                                                                                             ccGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTaa      <  1:542138/140‑1 (MQ=255)

                                                                                                                             ccGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAACCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTaa      <  2:1876821/140‑1 (MQ=255)

                                                                                                                              cGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATTATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc     >  2:1280155/1‑140 (MQ=255)

                                                                                                                              cGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc     >  1:1092947/1‑140 (MQ=255)

                                                                                                                              cGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc     >  1:1954447/1‑140 (MQ=255)

                                                                                                                              cGGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc     >  2:1522343/1‑140 (MQ=255)

                                                                                                                               ggCGTTGATGGCTCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTTTACGCTTAAcc    <  1:1268537/140‑1 (MQ=255)

                                                                                                                                 cGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGt  >  1:1547130/1‑140 (MQ=255)

CTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT‑GGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGT  >  NC_000913/4133185‑4133452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A