Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,138,141	G→T	L299I (CTT→ATT)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,138,141	0	G	T	100.0%	422.5 / NA	122	L299I (CTT→ATT)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (69/53); total (69/53)

Only 100 of 126 total aligned reads displayed.

GTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCG  >  NC_000913/4138005‑4138274

gTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAg                                                                                                                                       >  1:456274/1‑137 (MQ=255)

gTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAg                                                                                                                                       <  2:456274/137‑1 (MQ=255)

    cgccgcCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCt                                                                                                                                >  2:1398600/1‑140 (MQ=255)

        gccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACgg                                                                                                                            >  1:1658368/1‑140 (MQ=255)

         ccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGt                                                                                                                           >  2:486884/1‑140 (MQ=255)

             gCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCg                                                                                                                       <  2:2149795/140‑1 (MQ=255)

                   tgtgAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTtcc                                                                                                                 >  2:1685852/1‑140 (MQ=255)

                     tgAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTtcctc                                                                                                               >  1:457074/1‑140 (MQ=255)

                       aaTGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCa                                                                                                             >  2:125869/1‑140 (MQ=255)

                            tttCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGgc                                                                                                        >  2:1260570/1‑140 (MQ=255)

                             ttCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGgcg                                                                                                       <  2:1902929/140‑1 (MQ=255)

                              tCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGgcgc                                                                                                      >  2:1538536/1‑140 (MQ=255)

                              tCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGgcgc                                                                                                      >  1:500854/1‑140 (MQ=255)

                               cACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCa                                                                                                     <  1:162759/140‑1 (MQ=255)

                                aCCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCAt                                                                                                    <  1:1871738/140‑1 (MQ=255)

                                   ttcttcACATTCCGTTTCTGCCTCAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGATAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCAtttt                                                                                                 <  2:1160207/140‑1 (MQ=255)

                                    tCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTc                                                                                                >  1:1469460/1‑140 (MQ=255)

                                    tCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTc                                                                                                <  1:344825/140‑1 (MQ=255)

                                     cTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTcc                                                                                               >  1:586396/1‑140 (MQ=255)

                                      tGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCa                                                                                              >  1:1075573/1‑140 (MQ=255)

                                         acaTGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGaa                                                                                           <  1:585670/140‑1 (MQ=255)

                                          caTGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAAc                                                                                          >  1:492375/1‑140 (MQ=255)

                                           aTGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAAcc                                                                                         >  1:1502187/1‑140 (MQ=255)

                                            tGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCa                                                                                        >  2:134731/1‑140 (MQ=255)

                                            tGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCa                                                                                        >  2:904044/1‑140 (MQ=255)

                                            tGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCa                                                                                        >  2:324766/1‑140 (MQ=255)

                                            tGCCACTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCa                                                                                        >  2:1374942/1‑140 (MQ=255)

                                             gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCac                                                                                       >  2:1676699/1‑139 (MQ=255)

                                             gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAg                                                                                       >  2:1938309/1‑140 (MQ=255)

                                             gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAg                                                                                       >  1:786135/1‑140 (MQ=255)

                                             gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAg                                                                                       >  2:1273066/1‑140 (MQ=255)

                                             gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAg                                                                                       >  2:1417678/1‑140 (MQ=255)

                                                gCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTg                                                                                    >  1:295043/1‑140 (MQ=255)

                                                  ttCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCg                                                                                  >  1:1784994/1‑140 (MQ=255)

                                                  ttCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCg                                                                                  >  2:1455039/1‑140 (MQ=255)

                                                  ttCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCg                                                                                  >  2:522313/1‑140 (MQ=255)

                                                  ttCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCg                                                                                  <  2:1502187/140‑1 (MQ=255)

                                                   tCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGt                                                                                 <  1:125869/140‑1 (MQ=255)

                                                   tCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGt                                                                                 >  2:622041/1‑140 (MQ=255)

                                                   tCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGAATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGt                                                                                 <  2:1075573/140‑1 (MQ=255)

                                                                  aTTTTCGCCGGGACATCTTCTTTCATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGc                                                                  <  2:1815242/140‑1 (MQ=255)

                                                                    tttCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCa                                                                <  1:945398/140‑1 (MQ=255)

                                                                    tttCGCCGGAACATCTGCTTTAATAACCAGTTGAGCGAGAGTTATTGGCAGTCCTGCCGCATGGATAATGGCAGCGACGGTGTCGATGTCCGCCACCGTCGCATTTACCAGACCCAGCTGCGTCAGCGTACCAAATACCa                                                                <  2:1533183/140‑1 (MQ=255)

                                                                           gggACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTc                                                         <  2:1658368/140‑1 (MQ=255)

                                                                           gggACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTc                                                         <  1:1273066/140‑1 (MQ=255)

                                                                           gggACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTc                                                         <  2:786135/140‑1 (MQ=255)

                                                                            ggACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCa                                                        >  1:1050235/1‑140 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  1:1455039/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  2:1546381/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  2:1678380/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  1:1685852/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  2:833862/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  2:1050235/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  1:595446/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  1:622041/140‑1 (MQ=255)

                                                                             gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                                       <  2:457074/140‑1 (MQ=255)

                                                                              aCATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAcc                                                      <  1:473508/140‑1 (MQ=255)

                                                                                aTCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGTCACTTTTTCACCGt                                                    <  1:502894/140‑1 (MQ=255)

                                                                                aTCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGt                                                    >  2:796514/1‑140 (MQ=255)

                                                                                aTCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGt                                                    <  2:500854/140‑1 (MQ=255)

                                                                                      tttAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGataat                                              >  2:675887/1‑140 (MQ=255)

                                                                                        tAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAg                                            <  1:134731/140‑1 (MQ=255)

                                                                                         aaTATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGt                                           <  2:492375/140‑1 (MQ=255)

                                                                                          atatCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTg                                          >  1:1769036/1‑140 (MQ=255)

                                                                                           tatCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGt                                                    >  2:2011555/1‑129 (MQ=255)

                                                                                           tatCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGt                                                    <  1:2011555/129‑1 (MQ=255)

                                                                                           tatCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGa                                         >  1:2046750/1‑140 (MQ=255)

                                                                                           tatCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGa                                         >  2:1834383/1‑140 (MQ=255)

                                                                                             tCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATg                                       >  1:719958/1‑140 (MQ=255)

                                                                                             tCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATg                                       >  1:665895/1‑140 (MQ=255)

                                                                                                aGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                                    >  1:1899668/1‑140 (MQ=255)

                                                                                                aGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                                    >  1:2115555/1‑140 (MQ=255)

                                                                                                aGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                                    >  1:415412/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  1:1776734/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  1:1698701/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  1:1111997/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  1:1143991/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  1:434187/1‑140 (MQ=255)

                                                                                                  ttGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                                  >  2:1234303/1‑140 (MQ=255)

                                                                                                   tGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTc                                 <  2:665895/140‑1 (MQ=255)

                                                                                                       cGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTTCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCggg                             >  1:254350/1‑140 (MQ=255)

                                                                                                       cGAGAGTTATTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATGATAGTGATGCGCGTCCggg                             <  1:796514/140‑1 (MQ=255)

                                                                                                               aTTGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGt                     >  1:993667/1‑140 (MQ=255)

                                                                                                                 tGGCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCa                   >  1:667157/1‑140 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  1:1374942/140‑1 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  1:1234303/140‑1 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  2:1769036/140‑1 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  1:1834383/140‑1 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  1:1034458/140‑1 (MQ=255)

                                                                                                                   gCAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAgg                 <  2:909831/140‑1 (MQ=255)

                                                                                                                    cAAACCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGc                >  2:1892805/1‑140 (MQ=255)

                                                                                                                     aaaCCTACCGCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGcc               >  2:746276/1‑140 (MQ=255)

                                                                                                                              gCATGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCAc      >  1:529224/1‑140 (MQ=255)

                                                                                                                                 tGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgc   >  1:139376/1‑140 (MQ=255)

                                                                                                                                 tGGCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgc   >  2:730303/1‑140 (MQ=255)

                                                                                                                                  ggCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgcg  >  1:1530842/1‑140 (MQ=255)

                                                                                                                                  ggCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgcg  >  1:41099/1‑140 (MQ=255)

                                                                                                                                  ggCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgcg  >  1:1065448/1‑140 (MQ=255)

                                                                                                                                  ggCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgcg  >  1:483913/1‑140 (MQ=255)

                                                                                                                                  ggCTAATGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACtgcg  >  2:865826/1‑140 (MQ=255)

GTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCG  >  NC_000913/4138005‑4138274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A