Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,150,741	C→A	L786L (CTG→CTT)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,150,741	0	C	A	100.0%	315.7 / NA	91	L786L (CTG→CTT)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (38/53); total (38/53)

GTAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACCAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGAC  >  NC_000913/4150605‑4150875

gTAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAgg                                                                                                                                     >  2:224149/1‑140 (MQ=255)

 tAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGc                                                                                                                                    <  2:1401250/140‑1 (MQ=255)

 tAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGc                                                                                                                                    <  1:622073/140‑1 (MQ=255)

 tAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGc                                                                                                                                    <  1:322414/140‑1 (MQ=255)

  aGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCg                                                                                                                                   <  1:707433/140‑1 (MQ=255)

   gCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGt                                                                                                                                  >  1:284332/1‑140 (MQ=255)

   gCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGt                                                                                                                                  <  1:1621690/140‑1 (MQ=255)

    cTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGtt                                                                                                                                 <  1:1045507/140‑1 (MQ=255)

    cTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGtt                                                                                                                                 <  1:975318/140‑1 (MQ=255)

      aaaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTgg                                                                                                                               <  2:65598/139‑1 (MQ=255)

      aaaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTgg                                                                                                                               <  2:816648/139‑1 (MQ=255)

       aaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTgg                                                                                                                               >  1:1538453/1‑139 (MQ=255)

       aaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTgg                                                                                                                               <  2:1538453/139‑1 (MQ=255)

       aaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGt                                                                                                                              <  1:171440/140‑1 (MQ=255)

       aaTAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGt                                                                                                                              <  1:369104/140‑1 (MQ=255)

         aaGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCa                                                                                                                            <  2:1078183/139‑1 (MQ=255)

          aGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCAt                                                                                                                           <  1:425216/140‑1 (MQ=255)

              tctGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTa                                                                                                                       >  2:1326921/1‑140 (MQ=255)

               ctGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTAt                                                                                                                      >  1:2036294/1‑140 (MQ=255)

                tGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTAtt                                                                                                                     >  1:1136946/1‑140 (MQ=255)

                 gCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTGTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTc                                                                                                                    >  2:1791743/1‑140 (MQ=255)

                 gCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTc                                                                                                                    >  2:562732/1‑140 (MQ=255)

                 gCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTc                                                                                                                    >  2:1669022/1‑140 (MQ=255)

                  cAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCg                                                                                                                                   <  2:2120105/124‑1 (MQ=255)

                  cAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCg                                                                                                                                   >  1:2120105/1‑124 (MQ=255)

                     tCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCTCCTCTTTGATGTCTTCTTCTTGCAGGTTGCGTACCTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgcc                                                                                                                <  1:393359/140‑1 (MQ=255)

                     tCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgcc                                                                                                                <  2:2036294/140‑1 (MQ=255)

                     tCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgcc                                                                                                                >  2:793137/1‑140 (MQ=255)

                     tCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgcc                                                                                                                <  1:849213/140‑1 (MQ=255)

                     accACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgcc                                                                                                                <  2:1136946/139‑1 (MQ=255)

                       cACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgccag                                                                                                              >  1:1312605/1‑140 (MQ=255)

                        aCGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgccagc                                                                                                             >  1:403550/1‑140 (MQ=255)

                          ggCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgccagcca                                                                                                           <  1:1423339/140‑1 (MQ=255)

                          ggCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCgccagcca                                                                                                           >  2:1957791/1‑140 (MQ=255)

                             aGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAg                                                                                                        <  1:793137/140‑1 (MQ=255)

                               aTCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGt                                                                                                      <  2:658382/140‑1 (MQ=255)

                                 cGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCt                                                                                                    >  2:876602/1‑140 (MQ=255)

                                    ccATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCt                                                                                                 <  2:419234/140‑1 (MQ=255)

                                          gATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCg                                                                                           <  1:2130350/140‑1 (MQ=255)

                                          aaTGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCg                                                                                           <  2:105989/139‑1 (MQ=255)

                                           aTGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGa                                                                                          >  1:1089775/1‑140 (MQ=255)

                                           aTGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGa                                                                                          <  1:449359/140‑1 (MQ=255)

                                             gggAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAg                                                                                        <  2:1089775/140‑1 (MQ=255)

                                             gggAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAg                                                                                        <  2:2121875/140‑1 (MQ=255)

                                              ggAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCACCAGCCACAGGTCTGCTTTGGCGAAGa                                                                                       <  2:136627/140‑1 (MQ=255)

                                              ggAATCGTTGGCAATCGCCAGCACCACTTTGATATCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGa                                                                                       <  1:503375/140‑1 (MQ=255)

                                               gAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGAc                                                                                      <  1:1517152/140‑1 (MQ=255)

                                                aaTCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGAcc                                                                                     <  2:874705/140‑1 (MQ=255)

                                                aaTCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGAcc                                                                                     >  2:1933158/1‑140 (MQ=255)

                                                     ttGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCAtctc                                                                                >  2:114096/1‑140 (MQ=255)

                                                       ggCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCa                                                                              >  1:490030/1‑140 (MQ=255)

                                                       ggCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCa                                                                              <  1:876602/140‑1 (MQ=255)

                                                       ggCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCa                                                                              <  2:1312605/140‑1 (MQ=255)

                                                        gCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAg                                                                             >  2:994815/1‑140 (MQ=255)

                                                         cAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGc                                                                            <  1:1416243/140‑1 (MQ=255)

                                                                  gCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCgaga                                                                   <  2:403550/140‑1 (MQ=255)

                                                                  gCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCgaga                                                                   <  1:2114462/140‑1 (MQ=255)

                                                                      cacTTTGATGTCTTCTTCTTGCAGATTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGAcgcg                                                               <  2:389812/140‑1 (MQ=255)

                                                                      aacTTTGATGGCTTCTTCTTGCAGGTTGCGTAGCTCTTTACCTAAAGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATGCCGCCAGCCACTGGGCTGCTTTGGCGAAGACCCTCTCCAGCATGCCGAGAcgcg                                                               <  2:284332/139‑1 (MQ=255)

                                                                           tGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCgag                                                          <  1:994815/140‑1 (MQ=255)

                                                                              tGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCATAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGagaag                                                       >  2:1695570/1‑140 (MQ=255)

                                                                              tGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGagaag                                                       <  2:1617276/140‑1 (MQ=255)

                                                                                  ttcttcttGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATg                                                   >  1:1036659/1‑140 (MQ=255)

                                                                                  ttcttcttGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATg                                                   >  1:342854/1‑140 (MQ=255)

                                                                                     ttcttGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGcc                                                >  2:1284110/1‑140 (MQ=255)

                                                                                        ttGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAAt                                             <  1:1957791/140‑1 (MQ=255)

                                                                                          gCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATcg                                           <  2:332079/140‑1 (MQ=255)

                                                                                           cAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATcgc                                          <  2:1620757/140‑1 (MQ=255)

                                                                                            aGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATcgcg                                         <  1:1284110/140‑1 (MQ=255)

                                                                                            aGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATcgcg                                         >  1:982889/1‑140 (MQ=255)

                                                                                             ggTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCgg                                        <  1:1695570/140‑1 (MQ=255)

                                                                                                  cGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACAt                                   >  2:375917/1‑140 (MQ=255)

                                                                                                     aaCTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGc                                <  1:114096/140‑1 (MQ=255)

                                                                                                     aaCTCTTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGc                                >  1:809009/1‑140 (MQ=255)

                                                                                                       atctTTACCTAACGGCCGCAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCt                              <  2:825039/139‑1 (MQ=255)

                                                                                                         ctTTACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTcc                            <  1:1068863/140‑1 (MQ=255)

                                                                                                            tACCTAACGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAgc                         >  2:264303/1‑140 (MQ=255)

                                                                                                                 aaCGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAgctcgct                    >  2:1978084/1‑140 (MQ=255)

                                                                                                                  aCGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAgctcgctc                   >  2:383288/1‑140 (MQ=255)

                                                                                                                  aCGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAgc                         <  2:158459/134‑1 (MQ=255)

                                                                                                                  aCGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAgc                         >  1:158459/1‑134 (MQ=255)

                                                                                                                   cGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGctct                  >  2:2125262/1‑140 (MQ=255)

                                                                                                                   cGGCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGctct                  >  1:1321332/1‑140 (MQ=255)

                                                                                                                    ggCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTg                 >  1:1717544/1‑140 (MQ=255)

                                                                                                                    ggCCACAGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTg                 >  1:1079161/1‑140 (MQ=255)

                                                                                                                        acaGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTg             <  1:1933158/140‑1 (MQ=255)

                                                                                                                        acaGTGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTg             >  2:2101089/1‑140 (MQ=255)

                                                                                                                            tGCTTTGTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGt         >  1:660890/1‑140 (MQ=255)

                                                                                                                                  gTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGa   <  2:450166/140‑1 (MQ=255)

                                                                                                                                  gTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGa   <  2:812788/140‑1 (MQ=255)

                                                                                                                                  gTCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGa   <  1:224149/140‑1 (MQ=255)

                                                                                                                                   tCTACAAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGAc  >  1:1598842/1‑140 (MQ=255)

GTAGCTGAATAGACTCTGCAATCCACGGCAGATCGGCCATCAGATGGGAATCGTTGGCAATCGCCAGCACCACTTTGATGTCTTCTTCTTGCAGGTTGCGTAACTCTTTACCTAACGGCCACAGTGCTTTGTCTACCAGGCGTTGGTCATAGTATTCCGCCAGCCACAGGTCTGCTTTGGCGAAGACCATCTCCAGCATGCCGAGACGCGTCGAGAAGAATGGCCAATCGCGGCACATAGCCTCCAGCTCGCTCTGTTTGCCGTCTTCGAC  >  NC_000913/4150605‑4150875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A