Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,194,194	G→T	P4H (CCT→CAT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,194,194	0	G	T	100.0%	376.9 / NA	109	P4H (CCT→CAT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (66/43); total (66/43)

Only 100 of 114 total aligned reads displayed.

TCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTCATC  >  NC_000913/4194059‑4194330

tCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTg                                                                                                                                      <  1:937114/140‑1 (MQ=255)

  cACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGAt                                                                                                                                    >  2:1265994/1‑140 (MQ=255)

    ccTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATAc                                                                                                                                  <  1:1722440/140‑1 (MQ=255)

      tctTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACAt                                                                                                                                <  2:701967/140‑1 (MQ=255)

       ctTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATc                                                                                                                               >  2:1878291/1‑140 (MQ=255)

        ttCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCa                                                                                                                              <  1:956529/140‑1 (MQ=255)

        ttCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCa                                                                                                                              <  2:25260/140‑1 (MQ=255)

          cATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACg                                                                                                                            <  1:1146280/140‑1 (MQ=255)

          cATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACg                                                                                                                            >  2:1021805/1‑140 (MQ=255)

          aaTCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACg                                                                                                                            <  2:1729276/139‑1 (MQ=255)

            tCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCt                                                                                                                          <  1:1505639/140‑1 (MQ=255)

             cgcgCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCtt                                                                                                                         <  1:449824/140‑1 (MQ=255)

             cgcgCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCtt                                                                                                                         <  2:1745706/140‑1 (MQ=255)

              gcgcCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTtc                                                                                                                        >  1:635541/1‑140 (MQ=255)

               cgcCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTtcc                                                                                                                       >  2:525963/1‑140 (MQ=255)

                 ccGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCGACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTtcctc                                                                                                                     >  1:948111/1‑140 (MQ=255)

                 ccGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGCACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTtcctc                                                                                                                     >  1:1801416/1‑140 (MQ=255)

                   gATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTcctcct                                                                                                                   <  1:1544609/140‑1 (MQ=255)

                    aTCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCtt                                                                                                                  >  1:1819466/1‑140 (MQ=255)

                     acTTTGATGCGTAGCTGGAGAGTACGTAAGACTCCATCCAACAGACGTACGAAACACTGTACGCTGCCCACCACCGGGTACAGTCATGAACGAAAAGGTACAGGAAGAAAATCATGCTGATACATCACGCTTCCTCCTTa                                                                                                                 <  2:2024327/139‑1 (MQ=255)

                      cTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTAc                                                                                                                <  2:1468285/140‑1 (MQ=255)

                       tttGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACg                                                                                                               <  2:1997877/140‑1 (MQ=255)

                        ttGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGc                                                                                                              <  1:1860582/140‑1 (MQ=255)

                          gATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAg                                                                                                            <  1:1093434/140‑1 (MQ=255)

                              cGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAg                                                                                                        >  2:574247/1‑140 (MQ=255)

                              cGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAg                                                                                                        >  1:100090/1‑140 (MQ=255)

                                 aGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGAtt                                                                                                     >  1:969786/1‑140 (MQ=255)

                                   cTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACg                                                                                                               >  1:422243/1‑128 (MQ=255)

                                   cTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACg                                                                                                               <  2:422243/128‑1 (MQ=255)

                                             cgtacgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCctctt                                                                                         >  2:78531/1‑139 (MQ=255)

                                             cgtacgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCctctt                                                                                         >  2:153947/1‑139 (MQ=255)

                                             cgtacgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTg                                                                                         >  1:1163574/1‑140 (MQ=255)

                                             cgtacgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTg                                                                                         >  2:266953/1‑140 (MQ=255)

                                                acgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTgg                                                                                        <  2:551147/138‑1 (MQ=255)

                                                 cgCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCAc                                                                                     >  2:1240262/1‑140 (MQ=255)

                                                   ccTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACgg                                                                                   <  1:1562638/140‑1 (MQ=255)

                                                     tGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGaa                                                                                 <  1:525963/140‑1 (MQ=255)

                                                     tGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGaa                                                                                 >  2:452643/1‑140 (MQ=255)

                                                       cATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGTAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGGAGATTTCTCCGCCTCTGGCACGGAAGt                                                                               >  1:903753/1‑140 (MQ=255)

                                                       cATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGt                                                                               >  2:1948439/1‑140 (MQ=255)

                                                         tCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTtc                                                                             >  2:2055263/1‑140 (MQ=255)

                                                            aaCAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTcc                                                                          >  2:586001/1‑140 (MQ=255)

                                                            aaCAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTcc                                                                          >  2:217438/1‑140 (MQ=255)

                                                            aaCAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTcc                                                                          <  1:1259852/140‑1 (MQ=255)

                                                              cAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGa                                                                        >  2:784545/1‑140 (MQ=255)

                                                                   gTTCGATCCACTGTACGCTGTCCACCACCGGTTACAGTCCTGAACGAAAAGGTGCAGGAGGAAAACCATGCTGATACATCACGCTTCCCCCTTACGCAGGTAGTTTTCTCCGCCTCTGGCACGGAAGTTCTCCGACatat                                                                   <  1:2039126/140‑1 (MQ=255)

                                                                   gTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACatat                                                                   >  2:1361606/1‑140 (MQ=255)

                                                                      caaTCCATTGTACGCTGTCCCCCCCCGGGTACAGGCCTAAAGGAAAAGGTACGGGAGGAAAATAATGCTGATACATCACGCTTCCTCCTTCCGAAGGTAGTTTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCg                                                                <  2:969786/138‑1 (MQ=255)

                                                                          ccACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCAt                                                            >  1:58709/1‑140 (MQ=255)

                                                                           aaCTGTACGCGGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCAtt                                                           <  2:607649/139‑1 (MQ=255)

                                                                             cTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTcc                                                         <  1:1879608/140‑1 (MQ=255)

                                                                             cTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTcc                                                         <  1:784545/140‑1 (MQ=255)

                                                                             cTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTcc                                                         <  1:1361606/140‑1 (MQ=255)

                                                                               gTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCa                                                       <  2:1163574/140‑1 (MQ=255)

                                                                                  cGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCAttt                                                    >  2:1056459/1‑140 (MQ=255)

                                                                                   gCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTc                                                   >  1:183059/1‑140 (MQ=255)

                                                                                   gCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTc                                                   <  1:452643/140‑1 (MQ=255)

                                                                                     tGTCCACCCCCGGGTAAAGTCCTAACCGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGTTTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCaa                                                 <  1:1021805/140‑1 (MQ=255)

                                                                                     tGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCaa                                                 >  1:961681/1‑140 (MQ=255)

                                                                                     tGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCaa                                                 >  1:1620799/1‑140 (MQ=255)

                                                                                       tCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATa                                               >  2:226548/1‑140 (MQ=255)

                                                                                       tCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATa                                               >  1:1989613/1‑140 (MQ=255)

                                                                                       tCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATa                                               >  2:419013/1‑140 (MQ=255)

                                                                                       tCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATa                                               >  1:1600019/1‑140 (MQ=255)

                                                                                        ccaccaCCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAg                                              >  1:1407718/1‑140 (MQ=255)

                                                                                        ccaccaCCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAg                                              >  1:518261/1‑140 (MQ=255)

                                                                                         caccacCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGt                                             <  2:1968699/140‑1 (MQ=255)

                                                                                          accaccGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGtt                                            >  1:493004/1‑140 (MQ=255)

                                                                                          accaccGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGtt                                            >  2:1372712/1‑140 (MQ=255)

                                                                                               cGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGt                                       >  2:1477968/1‑140 (MQ=255)

                                                                                               cGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGt                                       >  2:1278052/1‑140 (MQ=255)

                                                                                                gggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTg                                      <  1:929203/140‑1 (MQ=255)

                                                                                                 ggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTgg                                     >  1:1502784/1‑140 (MQ=255)

                                                                                                 ggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTgg                                     >  2:939347/1‑140 (MQ=255)

                                                                                                 ggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTgg                                     >  1:2065694/1‑140 (MQ=255)

                                                                                                 ggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTgg                                     >  1:1333017/1‑140 (MQ=255)

                                                                                                 ggTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTgg                                     >  2:1836707/1‑140 (MQ=255)

                                                                                                    aCAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTggcgg                                  <  1:1878291/140‑1 (MQ=255)

                                                                                                     cAGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTggcggc                                 <  1:1477968/140‑1 (MQ=255)

                                                                                                      aGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGgcggcg                                <  1:1265994/140‑1 (MQ=255)

                                                                                                      aGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGgcggcg                                <  2:1989613/140‑1 (MQ=255)

                                                                                                      aGTCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGgcggcg                                >  2:1324478/1‑140 (MQ=255)

                                                                                                        tCCTGAACGAAAAGGTACAGGAGGAAAATCATGTTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTa                              >  2:2129049/1‑140 (MQ=255)

                                                                                                        tCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGgcggcg                                <  1:957361/138‑1 (MQ=255)

                                                                                                        tCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGgcggcg                                >  2:957361/1‑138 (MQ=255)

                                                                                                        tCCTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTa                              >  2:1401324/1‑140 (MQ=255)

                                                                                                         ccTGAACGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTaa                             >  2:90693/1‑140 (MQ=255)

                                                                                                             aaCGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCAc                         >  1:766274/1‑140 (MQ=255)

                                                                                                              aCGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACg                        <  1:574247/140‑1 (MQ=255)

                                                                                                              aCGAAAAGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACg                        <  1:266953/140‑1 (MQ=255)

                                                                                                                  aaaGGTACAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACt                    >  2:1677424/1‑140 (MQ=255)

                                                                                                                        aCAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTgg              <  1:1278052/140‑1 (MQ=255)

                                                                                                                        aCAGGAGAAAAATCATGTTGATACACCAGGCTCCCTCATTACGCCGGTAGTTTCCTCCGCCTCTGGCGCGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTgg              <  2:1541629/140‑1 (MQ=255)

                                                                                                                         cAGGAGGAAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGc             <  1:1401324/140‑1 (MQ=255)

                                                                                                                            gaggaAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTgt          >  2:1645451/1‑139 (MQ=255)

                                                                                                                            gaggaAAATCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGa          >  2:1776074/1‑140 (MQ=255)

                                                                                                                                 aaaTCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTc     >  1:567512/1‑140 (MQ=255)

                                                                                                                                 aaaTCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTc     >  2:1180050/1‑140 (MQ=255)

                                                                                                                                  aaTCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTCa    <  2:58709/140‑1 (MQ=255)

                                                                                                                                  aaTCATGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTCa    <  2:518261/140‑1 (MQ=255)

TCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTTGCGTGGCGGCGTAATCACGCACTTCCTGGCTGATTTTCATC  >  NC_000913/4194059‑4194330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A