Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,940	C→T	D53D (GAC→GAT)	alr →	alanine racemase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,940	0	C	T	100.0%	281.6 / NA	82	D53D (GAC→GAT)	alr	alanine racemase 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (39/43); total (39/43)

TAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCC  >  NC_000913/4265805‑4266075

tAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCt                                                                                                                                     >  1:688803/1‑140 (MQ=255)

tAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCt                                                                                                                                     <  2:542458/140‑1 (MQ=255)

aacccgccgCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCt                                                                                                                                     <  2:839601/137‑1 (MQ=255)

 aaCCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCtt                                                                                                                                    >  1:2068416/1‑140 (MQ=255)

       cgcgCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGt                                                                                                                              >  2:1485154/1‑140 (MQ=255)

        gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTa                                                                                                                             >  1:1500845/1‑140 (MQ=255)

          gcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGc                                                                                                                           <  1:627010/140‑1 (MQ=255)

           ctctGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGcc                                                                                                                          >  2:965450/1‑140 (MQ=255)

           ctctGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGcc                                                                                                                          <  1:766671/140‑1 (MQ=255)

           ctctGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGcc                                                                                                                          <  2:756723/140‑1 (MQ=255)

                    acaACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCgaa                                                                                                                 <  1:68146/140‑1 (MQ=255)

                      atccTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCgaaga                                                                                                               <  1:812748/138‑1 (MQ=255)

                      aaCCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCgaaga                                                                                                               <  2:1853933/140‑1 (MQ=255)

                      aaCCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCgaaga                                                                                                               <  2:745239/140‑1 (MQ=255)

                      aaCCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCgaaga                                                                                                               >  2:14744/1‑140 (MQ=255)

                        ccTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGaagaag                                                                                                             <  1:547694/140‑1 (MQ=255)

                        ccTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGaagaag                                                                                                             <  1:1239938/140‑1 (MQ=255)

                          tGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGct                                                                                                           <  1:1485154/140‑1 (MQ=255)

                          tGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGct                                                                                                           <  2:1851935/140‑1 (MQ=255)

                          tGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGct                                                                                                           >  1:1249217/1‑140 (MQ=255)

                             aaCGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTg                                                                                                        >  2:2063538/1‑140 (MQ=255)

                                gTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGa                                                                                                     <  1:878592/140‑1 (MQ=255)

                                 tCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGAc                                                                                                    >  2:1360261/1‑140 (MQ=255)

                                  cTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGTGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTGGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACt                                                                                                   >  2:1628732/1‑140 (MQ=255)

                                  cTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACt                                                                                                   >  2:623637/1‑140 (MQ=255)

                                   ttCGTGAACTGGCCCCTGCCAGCAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACtg                                                                                                  <  1:1800127/140‑1 (MQ=255)

                                          aCTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCgg                                                                                           >  2:896721/1‑140 (MQ=255)

                                             ggCCCGTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCggggg                                                                                        >  2:1935497/1‑140 (MQ=255)

                                              gCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCgggggg                                                                                       >  2:1603614/1‑140 (MQ=255)

                                              gCCCCTGCCAGGAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTAGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCgggggg                                                                                       >  1:1807027/1‑140 (MQ=255)

                                               ccccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGa                                                                                      <  2:1856119/140‑1 (MQ=255)

                                               ccccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGa                                                                                      <  2:688803/140‑1 (MQ=255)

                                                cccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGaa                                                                                     >  2:1862927/1‑140 (MQ=255)

                                                    gCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGATCGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCAc                                                                                 <  1:896721/140‑1 (MQ=255)

                                                    gCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCAc                                                                                 <  1:932468/140‑1 (MQ=255)

                                                    gCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCAc                                                                                 <  1:1605624/140‑1 (MQ=255)

                                                      cAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCa                                                                               >  1:1531810/1‑140 (MQ=255)

                                                      aaGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCa                                                                               <  2:341075/139‑1 (MQ=255)

                                                        gTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGCGAATCACCaaa                                                                             >  2:1344355/1‑140 (MQ=255)

                                                         tAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAAc                                                                            <  2:645007/140‑1 (MQ=255)

                                                         tAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAAc                                                                            <  2:1249217/140‑1 (MQ=255)

                                                          aaaaTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAAcc                                                                           <  1:246288/140‑1 (MQ=255)

                                                               ggTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTAc                                                                      >  2:1416696/1‑140 (MQ=255)

                                                                  tGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGt                                                                   <  1:1004983/140‑1 (MQ=255)

                                                                   gCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGtt                                                                  >  1:1663352/1‑140 (MQ=255)

                                                                     ggtggtGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTat                                                                >  2:1805614/1‑139 (MQ=255)

                                                                         gtgAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGa                                                            <  1:1416696/140‑1 (MQ=255)

                                                                                   aCGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCtttttt                                                  >  1:1156489/1‑140 (MQ=255)

                                                                                   aCGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCtttttt                                                  <  2:1531810/140‑1 (MQ=255)

                                                                                    cGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTg                                                 <  1:965450/140‑1 (MQ=255)

                                                                                     gCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGa                                                <  2:895682/140‑1 (MQ=255)

                                                                                        tATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGc                                             <  1:646369/140‑1 (MQ=255)

                                                                                               aCGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGAt                                      >  1:1338293/1‑140 (MQ=255)

                                                                                               aCGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGAt                                      >  1:185916/1‑140 (MQ=255)

                                                                                                 ggTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCt                                    >  2:1958867/1‑140 (MQ=255)

                                                                                                  gTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTg                                   <  2:1484761/140‑1 (MQ=255)

                                                                                                   tcttctTGAGACCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGc                                  >  1:425457/1‑140 (MQ=255)

                                                                                                           agaCCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCcgac                              >  2:68017/1‑136 (MQ=255)

                                                                                                           agaCCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCcgac                              <  1:68017/136‑1 (MQ=255)

                                                                                                             aCCGCGCGAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTc                        >  1:1696706/1‑140 (MQ=255)

                                                                                                               cgcgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTg                      >  2:2009213/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  1:1075773/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:1857658/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  1:1340257/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  1:2149753/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:1027543/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:1067609/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:11164/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:1230020/1‑140 (MQ=255)

                                                                                                                 cgcgAACGCTCCCCGAGGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                    >  2:1217332/1‑140 (MQ=255)

                                                                                                                  gcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcgc                   <  2:1807027/140‑1 (MQ=255)

                                                                                                                  gcgAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcgc                   <  2:1696706/140‑1 (MQ=255)

                                                                                                                    gAACGCTCCCCGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCaa                 <  1:1360261/140‑1 (MQ=255)

                                                                                                                            cccGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACTTTTTCa         <  1:1230020/140‑1 (MQ=255)

                                                                                                                            cccGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCa         >  2:1629490/1‑140 (MQ=255)

                                                                                                                              cGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTCCTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa       <  1:2063538/140‑1 (MQ=255)

                                                                                                                              cGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa       <  2:1340257/140‑1 (MQ=255)

                                                                                                                              cGATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa       <  2:1663352/140‑1 (MQ=255)

                                                                                                                              agATGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa       <  2:33686/139‑1 (MQ=255)

                                                                                                                                 tGCTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATAccg    <  2:425457/140‑1 (MQ=255)

                                                                                                                                   cTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATAccgcc  <  1:1157667/140‑1 (MQ=255)

                                                                                                                                   cTGATGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCAAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATAccgcc  <  1:1805614/140‑1 (MQ=255)

TAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCC  >  NC_000913/4265805‑4266075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A