Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,309,977 G→T A335D (GCC→GAC)  alsA ← D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,309,9770GT100.0% 325.2 / NA 95A335D (GCC→GAC) alsAD‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/47);  total (48/47)

AACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAA  >  NC_000913/4309842‑4310107
                                                                                                                                       |                                                                                                                                  
aaCAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATc                                                                                                                                <  2:2026369/140‑1 (MQ=255)
  cAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCCCGTCATCCa                                                                                                                              <  1:684659/140‑1 (MQ=255)
      cccATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAGACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAgggg                                                                                                                          <  1:1744616/140‑1 (MQ=255)
      cccATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAgggg                                                                                                                          <  1:2078293/140‑1 (MQ=255)
        cATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTg                                                                                                                        <  1:980379/140‑1 (MQ=255)
         aTCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGa                                                                                                                       <  1:76279/140‑1 (MQ=255)
         aTCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGa                                                                                                                       <  1:884200/140‑1 (MQ=255)
          tCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGGAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGGGATGTAAGCCATCCCTTTTTTCACGTCGTCCAGGGGTGaa                                                                                                                      >  1:2024400/1‑140 (MQ=255)
          tCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGaa                                                                                                                      >  1:1674774/1‑140 (MQ=255)
           cgcgCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAAc                                                                                                                     <  1:216506/140‑1 (MQ=255)
             cgcCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGt                                                                                                                   <  2:1674774/140‑1 (MQ=255)
             cgcCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGt                                                                                                                   <  1:682856/140‑1 (MQ=255)
             cgcCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGt                                                                                                                   <  1:179385/140‑1 (MQ=255)
             cgcCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGt                                                                                                                   <  1:2144782/140‑1 (MQ=255)
               ccTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTgg                                                                                                                 <  2:2122208/140‑1 (MQ=255)
                cTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGga                                                                                                                >  2:620010/1‑140 (MQ=255)
                   tataGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGgagag                                                                                                             <  1:1148020/140‑1 (MQ=255)
                        ccgccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATc                                                                                                        >  1:1257162/1‑140 (MQ=255)
                          gccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCtt                                                                                                      >  2:1307969/1‑140 (MQ=255)
                          gccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCtt                                                                                                      >  2:197786/1‑140 (MQ=255)
                          gccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCtt                                                                                                      >  2:978809/1‑140 (MQ=255)
                          gccgTCTTTCAGACTGCATCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGCGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTTAACGTGTAGAGATATCtt                                                                                                      >  1:521753/1‑140 (MQ=255)
                            cgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCtttt                                                                                                    >  2:1882942/1‑139 (MQ=255)
                            cgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCtttt                                                                                                    >  2:311969/1‑139 (MQ=255)
                              tCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGcc                                                                                                  <  1:2018911/140‑1 (MQ=255)
                                tttCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCAt                                                                                                <  1:1307969/140‑1 (MQ=255)
                                 ttCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCAtt                                                                                               <  1:1931309/140‑1 (MQ=255)
                                        tGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACgg                                                                                        >  1:1769911/1‑140 (MQ=255)
                                        tGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACgg                                                                                        >  2:486067/1‑140 (MQ=255)
                                          cGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAt                                                                                      >  1:657680/1‑140 (MQ=255)
                                          cGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAt                                                                                      >  2:1536761/1‑140 (MQ=255)
                                          cGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAt                                                                                      >  1:937759/1‑140 (MQ=255)
                                            gCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCTGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAttt                                                                                    >  1:636556/1‑140 (MQ=255)
                                                aTCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTcc                                                                                >  1:1482554/1‑140 (MQ=255)
                                                aTCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTcc                                                                                >  1:394634/1‑140 (MQ=255)
                                                  cGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGc                                                                              >  2:384186/1‑140 (MQ=255)
                                                     cATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAg                                                                           <  1:1322329/140‑1 (MQ=255)
                                                     cATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAg                                                                           <  1:430888/140‑1 (MQ=255)
                                                     cATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAg                                                                           <  1:311969/140‑1 (MQ=255)
                                                     cATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAg                                                                           <  1:620010/140‑1 (MQ=255)
                                                               gCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATc                                                                 >  1:1989971/1‑140 (MQ=255)
                                                                  gtGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGATTATCCAc                                                              <  1:820777/139‑1 (MQ=255)
                                                                  aTGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCAc                                                              <  1:1536761/140‑1 (MQ=255)
                                                                   tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACg                                                             >  2:2077319/1‑140 (MQ=255)
                                                                   tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACg                                                             >  1:1195245/1‑140 (MQ=255)
                                                                     gAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGcc                                                           <  1:1882942/140‑1 (MQ=255)
                                                                        aaGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCaaa                                                        <  2:1257162/140‑1 (MQ=255)
                                                                          gTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                      <  1:486067/140‑1 (MQ=255)
                                                                             ggggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAc                                                   <  2:1680533/140‑1 (MQ=255)
                                                                              gggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACa                                                  >  1:1288135/1‑140 (MQ=255)
                                                                               ggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                 <  1:978809/140‑1 (MQ=255)
                                                                               ggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                 <  2:1062851/140‑1 (MQ=255)
                                                                               ggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTATTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                 <  1:1710192/140‑1 (MQ=255)
                                                                                gAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAt                                                >  2:1938174/1‑140 (MQ=255)
                                                                                   aaaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtca                                             <  2:217547/140‑1 (MQ=255)
                                                                                    aaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcat                                            <  1:285149/140‑1 (MQ=255)
                                                                                     aaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatc                                           <  2:1989971/140‑1 (MQ=255)
                                                                                     aaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatc                                           <  2:1769911/140‑1 (MQ=255)
                                                                                     aaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatc                                           >  1:1053319/1‑140 (MQ=255)
                                                                                             tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                   <  2:394634/140‑1 (MQ=255)
                                                                                             tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                   >  2:1576496/1‑140 (MQ=255)
                                                                                             tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                   <  2:1596939/140‑1 (MQ=255)
                                                                                                    ggcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCg                            <  2:1288135/140‑1 (MQ=255)
                                                                                                      cTTTCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAGTCATCAGTTCAGTACGTCCGGa                          >  1:1795581/1‑140 (MQ=255)
                                                                                                        ttCAGTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                        >  2:445521/1‑140 (MQ=255)
                                                                                                            gTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCTATTATCCACGCCAAACAGATAATTAATCAGTTCAAAACGTGCGTAACCTAc                    <  2:521753/140‑1 (MQ=255)
                                                                                                            gTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                    <  1:1576496/140‑1 (MQ=255)
                                                                                                            gTGATGTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                    <  2:718677/140‑1 (MQ=255)
                                                                                                                 gTAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAgcc               >  1:41975/1‑138 (MQ=255)
                                                                                                                  tAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc              >  2:107785/1‑140 (MQ=255)
                                                                                                                  tAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc              >  1:990474/1‑140 (MQ=255)
                                                                                                                  tAAGCCATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc              >  1:414919/1‑140 (MQ=255)
                                                                                                                      ccATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          <  1:107785/140‑1 (MQ=255)
                                                                                                                      ccATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          <  1:2077319/140‑1 (MQ=255)
                                                                                                                      ccATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          <  2:1756703/140‑1 (MQ=255)
                                                                                                                      ccATCCCTTTTTTCAAGTCATCACGGGGTGAACGTCGAGAGATATCCTTGCAATTAAGACAGATTTCTCCGCCACAGCGTTTCCAAACGCCAAACAGACAATTCATCAGTTCAGTCCGTCCGGAACAGACAAGTCCGGcc          <  2:2024400/140‑2 (MQ=255)
                                                                                                                       cATCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCaa         >  1:1434756/1‑140 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                    >  1:1920292/1‑127 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                    <  2:1920292/127‑1 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg       >  2:1279955/1‑140 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg       <  1:1938174/140‑1 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg       >  2:2010465/1‑140 (MQ=255)
                                                                                                                         tCCCTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg       >  2:1474136/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          <  1:1698553/136‑1 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          >  1:587310/1‑136 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          <  2:587310/136‑1 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa          >  2:1698553/1‑136 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  1:26010/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  2:918640/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  1:1157198/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  1:1747366/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  1:2010275/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  1:904788/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  2:1333427/1‑140 (MQ=255)
                                                                                                                          cccTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc      >  2:1565368/1‑140 (MQ=255)
                                                                                                                            cTTTTTTCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCt    <  2:990474/140‑1 (MQ=255)
                                                                                                                              tttttCACGTCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa  >  1:791844/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                  
AACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAA  >  NC_000913/4309842‑4310107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A