Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,381,573 C→A R249L (CGC→CTC)  frdA ← fumarate reductase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,5730CA100.0% 424.6 / NA 123R249L (CGC→CTC) frdAfumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (64/59);  total (64/59)

Only 100 of 123 total aligned reads displayed.

CTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGG  >  NC_000913/4381440‑4381708
                                                                                                                                     |                                                                                                                                       
cTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAAc                                                                                                                                   <  1:763289/140‑1 (MQ=255)
   agagaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCtt                                                                                                                                >  2:1488449/1‑140 (MQ=255)
     agaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCg                                                                                                                              <  1:1071238/140‑1 (MQ=255)
     agaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCg                                                                                                                              <  1:1017821/140‑1 (MQ=255)
     agaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCg                                                                                                                              <  2:626669/140‑1 (MQ=255)
     agaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAATTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCg                                                                                                                              <  2:1615645/140‑1 (MQ=255)
        cTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGtc                                                                                                                           >  2:675655/1‑140 (MQ=255)
         tttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGtca                                                                                                                          >  1:1567474/1‑140 (MQ=255)
          ttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGtcat                                                                                                                         <  2:897702/140‑1 (MQ=255)
          ttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGtcat                                                                                                                         <  2:2013545/140‑1 (MQ=255)
           tGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGtcatc                                                                                                                        >  2:1764060/1‑140 (MQ=255)
                  tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCtt                                                                                                                                >  1:700007/1‑125 (MQ=255)
                  tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATAc                                                                                                                 <  2:2134510/140‑1 (MQ=255)
                  tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATAc                                                                                                                 <  2:1789131/140‑1 (MQ=255)
                  tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATAc                                                                                                                 <  2:1142365/140‑1 (MQ=255)
                  tGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATAc                                                                                                                 >  2:1757573/1‑140 (MQ=255)
                    gACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCg                                                                                                               <  1:1764060/140‑1 (MQ=255)
                           ttCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTg                                                                                                        >  1:85757/1‑140 (MQ=255)
                                tatatTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATTCCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGa                                                                                                   >  2:641495/1‑140 (MQ=255)
                                tatatTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGa                                                                                                   <  2:710786/140‑1 (MQ=255)
                                tatatTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGa                                                                                                   <  2:429747/140‑1 (MQ=255)
                                 atatTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGAc                                                                                                  <  2:246395/140‑1 (MQ=255)
                                   atTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCg                                                                                                >  1:1885127/1‑140 (MQ=255)
                                    tttGTTTTTCGGCTCGCCCAGCGGATTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg                                                                                               <  2:1779941/140‑1 (MQ=255)
                                    tttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg                                                                                               <  1:1757573/140‑1 (MQ=255)
                                    tttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg                                                                                               <  2:1567474/140‑1 (MQ=255)
                                    tttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg                                                                                               <  1:1674721/140‑1 (MQ=255)
                                    tttGTTTTTCGGCTCGCCCAGCGGAGTTGCCGGCCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGATGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg                                                                                               <  1:1659307/140‑1 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  2:709904/1‑140 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  2:1308230/1‑140 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  2:1217565/1‑140 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  1:1049781/1‑140 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGAAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  1:229807/1‑140 (MQ=255)
                                     ttGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGAAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                                                                              >  1:793400/1‑140 (MQ=255)
                                       gTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTg                                                                                            >  1:903577/1‑140 (MQ=255)
                                        tttttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTgg                                                                                           >  1:211956/1‑140 (MQ=255)
                                         ttttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTggg                                                                                          <  1:1217565/140‑1 (MQ=255)
                                         ttttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTggg                                                                                          >  1:255870/1‑140 (MQ=255)
                                          tttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGt                                                                                         >  2:69939/1‑140 (MQ=255)
                                          tttCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGt                                                                                         >  1:2058624/1‑140 (MQ=255)
                                                ctggCCCAGCGGATTTACCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACt                                                                                   <  2:1366603/137‑1 (MQ=255)
                                                   gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGaa                                                                                <  2:211956/140‑1 (MQ=255)
                                                    cccAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAAc                                                                               <  1:69939/140‑1 (MQ=255)
                                                         cGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAtt                                                                          <  2:484120/140‑1 (MQ=255)
                                                              tttCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCAtt                                                                     >  2:1690025/1‑139 (MQ=255)
                                                              tttCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                     >  2:1196424/1‑140 (MQ=255)
                                                              tttCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg                                                                     >  1:297613/1‑140 (MQ=255)
                                                                 ccGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCa                                                                  <  1:1488449/140‑1 (MQ=255)
                                                                 ccGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCa                                                                  <  2:254921/140‑1 (MQ=255)
                                                                   gggCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACg                                                                <  1:323331/140‑1 (MQ=255)
                                                                   gggCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACg                                                                >  2:2125630/1‑140 (MQ=255)
                                                                         aTGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCgg                                                          <  1:709904/140‑1 (MQ=255)
                                                                          tGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                         >  2:857186/1‑140 (MQ=255)
                                                                          tGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                         >  2:1591590/1‑140 (MQ=255)
                                                                          tGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                         >  1:2006583/1‑140 (MQ=255)
                                                                          tGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGa                                                         >  2:1238864/1‑140 (MQ=255)
                                                                           gCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGaa                                                        >  1:1160526/1‑140 (MQ=255)
                                                                           gCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCGTCACCGAGGCAACCTTCGGTCATCAGGATAGCGGAACCTGGCAGACCGGTTTGGTGATACTGAACGAATTCCATGTCACGCAGCGGaa                                                        >  1:2023056/1‑140 (MQ=255)
                                                                               tAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGcc                                                    >  1:1903013/1‑140 (MQ=255)
                                                                                aaTCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCg                                                   <  2:85757/140‑1 (MQ=255)
                                                                                aaTATTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGATTTCCATGTCACGCAGCGGAACGCCg                                                   <  2:1682538/140‑1 (MQ=255)
                                                                                     tGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCt                                              >  2:446182/1‑140 (MQ=255)
                                                                                     tGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCt                                              <  2:255870/140‑1 (MQ=255)
                                                                                      gCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCATCCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCtt                                             <  1:1196424/140‑1 (MQ=255)
                                                                                      gCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCtt                                             <  2:1363541/140‑1 (MQ=255)
                                                                                      gCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCtt                                             <  1:1690025/140‑1 (MQ=255)
                                                                                       cAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTa                                            <  2:1827358/140‑1 (MQ=255)
                                                                                       cAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTa                                            >  1:556885/1‑140 (MQ=255)
                                                                                        aGATAACGGTAGACATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGGCCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAg                                           <  1:2125630/140‑1 (MQ=255)
                                                                                           tAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAgcgc                                        <  1:167482/140‑1 (MQ=255)
                                                                                           aaaCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAgcgc                                        <  2:297613/139‑1 (MQ=255)
                                                                                            aaCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcc                                       <  1:446182/140‑1 (MQ=255)
                                                                                            aaCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcc                                       >  2:1003640/1‑140 (MQ=255)
                                                                                             aCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcca                                      <  2:1049781/140‑1 (MQ=255)
                                                                                             aCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcca                                      <  1:1457570/140‑1 (MQ=255)
                                                                                             aCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGcca                                      <  2:345550/140‑1 (MQ=255)
                                                                                                   gCCATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccataccca                                >  2:79153/1‑140 (MQ=255)
                                                                                                    ccATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccat                               >  1:572469/1‑140 (MQ=255)
                                                                                                    ccATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccat                               >  1:75052/1‑140 (MQ=255)
                                                                                                    ccATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccat                               >  1:324446/1‑140 (MQ=255)
                                                                                                     cATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccata                              <  1:1591590/140‑1 (MQ=255)
                                                                                                     cATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccata                              <  1:1238864/140‑1 (MQ=255)
                                                                                                     cATTTTTGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGccatacccata                              <  1:857186/140‑1 (MQ=255)
                                                                                                       tttttGTTGACCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCcatacccatacc                            <  2:1903013/140‑1 (MQ=255)
                                                                                                                aCCAGAATACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGt                   >  2:88351/1‑140 (MQ=255)
                                                                                                                     aaTACCGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGa              >  2:600562/1‑140 (MQ=255)
                                                                                                                          cGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATgccg         >  1:759122/1‑140 (MQ=255)
                                                                                                                          cGCCTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATgccg         >  2:1428630/1‑140 (MQ=255)
                                                                                                                            ccTTCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATgccgcc       >  1:1034512/1‑140 (MQ=255)
                                                                                                                              ttCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGt     >  1:1489176/1‑140 (MQ=255)
                                                                                                                              ttCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGt     <  2:75052/140‑1 (MQ=255)
                                                                                                                              ttCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGt     <  1:641495/140‑1 (MQ=255)
                                                                                                                               tCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGtt    >  1:142734/1‑140 (MQ=255)
                                                                                                                               tCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGtt    >  2:23212/1‑140 (MQ=255)
                                                                                                                               tCACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGtt    >  1:675458/1‑140 (MQ=255)
                                                                                                                                cACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGt     <  2:157130/138‑1 (MQ=255)
                                                                                                                                cACCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTg   <  1:1771567/140‑1 (MQ=255)
                                                                                                                                 aCCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTgg  >  1:1133504/1‑140 (MQ=255)
                                                                                                                                 aCCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTgg  <  1:233063/140‑1 (MQ=255)
                                                                                                                                 aCCGAGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTgg  >  1:502354/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                       
CTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGG  >  NC_000913/4381440‑4381708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A