Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F48 I1 R1	45	83.3	2696702	99.2%	2675128	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,694	C→A	A292S (GCC→TCC)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,694	0	C	A	100.0%	113.4 / NA	33	A292S (GCC→TCC)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/21); total (12/21)

CGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATT  >  NC_000913/2941557‑2941827

cgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGAtt                                                                                                                                     >  2:396739/1‑140 (MQ=255)

      aaaaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCa                                                                                                                               <  2:590373/140‑1 (MQ=255)

             cccAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCa                                                                                                                        <  2:1007192/140‑1 (MQ=255)

                          aaaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTA‑CCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAggcgg                                                                                                          <  1:660471/140‑1 (MQ=255)

                           aaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAggcgg                                                                                                          <  2:66143/140‑1 (MQ=255)

                            aTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCATGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAggcggc                                                                                                         >  2:1219244/1‑140 (MQ=255)

                            aTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGGCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAggcggc                                                                                                         >  2:910761/1‑140 (MQ=255)

                                aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                                                                                     <  1:134231/140‑1 (MQ=255)

                                aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                                                                                     <  1:396739/140‑1 (MQ=255)

                                             ggcgAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGGTATTTTACCCAGTTCTg                                                                                        <  2:102741/140‑1 (MQ=255)

                                                      cAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGt                                                                               <  1:1001018/140‑1 (MQ=255)

                                                          aTAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATg                                                                           <  2:978415/140‑1 (MQ=255)

                                                                cGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAAc                                                                     <  2:1292534/140‑1 (MQ=255)

                                                                cGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAAc                                                                     <  2:1256886/140‑1 (MQ=255)

                                                                cGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCATGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAAc                                                                     <  1:1219244/140‑1 (MQ=255)

                                                                 ggCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAAcc                                                                    <  2:209530/140‑1 (MQ=255)

                                                                   cTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAg                                                                  >  2:974572/1‑140 (MQ=255)

                                                                      gTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATa                                                               >  1:1286085/1‑140 (MQ=255)

                                                                      gTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATa                                                               >  2:1280520/1‑140 (MQ=255)

                                                                              cGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAt                                                       <  2:527426/140‑1 (MQ=255)

                                                                                tACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAttt                                                     >  1:1414/1‑140 (MQ=255)

                                                                                 aCCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAtttt                                                    >  1:1023983/1‑140 (MQ=255)

                                                                                   ccTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTa                                                  <  1:1084550/140‑1 (MQ=255)

                                                                                     tACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACt                                                <  1:681856/140‑1 (MQ=255)

                                                                                             ttattGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGTTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGaa                                        <  1:715014/140‑1 (MQ=255)

                                                                                                 tGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTACTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAAcatc                                    >  1:344303/1‑140 (MQ=255)

                                                                                                     cATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGACGAAAGGCGGCTATTTTACCCAGTCCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTa                                >  1:694960/1‑140 (MQ=255)

                                                                                                        aaCGAAAGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAAc                             >  2:98464/1‑140 (MQ=255)

                                                                                                              aGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGa                                         >  1:825445/1‑122 (MQ=255)

                                                                                                              aGCGGAATTTTTCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGa                                         <  2:825445/122‑1 (MQ=255)

                                                                                                                       tttCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGt              <  1:974572/140‑1 (MQ=255)

                                                                                                                       tttCTTGTTCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGt              <  1:1105131/140‑1 (MQ=255)

                                                                                                                       tttCTTGTTCAGCAGCGGATTTCGCCAGGAGTCAATGGCGAAAAGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGt              <  2:694960/140‑1 (MQ=255)

                                                                                                                              ttCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCt       <  1:1263775/140‑1 (MQ=255)

                                                                                                                              ttCAGCAGCGGATTTCGCCAGGATCCATTGGCGAAAGGCGGATATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAGCAAGACGTCCGGCCt       <  2:344303/140‑1 (MQ=255)

                                                                                                                                   cagcGGATTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGAtt  >  2:632886/1‑140 (MQ=255)

CGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATT  >  NC_000913/2941557‑2941827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A