Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F48 I1 R1 45 83.3 2696702 99.2% 2675128 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,058,923 C→A V135F (GTT→TTT)  rpiA ← ribose‑5‑phosphate isomerase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,058,9230CA100.0% 210.6 / NA 59V135F (GTT→TTT) rpiAribose‑5‑phosphate isomerase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/33);  total (26/33)

TCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCTTTGATCATTT  >  NC_000913/3058788‑3059054
                                                                                                                                       |                                                                                                                                   
tCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGTATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCt                                                                                                                                 >  2:1056786/1‑140 (MQ=255)
tCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCt                                                                                                                                 >  2:805348/1‑140 (MQ=255)
    gTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTg                                                                                                                             <  1:1056786/140‑1 (MQ=255)
          gATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCg                                                                                                                       <  1:805348/140‑1 (MQ=255)
              tCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGaa                                                                                                                   >  2:862080/1‑140 (MQ=255)
                   gCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAc                                                                                                              >  2:216171/1‑140 (MQ=255)
                    ccGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAcc                                                                                                             <  1:858183/140‑1 (MQ=255)
                    ccGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAcc                                                                                                             <  1:497196/140‑1 (MQ=255)
                    ccGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAcc                                                                                                             <  2:1131564/140‑1 (MQ=255)
                    ccGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAcc                                                                                                             <  1:393711/140‑1 (MQ=255)
                     cGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTAccc                                                                                                            <  2:353952/140‑1 (MQ=255)
                      gTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCa                                                                                                           <  1:795858/140‑1 (MQ=255)
                      gTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCa                                                                                                           <  2:183505/140‑1 (MQ=255)
                       tGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAg                                                                                                          >  1:981419/1‑140 (MQ=255)
                         gACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGaa                                                                                                        >  2:846599/1‑140 (MQ=255)
                              cGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCa                                                                                                   >  2:482174/1‑140 (MQ=255)
                               gAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCaa                                                                                                  >  2:426833/1‑140 (MQ=255)
                                aGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAAc                                                                                                 <  1:426833/140‑1 (MQ=255)
                                aGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAAc                                                                                                 <  1:748199/140‑1 (MQ=255)
                                   aTCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTg                                                                                              >  2:975816/1‑140 (MQ=255)
                                         ttGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCCCTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGa                                                                                        <  1:216171/140‑1 (MQ=255)
                                         ttGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGa                                                                                        <  2:1300054/140‑1 (MQ=255)
                                          tGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGaa                                                                                       <  2:998986/140‑1 (MQ=255)
                                           gCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAg                                                                                      >  1:638623/1‑140 (MQ=255)
                                            ccATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGc                                                                                     >  2:1046579/1‑140 (MQ=255)
                                            ccATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGc                                                                                     <  1:232274/140‑1 (MQ=255)
                                            ccATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCCCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATCCTACTAGCAGCGGGAACTTACCCAGAATATCAACCGGCTAGTAAGc                                                                                     >  1:1268490/1‑140 (MQ=255)
                                              aTTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGt                                                                                   <  1:1217355/140‑1 (MQ=255)
                                                    ggTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCaa                                                                             <  1:1046579/140‑1 (MQ=255)
                                                    ggTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCaa                                                                             >  2:192218/1‑140 (MQ=255)
                                                     gTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAAt                                                                            <  2:981419/140‑1 (MQ=255)
                                                          cacGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGa                                                                       >  1:858398/1‑140 (MQ=255)
                                                                  gACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAAt                                                                            <  1:576476/127‑1 (MQ=255)
                                                                  gACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAAt                                                                            <  1:1210928/127‑1 (MQ=255)
                                                                  gACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAAt                                                                            >  2:1210928/1‑127 (MQ=255)
                                                                  gACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAAt                                                                            >  2:576476/1‑127 (MQ=255)
                                                                    cGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTc                                                             >  1:822250/1‑140 (MQ=255)
                                                                     ggTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCt                                                            <  1:846599/140‑1 (MQ=255)
                                                                                   ccGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGTCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAAt                                              >  1:1317438/1‑140 (MQ=255)
                                                                                   ccGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAAt                                              >  1:31404/1‑140 (MQ=255)
                                                                                   ccGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAAt                                              <  1:1222682/140‑1 (MQ=255)
                                                                                   acGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAAt                                              <  2:1269414/139‑1 (MQ=255)
                                                                                      cccAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGAt                                           <  1:482174/140‑1 (MQ=255)
                                                                                      cccAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGAt                                           >  1:46511/1‑140 (MQ=255)
                                                                                             tCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAGCCGAAGCAATGATTTTTTCa                                    <  2:33064/140‑1 (MQ=255)
                                                                                             tCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCa                                    <  1:192218/140‑1 (MQ=255)
                                                                                                          cgcCACTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAgcgccg                       <  2:31404/140‑1 (MQ=255)
                                                                                                               cTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGcgccgcgccg                  <  2:638623/140‑1 (MQ=255)
                                                                                                               cTGCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGcgccgcgccg                  <  2:661131/140‑1 (MQ=255)
                                                                                                                 gCACTACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGcgccgcc                <  2:46511/140‑1 (MQ=255)
                                                                                                                     tACGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCt            >  1:748264/1‑140 (MQ=255)
                                                                                                                      aCGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCtt           <  2:1317438/140‑1 (MQ=255)
                                                                                                                       cGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCttt          >  2:736319/1‑140 (MQ=255)
                                                                                                                       cGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCttt          >  2:854741/1‑140 (MQ=255)
                                                                                                                       cGTGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCttt          >  2:958845/1‑140 (MQ=255)
                                                                                                                         tGCCATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCTTTGa        <  1:453778/140‑1 (MQ=255)
                                                                                                                            cATCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCTGCCTTTGATct     >  2:33911/1‑139 (MQ=255)
                                                                                                                             aTCGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCTTTGATCAt    <  2:858398/140‑1 (MQ=255)
                                                                                                                               cGGGATAAATTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCTTTGATCAttt  >  1:1135548/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAGATAAATTTTTCTGCAACCGAAGCAATGATTTTTTCACGGGTCAGCGCCGCGCCGCCGCCTTTGATCATTT  >  NC_000913/3058788‑3059054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A