Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F48 I1 R1	45	83.3	2696702	99.2%	2675128	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,352,017	C→A	D337Y (GAT→TAT)	arcB ←	sensory histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,352,017	0	C	A	100.0%	186.7 / NA	52	D337Y (GAT→TAT)	arcB	sensory histidine kinase ArcB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/26); total (26/26)

GTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTG  >  NC_000913/3351883‑3352152

gTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTaa                                                                                                                                    <  2:1318190/140‑1 (MQ=255)

gTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTaa                                                                                                                                    <  2:1153639/140‑1 (MQ=255)

gTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTaa                                                                                                                                    <  1:41907/140‑1 (MQ=255)

    aaGCGCAATCCTTTTTGTTGTGCCTGCAATGAGGCGGGATTTTACAGATCGGCAAGGAAGCTGGTGAAACAAACCGGATGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCACGTCGATAATATAGTTAAAGat                                                                                                                                >  2:300714/1‑140 (MQ=255)

    aaGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGat                                                                                                                                >  2:408683/1‑140 (MQ=255)

    aaGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGat                                                                                                                                >  2:698285/1‑140 (MQ=255)

         cAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTcc                                                                                                                           >  1:1057103/1‑140 (MQ=255)

          aaTCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGCTAATATAGTTAAAGATATTccc                                                                                                                          >  2:31963/1‑140 (MQ=255)

          aaTCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTccc                                                                                                                          >  2:498985/1‑140 (MQ=255)

            tCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCa                                                                                                                        <  1:726955/140‑1 (MQ=255)

                  ttgttgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGa                                                                                                                  <  1:499851/140‑1 (MQ=255)

                      tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGc                                                                                                              <  2:1340525/140‑1 (MQ=255)

                      tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGc                                                                                                              <  1:538693/140‑1 (MQ=255)

                      tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGc                                                                                                              <  2:1028530/140‑1 (MQ=255)

                      tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGc                                                                                                              <  2:211106/140‑1 (MQ=255)

                        cgcCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCg                                                                                                            >  1:558153/1‑140 (MQ=255)

                        cgcCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCg                                                                                                            >  1:583436/1‑140 (MQ=255)

                          ccTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGaa                                                                                                          <  2:558153/140‑1 (MQ=255)

                             gCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACa                                                                                                       >  2:1180387/1‑140 (MQ=255)

                                aTGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgg                                                                                                    <  2:1057103/140‑1 (MQ=255)

                                 tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                                                   <  1:408683/140‑1 (MQ=255)

                                 tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                                                   <  2:1139470/140‑1 (MQ=255)

                                  gCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatggat                                                                                                  <  1:1306756/140‑1 (MQ=255)

                                        agaTTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCtt                                                                                            >  1:248964/1‑140 (MQ=255)

                                        agaTTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCtt                                                                                            >  1:1214696/1‑140 (MQ=255)

                                                  gATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATAtttt                                                                                  >  2:719127/1‑140 (MQ=255)

                                                             aaGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTcggc                                                                       <  1:353711/140‑1 (MQ=255)

                                                                 tGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTg                                                                   >  2:390373/1‑140 (MQ=255)

                                                                  ggTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGa                                                                  <  2:248964/140‑1 (MQ=255)

                                                                   gTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAg                                                                 <  1:1180387/140‑1 (MQ=255)

                                                                     gAAATCAACCGGTTGATTATCAAGCTGGCCCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGtt                                                               <  1:31963/140‑1 (MQ=255)

                                                                             ccGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATcc                                                       >  1:756700/1‑140 (MQ=255)

                                                                               ggTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCcag                                                     <  1:894604/140‑1 (MQ=255)

                                                                               ggTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCcag                                                     >  1:839552/1‑140 (MQ=255)

                                                                                 ttGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCcagca                                                   <  1:719127/140‑1 (MQ=255)

                                                                                      tATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAAtt                                              >  2:77618/1‑139 (MQ=255)

                                                                                           aGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCt                                         >  2:1177457/1‑140 (MQ=255)

                                                                                              tGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCt                                         <  2:1231146/137‑1 (MQ=255)

                                                                                              tGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCt                                         >  1:1231146/1‑137 (MQ=255)

                                                                                                gACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGAc                                    <  1:698285/140‑1 (MQ=255)

                                                                                                   cTTGCGCCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGCCGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACcga                                 <  2:1214696/140‑1 (MQ=255)

                                                                                                        gcCGTTCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATa                            <  1:390373/140‑1 (MQ=255)

                                                                                                            ttCCATCTTATCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGt                        >  2:997375/1‑140 (MQ=255)

                                                                                                                      tCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCTGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGgtgt              >  2:537656/1‑140 (MQ=255)

                                                                                                                      tCCATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGgtgt              >  2:1233468/1‑140 (MQ=255)

                                                                                                                       ccATGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTa             >  2:675421/1‑140 (MQ=255)

                                                                                                                         aTGTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACg           >  2:151057/1‑140 (MQ=255)

                                                                                                                           gTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCa         >  1:1347899/1‑140 (MQ=255)

                                                                                                                           gTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCa         >  1:1284005/1‑140 (MQ=255)

                                                                                                                           gTCGATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCa         >  1:1073397/1‑140 (MQ=255)

                                                                                                                              gATAATATAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAAtt      >  2:311519/1‑140 (MQ=255)

                                                                                                                               ataataTAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTc     <  2:839552/140‑1 (MQ=255)

                                                                                                                                 aataTAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGt   >  2:233095/1‑140 (MQ=255)

                                                                                                                                  atatAGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTg  <  1:77265/140‑1 (MQ=255)

GTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTG  >  NC_000913/3351883‑3352152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A