Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F48 I1 R1 45 83.3 2696702 99.2% 2675128 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,480,378 G→A P77L (CCC→CTC)  kefB ← K(+) : H(+) antiporter KefB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,3780GA100.0% 196.1 / NA 55P77L (CCC→CTC) kefBK(+) : H(+) antiporter KefB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

GGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAA  >  NC_000913/3480241‑3480503
                                                                                                                                         |                                                                                                                             
ggCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGgagaga                                                                                                                             >  1:1158535/1‑140 (MQ=255)
  ccAATGCCCCCCCCCCCCGCCGCCTGCCAGGCAAATTCCGTCGGCTTCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGAtt                                                                                                                           <  1:1038950/140‑1 (MQ=255)
  caaacgCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGAtt                                                                                                                           <  2:672997/135‑1 (MQ=255)
  acAATCCCACCGACCCCCGCCGCCTGCCAGGCGAAACCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGAtt                                                                                                                           <  2:223166/139‑1 (MQ=255)
  aaaaTGCCACCGCCCACCGCCGCCGGCCAGGCGAAATCCGTCAGCATCAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGAtt                                                                                                                           <  2:1121109/138‑1 (MQ=255)
  aaaaTCCCACCCACCCCCCCCGCCTGCCAGGCGAAACCAGTCAGAAAAAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGAtt                                                                                                                           <  2:184845/138‑1 (MQ=255)
     aTGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCaa                                                                                                                        >  2:571175/1‑140 (MQ=255)
      tGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAAc                                                                                                                       >  1:413780/1‑140 (MQ=255)
          aCCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCaa                                                                                                                   >  1:195348/1‑140 (MQ=255)
           ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAg                                                                                                                  >  1:393430/1‑140 (MQ=255)
           ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAg                                                                                                                  >  2:203320/1‑140 (MQ=255)
           ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAg                                                                                                                  >  1:308862/1‑140 (MQ=255)
           ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAg                                                                                                                  >  1:244033/1‑140 (MQ=255)
           ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAg                                                                                                                  >  1:1183719/1‑140 (MQ=255)
             acccaccGCCGCCGGCCAGGCGAAATCCGTCAGCATAAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGc                                                                                                                <  2:413780/138‑1 (MQ=255)
              accaccGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGcc                                                                                                               >  1:556410/1‑140 (MQ=255)
                   cgccgcCCGCCAGGCGAAACCCGTAAGCAAAAATAACCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCgatga                                                                                                          <  2:556410/140‑1 (MQ=255)
                     ccgccTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATa                                                                                                        <  1:257323/140‑1 (MQ=255)
                     ccgccTGCCAGGCCAAAGCCCACCGCAAAAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATa                                                                                                        <  2:556521/140‑1 (MQ=255)
                      cgccTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATaa                                                                                                       <  1:203320/140‑1 (MQ=255)
                        ccTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAAc                                                                                                     >  1:1008605/1‑140 (MQ=255)
                        ccTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAAc                                                                                                     <  2:406266/140‑1 (MQ=255)
                                         gTCAGAATCAAAAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGcc                                                                                    <  1:394291/140‑1 (MQ=255)
                                          tCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCg                                                                                   >  2:980184/1‑140 (MQ=255)
                                            aGCATAAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAg                                                                                 <  2:46199/140‑1 (MQ=255)
                                              cATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGtt                                                                               >  2:871558/1‑140 (MQ=255)
                                              cATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGtt                                                                               <  2:195348/140‑1 (MQ=255)
                                                   ataatCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGaa                                                                          <  2:244033/140‑1 (MQ=255)
                                                   ataatCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGaa                                                                          <  2:1158535/140‑1 (MQ=255)
                                                   ataatCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGaa                                                                          >  2:461274/1‑140 (MQ=255)
                                                       tCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGt                                                                      >  2:1262289/1‑140 (MQ=255)
                                                           gCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGaa                                                                          >  1:1075276/1‑132 (MQ=255)
                                                           gCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGaa                                                                          <  2:1075276/132‑1 (MQ=255)
                                                           gCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggag                                                                  >  1:205638/1‑140 (MQ=255)
                                                           gCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCAGAGTTCCGAAAAGTggag                                                                  <  2:891881/140‑1 (MQ=255)
                                                            ccAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                                 >  2:1229646/1‑140 (MQ=255)
                                                            ccAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                                 >  1:994354/1‑140 (MQ=255)
                                                            ccAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                                 >  2:1123243/1‑140 (MQ=255)
                                                            ccAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                                 <  1:461274/140‑1 (MQ=255)
                                                            ccAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                                 >  1:453395/1‑140 (MQ=255)
                                                                       gcgcTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGAc                                                      <  1:1123243/140‑1 (MQ=255)
                                                                        cgcTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACg                                                     >  2:859888/1‑140 (MQ=255)
                                                                         gcTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCATGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                    <  2:1008605/140‑1 (MQ=255)
                                                                         gcTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                    <  1:980184/140‑1 (MQ=255)
                                                                          cTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTc                                                   >  1:24848/1‑140 (MQ=255)
                                                                                    ccTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAAc                                         >  2:128771/1‑140 (MQ=255)
                                                                                      tgtgCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAAccc                                       <  2:112753/140‑1 (MQ=255)
                                                                                       gtgCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAAcccc                                      >  2:832093/1‑140 (MQ=255)
                                                                                                     ccAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGcc                        <  1:571175/140‑1 (MQ=255)
                                                                                                              gAACGCCGCAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAAt               >  2:747622/1‑140 (MQ=255)
                                                                                                                      cAGTTGCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCa       <  1:859888/140‑1 (MQ=255)
                                                                                                                      aaGTTGCCAAAGTTTGGAGAGATGCAACTCAAGGCCGATGATATGCATCAGGAAGACCACGCCGAGTACCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCa       <  2:423239/139‑1 (MQ=255)
                                                                                                                           gCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAATCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  2:308862/140‑1 (MQ=255)
                                                                                                                           gCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  2:393430/140‑1 (MQ=255)
                                                                                                                           gCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  1:1229646/140‑1 (MQ=255)
                                                                                                                           gCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  1:1121354/140‑1 (MQ=255)
                                                                                                                           gCCAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  2:994354/140‑1 (MQ=255)
                                                                                                                           accAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCAAATAAACCCCAACCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa  <  2:205638/139‑1 (MQ=255)
                                                                                                                           aacAAAGTTTGGAGAGATTCAACTCAAGGCCGATGATAAACATCAGGAAAAACACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATAGCAATCCCTGCCAGCaaa  <  2:453395/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                             
GGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAATCCCGCCAGCAACGCCGCGCTTAACAGCACCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAA  >  NC_000913/3480241‑3480503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A