Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,227	C→A	P4T (CCG→ACG)	yheU →	UPF0270 family protein YheU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,227	0	C	A	99.1%	371.4 / ‑6.0	106	P4T (CCG→ACG)	yheU	UPF0270 family protein YheU
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (55/50); minor base T (1/0); total (56/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTG  >  NC_000913/3484094‑3484360

tGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGc                                                                                                                                  <  2:671785/140‑1 (MQ=255)

     tATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGAc                                                                                                                             <  2:311380/140‑1 (MQ=255)

       tCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACct                                                                                                                           <  1:611772/140‑1 (MQ=255)

       tCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACct                                                                                                                           <  1:789160/140‑1 (MQ=255)

       tCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACct                                                                                                                           <  2:1443808/140‑1 (MQ=255)

       acagctgactgaACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACct                                                                                                                           <  1:357375/136‑1 (MQ=255)

         aaCTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACctc‑t                                                                                                                        <  1:562597/140‑1 (MQ=255)

             gactgaACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTTATCCCCAAAAGTGGGTGGGGTGACGCCTTTCTTAC‑TGGGTTAAAAAGTATTTTGGGGGGGAAAAATGTTTGTTTCGTGGGCAGAACCC‑Tcccc                                                                                                                    >  2:1131860/1‑140 (MQ=255)

             gactgaACATGGCGGTCATGTTGGCTTTATTGGCGGGACAATACTTTATCCCCCAAAGTGGCTGGGGGCACCCATTTCTTAC‑TGGGTAACAAAGGATCTGGGGGGGGAAATATGTTTATTTCGTGGGAAAAACCC‑Tcccc                                                                                                                    >  2:1165571/1‑140 (MQ=255)

                  aaCATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGaaa                                                                                                               <  1:115052/140‑1 (MQ=255)

                  aaCATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCCCAAAAGTGGGTTGAGGTAAGCATTTCTTGC‑TTGGTTACAACGTATCTTGAGGCGGAAACATTTTGATTACCTGGGAAGACCTC‑TCCCCCCaaa                                                                                                               >  2:905608/1‑140 (MQ=255)

                      tGGCGGTCATGTTGGCTTTATTGGCGGTACAATACTTCATCCCCAAAAGTGGGTTGGGGCACGCCATCCTTACTTGGTTAA‑AACCTATCTTGGGGGGGAATCATTTTTTTTTCGTGGGAAAAACCC‑TCCCCCCaaaacgt                                                                                                           >  2:357375/1‑136 (MQ=255)

                             cATGTTGGCTTTATTGGCGGTAAATTAATTCATCCCCAAAAGTGGGTGGGGTCACGCATTCCTTTC‑TGGGTTAAAACGCATTTTGGGGGGAAAACATGTTGTTTACCTGGCGAGACCCCTTCCCCCGAAA‑GCTGGAAAAt                                                                                                    >  2:872691/1‑140 (MQ=255)

                                    gCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTg                                                                                             <  1:872292/140‑1 (MQ=255)

                                    gCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTg                                                                                             <  1:1165571/140‑1 (MQ=255)

                                     ttttATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACATCGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGa                                                                                            <  1:1368481/139‑1 (MQ=255)

                                     cTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGa                                                                                            <  1:122777/140‑1 (MQ=255)

                                       ttATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGaaa                                                                                          <  1:768602/140‑1 (MQ=255)

                                         aTTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGc                                                                                        <  2:1031370/140‑1 (MQ=255)

                                          ttGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCt                                                                                       <  2:1429484/140‑1 (MQ=255)

                                          ttGGCGGTACATTACTTCATCCGCAAATGTGGCTAGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCt                                                                                       <  1:905608/140‑1 (MQ=255)

                                           tGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCtt                                                                                      <  1:1114171/140‑1 (MQ=255)

                                                           cATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      >  1:1493335/1‑140 (MQ=255)

                                                           cATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      <  1:596353/140‑1 (MQ=255)

                                                           cATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      >  1:1088237/1‑140 (MQ=255)

                                                           cATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      >  1:1201668/1‑140 (MQ=255)

                                                           cATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      <  2:1238207/140‑1 (MQ=255)

                                                           cATCCGCAAATGTGGCTGAAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGc                                                                      >  1:427526/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:1497624/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  2:1363392/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:1714004/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:695254/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  2:962345/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:1114097/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:438113/1‑140 (MQ=255)

                                                             tCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    >  1:644510/1‑140 (MQ=255)

                                                             tCCGCAAATGCGGCTGGAATCACGCATTCCTGAC‑TGGTTACCAACGTATCTCGAGGCGCAATCATGTTCATCAACTGGCAAGACCTC‑TCCCCCGGAACGCTGGAAAATTTAATTAAAATCTTTGCTCTACGTTAAGGCAc                                                                    >  2:1431959/1‑140 (MQ=255)

                                                             accGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAc                                                                    <  2:598414/139‑1 (MQ=255)

                                                              ccGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAcc                                                                   >  2:759054/1‑140 (MQ=255)

                                                              ccGCAAATGTGGCTGGAGTCACGAATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCAcc                                                                   <  1:76943/140‑1 (MQ=255)

                                                               cGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCg                                                                  >  1:1221813/1‑140 (MQ=255)

                                                                gCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGa                                                                 <  2:695254/140‑1 (MQ=255)

                                                                   aaTGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTa                                                              <  2:978781/140‑1 (MQ=255)

                                                                     tgtgGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATg                                                            <  1:1131860/140‑1 (MQ=255)

                                                                     tgtgGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATg                                                            <  1:1638212/140‑1 (MQ=255)

                                                                       tgGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGt                                                          >  1:1046314/1‑140 (MQ=255)

                                                                        ggCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTg                                                         >  1:1316307/1‑140 (MQ=255)

                                                                         gCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGa                                                        >  2:254479/1‑140 (MQ=255)

                                                                         gCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGa                                                        >  2:1430646/1‑140 (MQ=255)

                                                                            ggAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACa                                                     >  1:777063/1‑140 (MQ=255)

                                                                             gAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACAt                                                    <  1:1363392/140‑1 (MQ=255)

                                                                             gAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACAt                                                    <  2:1497624/140‑1 (MQ=255)

                                                                             aaGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACAt                                                    <  2:427526/139‑1 (MQ=255)

                                                                               gTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGa                                                  >  2:1150470/1‑140 (MQ=255)

                                                                                    gCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTTCGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATTGTGAACATGAGCGTa                                             >  1:428097/1‑140 (MQ=255)

                                                                                       ttCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTacac                                          >  1:1689588/1‑140 (MQ=255)

                                                                                        tCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACt                                         >  1:172523/1‑140 (MQ=255)

                                                                                        tCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACt                                         >  2:614236/1‑140 (MQ=255)

                                                                                        tCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACt                                         <  2:924199/140‑1 (MQ=255)

                                                                                          cTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTg                                       <  1:850565/140‑1 (MQ=255)

                                                                                          atGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTg                                       <  2:1088237/139‑1 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  2:1169390/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:70004/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  2:1636282/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:149233/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:999594/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:1006830/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:1521175/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGaaa                               >  1:1704005/1‑140 (MQ=255)

                                                                                                   ttAACAACGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACACGAGCGTACACTTGAACAGaaa                               >  2:1028099/1‑140 (MQ=255)

                                                                                                     aacaacGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGt                             >  1:1121819/1‑140 (MQ=255)

                                                                                                     aacaacGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGt                             >  1:1494211/1‑140 (MQ=255)

                                                                                                         acGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGcc                         <  1:759054/140‑1 (MQ=255)

                                                                                                          cGTATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCg                        >  1:1530594/1‑140 (MQ=255)

                                                                                                            tATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                      >  2:993527/1‑140 (MQ=255)

                                                                                                            tATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                      >  1:1157502/1‑140 (MQ=255)

                                                                                                            tATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                      >  2:1059788/1‑140 (MQ=255)

                                                                                                            tATCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                      >  1:528287/1‑140 (MQ=255)

                                                                                                             aTCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGATCGTACACTTGAACAGAAAGTCGCCGAct                     >  2:1621135/1‑139 (MQ=255)

                                                                                                             aTCTGGAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAct                     >  2:152774/1‑139 (MQ=255)

                                                                                                                 ggAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACg                     <  1:1043045/136‑1 (MQ=255)

                                                                                                                 ggAGGCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACg                     >  2:1043045/1‑136 (MQ=255)

                                                                                                                    ggCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACg              >  1:1380475/1‑140 (MQ=255)

                                                                                                                    ggCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACg              >  1:79079/1‑140 (MQ=255)

                                                                                                                    ggCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACg              >  1:504986/1‑140 (MQ=255)

                                                                                                                    ggCGAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACg              >  1:588746/1‑140 (MQ=255)

                                                                                                                       gAAATCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGTTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGTTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCa           >  2:278653/1‑140 (MQ=255)

                                                                                                                        aaaTCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACTTGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAg          >  1:410621/1‑140 (MQ=255)

                                                                                                                        aaaTCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAg          <  1:962345/140‑1 (MQ=255)

                                                                                                                           tCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  1:683892/140‑1 (MQ=255)

                                                                                                                           tCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  2:1221813/140‑1 (MQ=255)

                                                                                                                           tCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  2:342001/140‑1 (MQ=255)

                                                                                                                           tCATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  2:1114097/140‑1 (MQ=255)

                                                                                                                           acATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  2:706208/139‑1 (MQ=255)

                                                                                                                           acATGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa       <  2:528287/139‑1 (MQ=255)

                                                                                                                             aTGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACa     <  2:1122152/140‑1 (MQ=255)

                                                                                                                              tGTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAg    >  1:1258092/1‑140 (MQ=255)

                                                                                                                               gTTGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGt   >  2:905011/1‑140 (MQ=255)

                                                                                                                                ttGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTg  <  1:614236/140‑1 (MQ=255)

                                                                                                                                ttGATTACGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTg  <  1:1073239/140‑1 (MQ=255)

TGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCATTCCTGAC‑TGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTC‑TCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTG  >  NC_000913/3484094‑3484360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A