Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,996,486	G→T	G67V (GGG→GTG)	xerC →	site‑specific tyrosine recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,996,486	0	G	T	100.0%	220.0 / NA	63	G67V (GGG→GTG)	xerC	site‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (39/24); total (39/24)

AGCCCGATAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCGA  >  NC_000913/3996350‑3996620

aGCCCGATAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCt                                                                                                                                     <  1:155196/140‑1 (MQ=255)

 gCCCGATAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTg                                                                                                                                    <  2:1784/140‑1 (MQ=255)

   ccGATAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTggg                                                                                                                                  <  1:973357/140‑1 (MQ=255)

      aTAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGagc                                                                                                                               >  2:1110904/1‑140 (MQ=255)

       tAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGagca                                                                                                                              >  1:849703/1‑140 (MQ=255)

        aaCCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGagcag                                                                                                                             <  2:491992/140‑1 (MQ=255)

        aaCCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGagcag                                                                                                                             <  2:1073305/140‑1 (MQ=255)

         aCCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGagcagc                                                                                                                            <  2:849703/140‑1 (MQ=255)

              gCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTc                                                                                                                       <  2:477758/140‑1 (MQ=255)

                ttAACTACCAGCGTCAGCTTGAGGAGATCATCAATTTTGCCAGCGAAAACGGACTGCAAAGCTAGCAGCAATGTGATGCGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAACTTGCTGGGAGCAGCAAGTCtt                                                                                                                     >  2:984595/1‑139 (MQ=255)

                       ccAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTAc                                                                                                              >  1:276454/1‑140 (MQ=255)

                         aGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACgt                                                                                                            >  2:330189/1‑139 (MQ=255)

                         aGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACgg                                                                                                            >  1:932571/1‑140 (MQ=255)

                           cGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCt                                                                                                          <  1:840516/140‑1 (MQ=255)

                            gTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCtt                                                                                                         >  2:339373/1‑140 (MQ=255)

                            gTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCtt                                                                                                         >  2:966360/1‑140 (MQ=255)

                             tCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCttt                                                                                                        <  1:1110904/140‑1 (MQ=255)

                               aGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCt                                                                                                      >  1:202668/1‑140 (MQ=255)

                                gCTTGAGGAGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTAGCAGCAATGTGATGAGACGATGGTGCGCAANTTTGCTGNACGCAGTCGCCGTAAAGTGCTGGNNNCAGCAAGTCTGGCGTTACGGCTTTCTg                                                                                                     <  1:984595/140‑1 (MQ=255)

                                 cTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTgc                                                                                                    <  1:828750/140‑1 (MQ=255)

                                     aGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTa                                                                                                >  2:785482/1‑140 (MQ=255)

                                     aGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTa                                                                                                >  1:984312/1‑140 (MQ=255)

                                         gATCATCAATTTTGCCAGCAAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTa                                                                                            <  2:1047160/140‑1 (MQ=255)

                                          atcatcAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAg                                                                                           >  2:961809/1‑140 (MQ=255)

                                          atcatcAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAg                                                                                           >  1:754585/1‑140 (MQ=255)

                                          atcatcAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAg                                                                                           >  1:8576/1‑140 (MQ=255)

                                           tcatcaATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGc                                                                                          >  1:83496/1‑140 (MQ=255)

                                           tcatcaATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGc                                                                                          >  1:225658/1‑140 (MQ=255)

                                                     tGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGAct                                                                                >  1:688363/1‑140 (MQ=255)

                                                     tGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGAct                                                                                >  1:336488/1‑140 (MQ=255)

                                                            gAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGActggctgat                                                                         <  1:789875/140‑3 (MQ=255)

                                                            gAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGt                                                                         <  2:276454/140‑1 (MQ=255)

                                                                    ccTGCAAAGCTGGCAGCAATGTGATGTGACGATGGGGAAAAACCTTGCTGGACTCAGGCGCAGTAAAGTGCTGGTAGCAGCATTTCTGGCGTTTCTGCTTTCTGCGCTACGTAGCTTTTGTGACTGGCTGGTCAgtccta                                                                 >  2:154445/1‑135 (MQ=255)

                                                                       gCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAgaacg                                                              >  2:146477/1‑140 (MQ=255)

                                                                        cAAAGCTGGCAGCAATGTGATGTGACGAGGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAgaacga                                                             >  2:844742/1‑140 (MQ=255)

                                                                          aaGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAgaacgaac                                                           <  2:83496/140‑1 (MQ=255)

                                                                            gCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTc                                                         <  1:1234658/140‑1 (MQ=255)

                                                                              tGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCaa                                                       >  2:1235148/1‑140 (MQ=255)

                                                                                       tgtgatgtgaCGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAAccc                                              >  1:1172478/1‑140 (MQ=255)

                                                                                             gtgaCGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGaa                                        <  2:946583/140‑1 (MQ=255)

                                                                                              tgaCGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGaaa                                       <  2:8576/140‑1 (MQ=255)

                                                                                              tgaCGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGaaa                                       >  2:226748/1‑140 (MQ=255)

                                                                                                 cGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGgt                                    >  2:1248065/1‑140 (MQ=255)

                                                                                                 cGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGgt                                    >  1:282279/1‑140 (MQ=255)

                                                                                                   aTGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCTGGCGAAAGgtgt                                  >  2:250798/1‑140 (MQ=255)

                                                                                                   aTGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGgtgt                                  >  2:1258025/1‑140 (MQ=255)

                                                                                                    tGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGtt                                 <  2:932571/140‑1 (MQ=255)

                                                                                                     ggTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGttt                                >  2:940175/1‑140 (MQ=255)

                                                                                                     ggTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGttt                                <  1:104746/140‑1 (MQ=255)

                                                                                                     ggTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGttt                                >  2:21594/1‑140 (MQ=255)

                                                                                                         cgcAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGc                            >  2:676049/1‑140 (MQ=255)

                                                                                                          gcAATTTTGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCa                           >  1:365833/1‑140 (MQ=255)

                                                                                                                 tGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAg                    <  2:688363/140‑1 (MQ=255)

                                                                                                                 tGCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAg                    <  2:832153/140‑1 (MQ=255)

                                                                                                                  gCTGTACGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAgc                   <  2:524761/140‑1 (MQ=255)

                                                                                                                        cGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCcgcg             >  2:827636/1‑140 (MQ=255)

                                                                                                                        cGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCcgcg             >  1:524535/1‑140 (MQ=255)

                                                                                                                        cGCAGTCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCcgcg             >  1:959653/1‑140 (MQ=255)

                                                                                                                             tCGCCGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATc        >  1:529053/1‑140 (MQ=255)

                                                                                                                                ccGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGc     >  2:181411/1‑140 (MQ=255)

                                                                                                                                ccGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGc     >  2:93498/1‑140 (MQ=255)

                                                                                                                                ccGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGc     >  1:327192/1‑140 (MQ=255)

                                                                                                                                ccGTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGc     >  2:968260/1‑140 (MQ=255)

                                                                                                                                  gTAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCg   <  2:272705/140‑1 (MQ=255)

                                                                                                                                   tAAAGTGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCGa  >  1:205480/1‑140 (MQ=255)

AGCCCGATAACCCTGCTTAACTACCAGCGTCAGCTTGAGGCGATCATCAATTTTGCCAGCGAAAACGGCCTGCAAAGCTGGCAGCAATGTGATGTGACGATGGTGCGCAATTTTGCTGTACGCAGTCGCCGTAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCGA  >  NC_000913/3996350‑3996620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A