Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,082,827	G→T	A999E (GCG→GAG)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,082,827	0	G	T	100.0%	192.2 / NA	54	A999E (GCG→GAG)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (27/27); total (27/27)

CGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTT  >  NC_000913/4082691‑4082957

cgcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCAt                                                                                                                                 >  1:1250464/1‑140 (MQ=255)

  ccTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCtcatca                                                                                                                               >  2:327596/1‑140 (MQ=255)

      aGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCaa                                                                                                                           >  2:1019776/1‑140 (MQ=255)

      aGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCaa                                                                                                                           >  2:447252/1‑140 (MQ=255)

          gcgGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGaa                                                                                                                       <  1:239560/140‑1 (MQ=255)

           cgGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATTTGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAAt                                                                                                                      >  1:14518/1‑140 (MQ=255)

           cgGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAAt                                                                                                                      >  1:683192/1‑140 (MQ=255)

            gggggTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATg                                                                                                                     >  1:90424/1‑140 (MQ=255)

            gggggTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATg                                                                                                                     <  2:93447/140‑1 (MQ=255)

             ggggTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATgg                                                                                                                    <  2:872822/140‑1 (MQ=255)

               ggTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCg                                                                                                                  <  1:1019776/140‑1 (MQ=255)

                  cAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTa                                                                                                               <  2:726865/140‑1 (MQ=255)

                    gACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTaaa                                                                                                             <  1:323327/140‑1 (MQ=255)

                    gACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTaaa                                                                                                             <  1:494644/140‑1 (MQ=255)

                        gTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTAt                                                                                                         >  1:1248541/1‑140 (MQ=255)

                        gTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTAt                                                                                                         >  2:675137/1‑140 (MQ=255)

                                cgGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATa                                                                                                 >  2:1005730/1‑140 (MQ=255)

                                   aaCGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAg                                                                                              <  1:404947/140‑1 (MQ=255)

                                    acgacgAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGc                                                                                             <  2:916286/140‑1 (MQ=255)

                                              atatCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGc                                                                                   <  1:127372/140‑1 (MQ=255)

                                                  cTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTa                                                                                 <  1:465726/138‑1 (MQ=255)

                                                  cTTGAGATTGATAAGCCATATGTCGTCTCCGGTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTa                                                                                 >  2:465726/1‑138 (MQ=255)

                                                    tGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGcc                                                                             <  2:146009/140‑1 (MQ=255)

                                                    tGAGATTGATAAGCCATATGTCGTCTCCGTTACAACTTTTCCACATTCACATGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGcc                                                                             >  2:617728/1‑140 (MQ=255)

                                                        aTTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCa                                                                         <  1:528350/140‑1 (MQ=255)

                                                        aTTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCa                                                                         <  1:447252/140‑1 (MQ=255)

                                                         ttGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGc                                                                                   <  2:1093299/129‑1 (MQ=255)

                                                         ttGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGc                                                                                   >  1:1093299/1‑129 (MQ=255)

                                                           gATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAg                                                                      >  2:30234/1‑140 (MQ=255)

                                                            aTAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGc                                                                     >  1:189915/1‑140 (MQ=255)

                                                                    tatGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGa                                                             >  2:256755/1‑140 (MQ=255)

                                                                      tGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAAt                                                           >  1:1099329/1‑140 (MQ=255)

                                                                      tGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAAt                                                           <  1:327596/140‑1 (MQ=255)

                                                                          gtcTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGa                                                       <  1:1020031/140‑1 (MQ=255)

                                                                           tctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGaa                                                      >  1:985234/1‑140 (MQ=255)

                                                                           tctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGaa                                                      >  1:881972/1‑140 (MQ=255)

                                                                           tctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGaa                                                      >  1:822377/1‑140 (MQ=255)

                                                                           tctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGaa                                                      >  1:53651/1‑140 (MQ=255)

                                                                           tctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGaa                                                      >  1:111983/1‑140 (MQ=255)

                                                                            ctcCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAAt                                                     <  1:256755/140‑1 (MQ=255)

                                                                              ccGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATAc                                                   <  2:985234/140‑1 (MQ=255)

                                                                                gTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCg                                                 <  2:348105/140‑1 (MQ=255)

                                                                                        ttttCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATc                                         <  2:1248541/140‑1 (MQ=255)

                                                                                          ttCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGa                                       <  1:74310/140‑1 (MQ=255)

                                                                                           tCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGat                                      >  2:539168/1‑140 (MQ=255)

                                                                                                 ttCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCttt                                >  2:242392/1‑140 (MQ=255)

                                                                                                    acaAGGAAGGACTTAAACTCAGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGcc                             >  1:928010/1‑140 (MQ=255)

                                                                                                     caAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCg                            <  1:2905/140‑1 (MQ=255)

                                                                                                       aggaaggaCTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGtt                          >  2:1089851/1‑140 (MQ=255)

                                                                                                            ggaCTTAAACTCCGGCGCCTGCGCGTTCTCATAACCAACGAATGGCGTTAAAGAATGGGCAATAAAGCCTTTTTTAGCTACGCCATCATAGCCCCAGCGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCtt                     >  2:58877/1‑140 (MQ=255)

                                                                                                             gaCTTAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCttt                    <  2:189915/140‑1 (MQ=255)

                                                                                                                 tAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGc                <  2:14518/140‑1 (MQ=255)

                                                                                                                 tAAACTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGc                <  1:675137/140‑1 (MQ=255)

                                                                                                                  aaaCTCCGGCGTCTGCGTGTTCTCAGCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGct               >  2:989383/1‑139 (MQ=255)

                                                                                                                   aaCTCCGGCGTCTGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGt              <  1:989383/140‑1 (MQ=255)

                                                                                                                               tGCGTGTTCTCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGttt  >  1:819296/1‑140 (MQ=255)

CGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTT  >  NC_000913/4082691‑4082957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A