Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,861	C→A	G321V (GGC→GTC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,861	0	C	A	100.0%	173.3 / NA	49	G321V (GGC→GTC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/26); total (23/26)

TTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCG  >  NC_000913/4084728‑4084992

tttCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCtt                                                                                                                               <  2:1410410/140‑1 (MQ=255)

  tCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCa                                                                                                                             <  2:12920/140‑1 (MQ=255)

  tCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCa                                                                                                                             <  1:516634/140‑1 (MQ=255)

   cAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCaa                                                                                                                            <  2:406847/140‑1 (MQ=255)

          ttCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTa                                                                                                                     <  2:1026528/140‑1 (MQ=255)

               cacGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCt                                                                                                                <  2:576354/140‑1 (MQ=255)

                    aGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTcacgc                                                                                                           >  1:796586/1‑140 (MQ=255)

                     gcgcGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTcacgca                                                                                                          <  1:1264481/140‑1 (MQ=255)

                      cgcgGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTcacgcac                                                                                                         >  1:1312407/1‑140 (MQ=255)

                               tGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCt                                                                                                <  2:1039648/140‑1 (MQ=255)

                                 cAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTgg                                                                                              <  2:461574/140‑1 (MQ=255)

                                   gCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCg                                                                                            <  1:123373/140‑1 (MQ=255)

                                    cGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTTCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGt                                                                                           <  1:693276/140‑1 (MQ=255)

                                    cGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGt                                                                                           <  2:1312407/140‑1 (MQ=255)

                                         tATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGta                                                                                      >  1:253581/1‑140 (MQ=255)

                                          aTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGtat                                                                                     <  2:789320/140‑1 (MQ=255)

                                          aTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGtat                                                                                     <  2:1313645/140‑1 (MQ=255)

                                                tttGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCtt                                                                               >  2:1472413/1‑140 (MQ=255)

                                                tttGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCtt                                                                               >  1:269551/1‑140 (MQ=255)

                                                  tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCtt                                                                               <  1:1198634/138‑1 (MQ=255)

                                                  tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCtt                                                                               >  2:1198634/1‑138 (MQ=255)

                                                  tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCg                                                                             >  2:640161/1‑140 (MQ=255)

                                                  tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCg                                                                             <  1:269224/140‑1 (MQ=255)

                                                  tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTC‑AAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCgg                                                                            >  1:232679/1‑140 (MQ=255)

                                                        aGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTAt                                                                       >  1:1481256/1‑140 (MQ=255)

                                                              tttCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCgg                                                                 >  2:1025663/1‑140 (MQ=255)

                                                                tCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGtt                                                               <  2:959895/140‑1 (MQ=255)

                                                                tCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGtt                                                               <  1:154909/140‑1 (MQ=255)

                                                                        ttCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAAttttt                                                       <  2:269551/140‑1 (MQ=255)

                                                                               gTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGtt                                                <  1:1025663/140‑1 (MQ=255)

                                                                                  cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcag                                             >  1:180427/1‑140 (MQ=255)

                                                                                   aggaggATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagc                                            <  2:1481256/140‑1 (MQ=255)

                                                                                    ggaggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagca                                           >  2:221453/1‑140 (MQ=255)

                                                                                     gaggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagcag                                          >  1:546113/1‑140 (MQ=255)

                                                                                     gaggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagcag                                          >  2:592301/1‑140 (MQ=255)

                                                                                      aggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAgg                                         <  1:1299163/140‑1 (MQ=255)

                                                                                          tttATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACa                                     >  2:1224568/1‑140 (MQ=255)

                                                                                             aTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCa                                  <  1:1472413/140‑1 (MQ=255)

                                                                                                  aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATAcc                             >  2:161644/1‑139 (MQ=255)

                                                                                                   cTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCATCAGGTACAGCAATACGc                            >  2:608418/1‑140 (MQ=255)

                                                                                                   cTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGc                            >  1:447407/1‑140 (MQ=255)

                                                                                                   cTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGc                            >  1:520593/1‑140 (MQ=255)

                                                                                                   cTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGc                            >  1:1552629/1‑140 (MQ=255)

                                                                                                    ttGCGTTTTTCCGCGTCGTAGCCGGTTAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGCTCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGcc                           >  2:1399436/1‑140 (MQ=255)

                                                                                                     tGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCt                          <  2:835673/140‑1 (MQ=255)

                                                                                                         tttttCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACa                      <  1:242239/140‑1 (MQ=255)

                                                                                                            ttCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGa                   >  1:818398/1‑140 (MQ=255)

                                                                                                            ttCCGCGTCGTAGCCGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGa                   >  1:570671/1‑140 (MQ=255)

                                                                                                                          cGGTGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTa     <  2:520593/140‑1 (MQ=255)

                                                                                                                             tGAACAGGACATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCg  >  1:1537878/1‑140 (MQ=255)

TTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCG  >  NC_000913/4084728‑4084992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A