Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,140	C→A	P1077P (CCG→CCT)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,140	0	C	A	100.0%	217.9 / NA	61	P1077P (CCG→CCT)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/34); total (27/34)

TCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGAT  >  NC_000913/4386003‑4386276

tCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAgg                                                                                                                                        >  2:729530/1‑140 (MQ=255)

 cAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAagggaggg                                                                                                                                       <  2:232047/140‑1 (MQ=255)

    ttttGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAAt                                                                                                                                    <  2:667837/140‑1 (MQ=255)

      ttgtttgtGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATgg                                                                                                                                  <  1:1222400/140‑1 (MQ=255)

        gtttgtGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCa                                                                                                                                <  1:1054576/140‑1 (MQ=255)

         tttgtGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCat                                                                                                                               >  1:54812/1‑140 (MQ=255)

          ttgtGAGTCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCata                                                                                                                              <  2:459441/140‑1 (MQ=255)

          ttgtGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCata                                                                                                                              >  1:213273/1‑140 (MQ=255)

          ttgtGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCata                                                                                                                              >  1:264645/1‑140 (MQ=255)

              gAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGTCCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGa                                                                                                                          <  1:1138142/140‑1 (MQ=255)

                   ggATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCg                                                                                                                     <  2:461163/140‑1 (MQ=255)

                   ggATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCg                                                                                                                     >  1:845572/1‑140 (MQ=255)

                          ttCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATGTCGATACCg                                                                                                                     >  2:1003818/1‑133 (MQ=255)

                          ttCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCg                                                                                                                     <  1:1003818/133‑1 (MQ=255)

                              tCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGaa                                                                                                          >  1:615743/1‑140 (MQ=255)

                               ccGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCTCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAag                                                                                                         <  1:801874/140‑1 (MQ=255)

                                cGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAagc                                                                                                        <  2:342696/140‑1 (MQ=255)

                                   cacacaAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAagccag                                                                                                     >  1:409755/1‑140 (MQ=255)

                                    acacaAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAagccagc                                                                                                    <  1:353345/140‑1 (MQ=255)

                                    acacaAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAagccagc                                                                                                    <  1:778888/140‑1 (MQ=255)

                                        aaacttacaaacttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAag                                                                                                         >  2:1214240/1‑131 (MQ=255)

                                        aaacttacaaacttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAag                                                                                                         <  1:1214240/131‑1 (MQ=255)

                                         aacttacaaacttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGaa                                                                                               >  1:15440/1‑140 (MQ=255)

                                            ttacaaacttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCa                                                                                            <  1:278094/140‑1 (MQ=255)

                                              acaaacttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGc                                                                                          <  2:54812/140‑1 (MQ=255)

                                                  acttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggt                                                                                      <  1:362055/140‑1 (MQ=255)

                                                  acttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggt                                                                                      <  1:1219837/140‑1 (MQ=255)

                                                   cttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggtg                                                                                     <  2:122224/140‑1 (MQ=255)

                                                    ttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggtgg                                                                                    >  1:684068/1‑140 (MQ=255)

                                                    ttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggtgg                                                                                    >  1:813685/1‑140 (MQ=255)

                                                    ttCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtggtgg                                                                                    >  2:764224/1‑140 (MQ=255)

                                                      cccGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAt                                                                                  >  2:91017/1‑140 (MQ=255)

                                                          ccTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCa                                                                              >  1:1211481/1‑140 (MQ=255)

                                                              acgacCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGc                                                                          >  1:103530/1‑140 (MQ=255)

                                                              acgacCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGc                                                                          >  1:429788/1‑140 (MQ=255)

                                                                  ccTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAg                                                                      <  2:248446/140‑1 (MQ=255)

                                                                      tGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGc                                                                  <  2:264645/140‑1 (MQ=255)

                                                                          cGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATAc                                                              <  2:915784/140‑1 (MQ=255)

                                                                           gCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACg                                                             <  2:615743/140‑1 (MQ=255)

                                                                            cAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACgg                                                            <  2:1211481/140‑1 (MQ=255)

                                                                                 gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGAcc                                                       <  1:711176/140‑1 (MQ=255)

                                                                                        ttCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCa                                                >  2:223749/1‑140 (MQ=255)

                                                                                            ccccGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAgcgg                                            <  1:764224/140‑1 (MQ=255)

                                                                                            ccccGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAgcgg                                            <  2:429788/140‑1 (MQ=255)

                                                                                            ccccGGTTGGTTAACGTAGATGATTTTGAGACGCACCTGGAAGAGAGGGAATGGCATAACGAGACCGTGGACATCGAAGCCAGCGCGAATCAGCTGGTGGATCTCATGGAGTAGCGGCATAAGGTGACCCATCTCAgcgg                                            <  2:435682/140‑1 (MQ=255)

                                                                                              ccGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAgcggcg                                          <  1:91017/140‑1 (MQ=255)

                                                                                                      ttACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACg                                  <  2:409755/140‑1 (MQ=255)

                                                                                                           gTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAgcgg                                            >  2:349375/1‑125 (MQ=255)

                                                                                                           gTTGAGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAgcgg                                            <  1:349375/125‑1 (MQ=255)

                                                                                                               aGGCTTTCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGaa                         <  2:813685/140‑1 (MQ=255)

                                                                                                                    ttCCAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCt                    >  1:837454/1‑140 (MQ=255)

                                                                                                                      ccAGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGa                  <  1:674920/140‑1 (MQ=255)

                                                                                                                       aaGACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGaa                 <  2:213273/139‑1 (MQ=255)

                                                                                                                         gACGCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATc               <  1:729530/140‑1 (MQ=255)

                                                                                                                            gCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATcccc            >  2:355269/1‑140 (MQ=255)

                                                                                                                            gCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATcccc            <  1:267612/140‑1 (MQ=255)

                                                                                                                            gCATCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATcccc            <  2:103530/140‑1 (MQ=255)

                                                                                                                               tCTGGTTGGGAGGGAATGGGATATCGATACCGTGGTCATGCAAGCCAGCCAGAATCAGCTGGAGAATCTCATGGCGTAGCAGCATACGGTGACCCATCTCATCGGCGTAAATACGCAGCTCGAAAATCTGAATCCTCTGt         >  2:1246097/1‑140 (MQ=255)

                                                                                                                               tCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGt         >  2:848506/1‑140 (MQ=255)

                                                                                                                               tCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGt         >  1:843973/1‑140 (MQ=255)

                                                                                                                               tCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGt         >  1:277860/1‑140 (MQ=255)

                                                                                                                               tCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGCGGATCTCATGGCGTAGCGTCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTAAATCCCCTGt         >  2:379546/1‑140 (MQ=255)

                                                                                                                               tCTGGAAGGGAGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCCGGTGGATCTCATGGCGTAGCGGCATACGGGGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGt         >  2:975930/1‑140 (MQ=255)

                                                                                                                                      gggagggaATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGAt  >  1:1002052/1‑140 (MQ=255)

TCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGAT  >  NC_000913/4386003‑4386276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A