Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F48 I1 R1	61	131.2	4447032	99.1%	4407008	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,402,523	G→T	S270R (AGC→AGA)	csrD ←	regulator of CsrB and CsrC decay

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,402,523	0	G	T	99.1%	386.2 / ‑3.8	108	S270R (AGC→AGA)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (47/60); total (48/60)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 115 total aligned reads displayed.

CGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGG  >  NC_000913/3402386‑3402659

cGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATctct                                                                                                                                        >  1:1972560/1‑140 (MQ=255)

cGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATctct                                                                                                                                        <  1:1666278/140‑1 (MQ=255)

cGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATctct                                                                                                                                        <  2:242967/140‑1 (MQ=255)

 ggTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTAtctctc                                                                                                                                       <  2:1990388/140‑1 (MQ=255)

  gTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATctct                                                                                                                                        >  1:628883/1‑138 (MQ=255)

  gTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCa                                                                                                                                      <  2:724423/140‑1 (MQ=255)

     cGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACa                                                                                                                                   >  1:723188/1‑140 (MQ=255)

       gTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACata                                                                                                                                 >  2:2131107/1‑140 (MQ=255)

       gTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACata                                                                                                                                 >  1:1609529/1‑140 (MQ=255)

        tAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACatat                                                                                                                                >  2:658882/1‑140 (MQ=255)

             gcgcAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGaaa                                                                                                                           >  1:1321668/1‑140 (MQ=255)

             gcgcAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGaaa                                                                                                                           <  1:3418/140‑1 (MQ=255)

               gcagcaAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATc                                                                                                                         <  2:723188/140‑1 (MQ=255)

                cagcaAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGATAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATcc                                                                                                                        >  2:191123/1‑140 (MQ=255)

                aagcaAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATcc                                                                                                                        <  2:320719/139‑1 (MQ=255)

                    aaaaTCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCa                                                                                                                    <  2:1870392/140‑1 (MQ=255)

                      aaTCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGa                                                                                                                  >  2:1623322/1‑140 (MQ=255)

                          aCTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGaa                                                                                                              <  1:1890347/140‑1 (MQ=255)

                           cTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAat                                                                                                             <  2:1201486/140‑1 (MQ=255)

                            tGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatc                                                                                                            >  1:1118807/1‑140 (MQ=255)

                            tGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatc                                                                                                            <  1:372372/140‑1 (MQ=255)

                             gCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatca                                                                                                           >  1:1276537/1‑140 (MQ=255)

                             gCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatca                                                                                                           >  1:1880652/1‑140 (MQ=255)

                              cGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatcat                                                                                                          <  2:800724/140‑1 (MQ=255)

                                gtggtAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAatcatca                                                                                                        >  1:799972/1‑140 (MQ=255)

                                   gtAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAat                                                                                                             >  1:9554/1‑132 (MQ=255)

                                    tAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCAc                                                                                                    <  1:1663155/140‑1 (MQ=255)

                                     aaCGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACg                                                                                                   <  2:1260192/140‑1 (MQ=255)

                                     aaCGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACg                                                                                                   <  1:658882/140‑1 (MQ=255)

                                     aaCGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACg                                                                                                   <  1:1239758/140‑1 (MQ=255)

                                     aaCGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACg                                                                                                   <  2:1118807/140‑1 (MQ=255)

                                       cgcgCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGGAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGAt                                                                                                 <  2:1609529/140‑1 (MQ=255)

                                       cgcgCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGAt                                                                                                 <  1:456692/140‑1 (MQ=255)

                                       agcgcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGAt                                                                                                 <  2:920437/139‑1 (MQ=255)

                                        gcgcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATc                                                                                                <  2:591010/140‑1 (MQ=255)

                                        gcgcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATc                                                                                                >  2:89075/1‑140 (MQ=255)

                                          gcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATccc                                                                                              >  2:492873/1‑140 (MQ=255)

                                          gcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATccc                                                                                              <  1:728876/140‑1 (MQ=255)

                                           ccAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCTCGATCCCg                                                                                             >  2:2070598/1‑140 (MQ=255)

                                           ccAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCg                                                                                             <  1:1305676/140‑1 (MQ=255)

                                           ccAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCg                                                                                             <  1:1679568/140‑1 (MQ=255)

                                               cagTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTggg                                                                                         <  2:264737/140‑1 (MQ=255)

                                                agTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGt                                                                                        <  1:2143612/140‑1 (MQ=255)

                                                  tGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTAc                                                                                      >  1:1168546/1‑140 (MQ=255)

                                                  tGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTAc                                                                                      <  1:230587/140‑1 (MQ=255)

                                                  tGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTAc                                                                                      <  1:1687780/140‑1 (MQ=255)

                                                       cAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACt                                                                                 <  2:1276537/140‑1 (MQ=255)

                                                       cAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACt                                                                                 <  2:1168546/140‑1 (MQ=255)

                                                       cAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACt                                                                                 <  1:157898/140‑1 (MQ=255)

                                                       cAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACt                                                                                 <  1:492873/140‑1 (MQ=255)

                                                       cAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACt                                                                                 <  1:2158239/140‑1 (MQ=255)

                                                          ggTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTtc                                                                              >  1:650105/1‑140 (MQ=255)

                                                          ggTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTtc                                                                              >  1:1647966/1‑140 (MQ=255)

                                                              gcgcATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCt                                                                          >  1:268885/1‑140 (MQ=255)

                                                              gcgcATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCt                                                                          <  2:650105/140‑1 (MQ=255)

                                                               cgcATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTg                                                                         >  1:2009381/1‑140 (MQ=255)

                                                                 catcatAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGAt                                                                       <  1:751959/140‑1 (MQ=255)

                                                                 catcatAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGAt                                                                       <  2:1880652/140‑1 (MQ=255)

                                                                  atcatAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATc                                                                      <  2:938115/140‑1 (MQ=255)

                                                                        aaTGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAg                                                                <  2:799972/140‑1 (MQ=255)

                                                                            tCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAAc                                                            >  2:2093729/1‑140 (MQ=255)

                                                                             cGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACg                                                           <  2:368837/140‑1 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  2:2168791/1‑140 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  2:307775/1‑140 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  1:811040/1‑140 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  2:592569/1‑140 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  2:623812/1‑140 (MQ=255)

                                                                              gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGt                                                          >  1:996588/1‑140 (MQ=255)

                                                                                 agcagcTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGc                                                       <  2:1363749/140‑1 (MQ=255)

                                                                                 agcagATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGc                                                       <  1:1623322/140‑1 (MQ=255)

                                                                                      aTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAg                                                                <  2:654538/126‑1 (MQ=255)

                                                                                      aTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAg                                                                >  1:654538/1‑126 (MQ=255)

                                                                                      aTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACt                                                  >  2:1261970/1‑140 (MQ=255)

                                                                                         cGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGAt                                               <  2:473078/140‑1 (MQ=255)

                                                                                               agTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGa                                         >  1:1431592/1‑140 (MQ=255)

                                                                                                 tGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGaaa                                       <  1:341203/140‑1 (MQ=255)

                                                                                                 tGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGaaa                                       >  1:2216679/1‑140 (MQ=255)

                                                                                                     gaaCTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAaga                                   <  2:268885/140‑1 (MQ=255)

                                                                                                     gaaCTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAaga                                   <  1:534171/140‑1 (MQ=255)

                                                                                                         tGTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCg                               >  1:1528579/1‑140 (MQ=255)

                                                                                                          gTTCTTCAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCgg                              <  2:2009381/140‑1 (MQ=255)

                                                                                                             cttcAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGttt                           >  2:1190156/1‑139 (MQ=255)

                                                                                                                cAACCTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTg                        >  1:769187/1‑140 (MQ=255)

                                                                                                                    cTGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGc                    <  2:1393433/140‑1 (MQ=255)

                                                                                                                     tGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGcc                   >  1:1543317/1‑140 (MQ=255)

                                                                                                                     tGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGcc                   >  1:500871/1‑140 (MQ=255)

                                                                                                                     tGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGcc                   <  1:1489627/140‑1 (MQ=255)

                                                                                                                     tGGCTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGcc                   >  1:1153180/1‑140 (MQ=255)

                                                                                                                     tGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCTTCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGcc                   >  1:423336/1‑140 (MQ=255)

                                                                                                                        cTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGt                >  1:2027792/1‑140 (MQ=255)

                                                                                                                        cTGTGCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGAACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGt                >  1:400255/1‑140 (MQ=255)

                                                                                                                            gCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTc            <  1:307775/140‑1 (MQ=255)

                                                                                                                            gCCCCCAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTc            <  1:191123/140‑1 (MQ=255)

                                                                                                                             cccccAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCa           >  2:496357/1‑140 (MQ=255)

                                                                                                                             cccccAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCa           >  2:549394/1‑140 (MQ=255)

                                                                                                                             cccccAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCa           >  2:1838105/1‑140 (MQ=255)

                                                                                                                                 cAGGTATCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTc       >  2:1566587/1‑140 (MQ=255)

                                                                                                                                      aTCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg  <  2:736272/140‑1 (MQ=255)

                                                                                                                                      aTCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg  <  2:811040/140‑1 (MQ=255)

                                                                                                                                      aTCTCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg  <  1:72541/140‑1 (MQ=255)

CGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGG  >  NC_000913/3402386‑3402659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A