Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,404,152	G→T	V223V (GTG→GTT)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,152	0	G	T	100.0%	172.0 / NA	49	V223V (GTG→GTT)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (28/21); total (28/21)

TCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGAT  >  NC_000913/3404020‑3404281

tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCaa                                                                                                                            >  2:61047/1‑140 (MQ=255)

    gAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGt                                                                                                                        <  1:1141929/140‑1 (MQ=255)

           cccATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGgcg                                                                                                                 <  2:1370942/140‑1 (MQ=255)

              aTGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCaa                                                                                                              >  2:861712/1‑140 (MQ=255)

               tGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCaaa                                                                                                             <  2:1261092/140‑1 (MQ=255)

                  atatCTGAAAAGTTTAGGTACTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGa                                                                                                          >  1:639115/1‑140 (MQ=255)

                   tatCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGaa                                                                                                         >  1:1195567/1‑140 (MQ=255)

                     tCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAAtt                                                                                                       >  2:181846/1‑140 (MQ=255)

                      cTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTa                                                                                                      >  1:914037/1‑140 (MQ=255)

                      cTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGCCTGGGCTGGGGCAATTGACACCGTTGCCGACAAAGTTCTGGAAAAAGTGCTGGCGTAAATGAtttc                                                                                                      >  2:161437/1‑139 (MQ=255)

                        gAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTAcg                                                                                                    >  2:976768/1‑140 (MQ=255)

                          aaaGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGGGCTGGCGCAAATGAATTAcggc                                                                                                  >  1:24271/1‑140 (MQ=255)

                              tttAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCt                                                                                              >  2:801458/1‑140 (MQ=255)

                              tttAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCt                                                                                              >  2:1056568/1‑140 (MQ=255)

                                tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGc                                                                                            >  1:1070493/1‑140 (MQ=255)

                                tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGc                                                                                            >  1:1314459/1‑140 (MQ=255)

                                   gTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTg                                                                                         <  1:391101/140‑1 (MQ=255)

                                   gTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTg                                                                                         <  2:1023182/140‑1 (MQ=255)

                                            gtgtTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCtgt                                                                                <  1:161437/140‑1 (MQ=255)

                                                 ctcCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCt                                                                           >  2:814802/1‑140 (MQ=255)

                                                       cGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCggg                                                                     <  2:1195567/140‑1 (MQ=255)

                                                       cGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCggg                                                                     <  2:733713/140‑1 (MQ=255)

                                                         tgatgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCCGCGTTGCGGCCCTTTGTCTGGCggcag                                                                   >  2:1128460/1‑137 (MQ=255)

                                                              aGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGgttt                                                              >  1:71411/1‑140 (MQ=255)

                                                               gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                             <  1:61047/140‑1 (MQ=255)

                                                                tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTAc                                                            >  1:17332/1‑140 (MQ=255)

                                                                tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGCCTGGCGGGTGGtttttt                                                            >  2:740548/1‑138 (MQ=255)

                                                                 ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                           <  2:1202800/140‑1 (MQ=255)

                                                                    ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                        <  1:976768/140‑1 (MQ=255)

                                                                    ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCGGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                        <  2:1010690/140‑1 (MQ=255)

                                                                        aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTGTCTGccca                                                    >  1:100282/1‑138 (MQ=255)

                                                                         tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAAc                                                   <  2:884855/140‑1 (MQ=255)

                                                                         tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCAAc                                                   >  1:540235/1‑140 (MQ=255)

                                                                              tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTTCTCTGCCAAACACgg                                              >  1:90775/1‑140 (MQ=255)

                                                                              tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACCCTTCCAACCAccg                                              >  2:1223883/1‑138 (MQ=255)

                                                                                   tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCAtt                                         >  2:633050/1‑139 (MQ=255)

                                                                                   tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCCGTGGTCTGGCGGGTGGTTTTTCTTTTCCAACCACgggcctt                                         >  2:404177/1‑135 (MQ=255)

                                                                                        aaaCAAGTATGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCAACCACGGGCCTGTCAtt                                    >  1:347488/1‑140 (MQ=255)

                                                                                              gTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCt                              <  2:1314459/140‑1 (MQ=255)

                                                                                                      tgggGCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGACGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATg                      <  1:1173071/140‑1 (MQ=255)

                                                                                                        gggCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTTCCAACCCCCGTCATTGCCTTTTTTTTGCGTAAtttt                    >  1:507918/1‑137 (MQ=255)

                                                                                                         ggCAATTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTTCTCTGCCCACCCCGGGCATGCCCTTTTTTTTGCGTAATGttc                   >  2:304390/1‑138 (MQ=255)

                                                                                                             aTTGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTTTCGGGGGGTTTTTTTTTGTCCACCACAGGCTTTTCATTTTTTTTTTGTAATGTTCGttt               >  2:1378352/1‑140 (MQ=255)

                                                                                                               tGACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc             <  1:1056568/140‑1 (MQ=255)

                                                                                                                gACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa            <  1:814802/140‑1 (MQ=255)

                                                                                                                gACACCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa            <  1:994366/140‑1 (MQ=255)

                                                                                                                   acCGTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTATTTGCCCACCACGGCCGTGTCCTTTTTTTTTTGTAAAGTCCCTTTTGAAgg         >  2:683325/1‑140 (MQ=255)

                                                                                                                      gTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTCCTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCTGTTTGCTAGGGGt      <  2:709587/140‑1 (MQ=255)

                                                                                                                      gTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt      <  1:414767/140‑1 (MQ=255)

                                                                                                                      gTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt      <  1:861712/140‑1 (MQ=255)

                                                                                                                      gTTGGCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCCGCGTAATGTCCGTTTGCAAGGGGt      <  1:1223883/140‑1 (MQ=255)

                                                                                                                         ggCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa   <  1:414649/140‑1 (MQ=255)

                                                                                                                          gCGACAAAGTTCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGACTGTGGTCTGGCGGGGGGTTTTTCTTTGCCAAACCCGGTTCTGCCTTTTTTTTTTTGTTATTTCCGTTTTTAAGGGGTgggt  >  2:355633/1‑138 (MQ=255)

TCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGAT  >  NC_000913/3404020‑3404281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A