Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I1 R1 61 131.2 4447032 99.1% 4407008 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,940,333 C→A A94S (GCA→TCA)  hsrA ← putative transporter HsrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,940,3330CA97.5% 421.7 / 4.0 121A94S (GCA→TCA) hsrAputative transporter HsrA
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (64/54);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 122 total aligned reads displayed.

CAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGC  >  NC_000913/3940202‑3940469
                                                                                                                                   |                                                                                                                                        
   tACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCagag                                                                                                                               <  1:1882346/140‑1 (MQ=255)
   aaCTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCagag                                                                                                                               <  2:837162/139‑1 (MQ=255)
      tGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAc                                                                                                                            <  2:41119/140‑1 (MQ=255)
      tGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAc                                                                                                                            >  2:820541/1‑140 (MQ=255)
      tGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAc                                                                                                                            <  2:773776/140‑1 (MQ=255)
      tGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAc                                                                                                                            <  2:1126032/140‑1 (MQ=255)
        gaagaaGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAccc                                                                                                                          >  1:893047/1‑140 (MQ=255)
        gaagaaGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAAccc                                                                                                                          >  1:607577/1‑140 (MQ=255)
               ttCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCAGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGa                                                                                                                   >  1:57280/1‑140 (MQ=255)
                tCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGaa                                                                                                                  <  1:161798/140‑1 (MQ=255)
                tCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGaa                                                                                                                  <  1:1587767/140‑1 (MQ=255)
                 cATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAAc                                                                                                                 <  1:1364759/140‑1 (MQ=255)
                    tACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGa                                                                                                              >  2:1007793/1‑140 (MQ=255)
                    tACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGa                                                                                                              <  2:1161848/140‑1 (MQ=255)
                        aGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCa                                                                                                          >  1:2081361/1‑140 (MQ=255)
                         ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCAc                                                                                                         >  2:193823/1‑140 (MQ=255)
                         ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCAc                                                                                                         >  2:505962/1‑140 (MQ=255)
                            tAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGc                                                                                                      <  2:2132874/140‑1 (MQ=255)
                            tAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGc                                                                                                      >  1:1329965/1‑140 (MQ=255)
                            tAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGc                                                                                                      <  2:1893684/140‑1 (MQ=255)
                            aaaGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGc                                                                                                      <  2:607577/139‑1 (MQ=255)
                             aaGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGTTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCa                                                                                                     <  2:2207155/140‑1 (MQ=255)
                                  cgcAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGt                                                                                                >  1:586862/1‑140 (MQ=255)
                                     aGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTaaa                                                                                             >  1:381223/1‑140 (MQ=255)
                                     aGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTaaa                                                                                             >  1:2018779/1‑140 (MQ=255)
                                             cAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGAc                                                                                     <  2:446701/140‑1 (MQ=255)
                                               gCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGAcgc                                                                                   >  2:469177/1‑140 (MQ=255)
                                               gCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGAcgc                                                                                   >  1:880006/1‑140 (MQ=255)
                                                ccGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGAcgct                                                                                  >  2:761267/1‑139 (MQ=255)
                                                ccGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGAcgcg                                                                                  <  1:1491611/140‑1 (MQ=255)
                                                 cGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGt                                                                                 >  1:1574086/1‑140 (MQ=255)
                                                 cGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGt                                                                                 >  1:634987/1‑140 (MQ=255)
                                                 cGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGt                                                                                 >  1:1145556/1‑140 (MQ=255)
                                                  gAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTa                                                                                <  2:586862/140‑1 (MQ=255)
                                                     cAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCg                                                                             >  1:841370/1‑140 (MQ=255)
                                                     cAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCg                                                                             <  1:914682/140‑1 (MQ=255)
                                                     cAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCg                                                                             >  1:54698/1‑140 (MQ=255)
                                                      aaCAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGa                                                                            <  2:1145556/140‑1 (MQ=255)
                                                          ggCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAATACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCg                                                                        >  2:475215/1‑140 (MQ=255)
                                                          ggCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCg                                                                        <  1:1568359/140‑1 (MQ=255)
                                                             atcatcatTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATc                                                                     <  2:893047/140‑1 (MQ=255)
                                                                atcatTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGc                                                                  >  2:2190422/1‑140 (MQ=255)
                                                                     tGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGcc                                                             >  1:1523314/1‑140 (MQ=255)
                                                                     tGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGcc                                                             >  1:120684/1‑140 (MQ=255)
                                                                      gcgcCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCa                                                            >  2:2116575/1‑140 (MQ=255)
                                                                       cgccgcCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCAt                                                           >  1:469665/1‑140 (MQ=255)
                                                                         ccgccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGcc                                                             >  2:1681049/1‑136 (MQ=255)
                                                                         ccgccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGcc                                                             <  1:1681049/136‑1 (MQ=255)
                                                                          cgccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCg                                                        <  1:2198342/140‑1 (MQ=255)
                                                                          cgccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCg                                                        >  2:1662133/1‑140 (MQ=255)
                                                                            ccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCt                                                      >  2:765225/1‑140 (MQ=255)
                                                                            ccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCt                                                      >  1:1031463/1‑140 (MQ=255)
                                                                            ccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCt                                                      >  1:773339/1‑140 (MQ=255)
                                                                            ccTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCt                                                      >  1:2031869/1‑140 (MQ=255)
                                                                               aTCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTAc                                                   <  2:284907/140‑1 (MQ=255)
                                                                               aTCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTAc                                                   <  2:589076/140‑1 (MQ=255)
                                                                               aTCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTAc                                                   <  1:2116575/140‑1 (MQ=255)
                                                                                   ccTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGa                                               <  1:1326656/140‑1 (MQ=255)
                                                                                    cTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGaa                                              <  1:765225/140‑1 (MQ=255)
                                                                                      gAATAACCCGGAAGACAACCAGCTGTGGTAGCTAATTGGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCCAGGGTAAAAATGCGACTCGTCCCGAAGCAATCGGCTAGCCATCCGCTTACCGGAATa                                            >  2:928894/1‑140 (MQ=255)
                                                                                      gAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATa                                            >  1:940800/1‑140 (MQ=255)
                                                                                       aaTAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATaa                                           >  2:1373903/1‑140 (MQ=255)
                                                                                       aaTAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATaa                                           >  2:1666163/1‑140 (MQ=255)
                                                                                        aTAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAg                                          >  2:813268/1‑140 (MQ=255)
                                                                                          aaCCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCa                                        >  1:986967/1‑140 (MQ=255)
                                                                                          aaCCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCa                                        <  1:761267/140‑1 (MQ=255)
                                                                                           aCCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCAt                                       >  2:1665179/1‑140 (MQ=255)
                                                                                            cccGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATc                                      >  1:1490229/1‑140 (MQ=255)
                                                                                            cccGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATc                                      >  1:62105/1‑140 (MQ=255)
                                                                                            cccGGAAGACAACCAGCTGTGGTAGCG‑ATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATcc                                     >  2:1465624/1‑139 (MQ=255)
                                                                                              cGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGGTAGCCATCCGCTTACCGGAATAAGCATCGc                                    <  2:120684/140‑1 (MQ=255)
                                                                                                 aaGACAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCAc                                 >  1:397726/1‑140 (MQ=255)
                                                                                                    aCAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAATGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGt                              <  2:940964/140‑1 (MQ=255)
                                                                                                    aCAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGt                              <  2:2031869/140‑1 (MQ=255)
                                                                                                    aCAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGt                              <  1:1007793/140‑1 (MQ=255)
                                                                                                    aCAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGt                              <  2:2201108/140‑1 (MQ=255)
                                                                                                    aCAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGt                              <  2:1264006/140‑1 (MQ=255)
                                                                                                     cAACCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTc                             >  1:822766/1‑140 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGCAGACAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGATAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            <  2:1993010/140‑1 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            <  2:2018779/140‑1 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            >  1:674814/1‑140 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            >  1:2217855/1‑140 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            >  1:1109493/1‑140 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            <  1:820541/140‑1 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            >  2:1413358/1‑140 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            <  1:193823/140‑1 (MQ=255)
                                                                                                      aaCCAGCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCa                            <  1:1834571/140‑1 (MQ=255)
                                                                                                           gCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGta                       <  1:874978/140‑1 (MQ=255)
                                                                                                           gCTGTGGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGta                       >  1:211276/1‑140 (MQ=255)
                                                                                                              gtgtTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATaa                    >  1:1356224/1‑140 (MQ=255)
                                                                                                              gtgGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCAAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATaa                    <  2:590150/140‑1 (MQ=255)
                                                                                                               tgGTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAAc                   >  2:1862898/1‑140 (MQ=255)
                                                                                                                ggTAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACt                  >  1:769077/1‑140 (MQ=255)
                                                                                                                  tAGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACtga                <  1:469177/140‑1 (MQ=255)
                                                                                                                   aGCGAATTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACtgat               <  2:1366711/140‑1 (MQ=255)
                                                                                                                     gcgaTTAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCAGCTAGCCATCCACTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACtgatga             <  1:1465624/137‑1 (MQ=255)
                                                                                                                     cGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACtgatga             <  1:1373903/140‑1 (MQ=255)
                                                                                                                         ttAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGc         <  1:1862898/140‑1 (MQ=255)
                                                                                                                         ttAGAAAGTGAGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGc         <  2:1109493/140‑1 (MQ=255)
                                                                                                                                gTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGCAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGc  >  2:2124035/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                        
CAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCACGGCAAGGGTAAAAATGCGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGC  >  NC_000913/3940202‑3940469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A