Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,096,922	T→A	Y176F (TAT→TTT)	rhaB ←	rhamnulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,096,922	0	T	A	99.1%	386.9 / ‑3.9	109	Y176F (TAT→TTT)	rhaB	rhamnulokinase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (59/49); total (60/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01

Only 100 of 110 total aligned reads displayed.

AATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAA  >  NC_000913/4096786‑4097058

aaTCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAAtt                                                                                                                                       <  2:1042340/140‑1 (MQ=255)

     aGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAg                                                                                                                                  >  2:1258533/1‑140 (MQ=255)

       tGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGtt                                                                                                                                >  2:715857/1‑140 (MQ=255)

            tATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCt                                                                                                                           >  1:684640/1‑140 (MQ=255)

             aTGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCtt                                                                                                                          >  2:381298/1‑140 (MQ=255)

                   ttACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGt                                                                                                                    <  2:1230848/140‑1 (MQ=255)

                    tACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTc                                                                                                                   >  2:540962/1‑140 (MQ=255)

                     actcGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTATCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTACCAGTTCATCTTGCCGGTCa                                                                                                                  >  1:641655/4‑140 (MQ=255)

                     aCCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCa                                                                                                                  >  2:1644474/1‑140 (MQ=255)

                     aCCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCa                                                                                                                  >  2:1716953/1‑140 (MQ=255)

                     aCCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCa                                                                                                                  >  2:1670225/1‑140 (MQ=255)

                     aCCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCa                                                                                                                  >  2:1092004/1‑140 (MQ=255)

                     aCCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCa                                                                                                                  >  2:980599/1‑140 (MQ=255)

                          gATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGa                                                                                                             >  2:144482/1‑140 (MQ=255)

                           aTGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGAt                                                                                                            >  2:1275399/1‑140 (MQ=255)

                           aTGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGAt                                                                                                            >  1:398521/1‑140 (MQ=255)

                           aTGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGAt                                                                                                            <  1:1670225/140‑1 (MQ=255)

                             gCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATaa                                                                                                          <  2:1303546/140‑1 (MQ=255)

                              cGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAAc                                                                                                         >  2:1430548/1‑140 (MQ=255)

                              cGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAAc                                                                                                         >  2:662099/1‑140 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCTAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTg                                                                                                       >  2:892207/1‑140 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGAt                                                                                                            <  1:604318/135‑1 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGAt                                                                                                            >  2:604318/1‑135 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTg                                                                                                       >  2:618311/1‑140 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTg                                                                                                       >  1:1093470/1‑140 (MQ=255)

                                tCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTg                                                                                                       >  1:739166/1‑140 (MQ=255)

                                       aCCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                                                                                <  2:1092284/140‑1 (MQ=255)

                                       aCCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                                                                                <  2:684640/140‑1 (MQ=255)

                                       aCCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                                                                                <  1:662099/140‑1 (MQ=255)

                                         cAAACCAGGCTTTGTTGGCCCCGCGCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATcc                                                                                              >  1:1526996/1‑140 (MQ=255)

                                                  cTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagc                                                                                     <  2:1597431/140‑1 (MQ=255)

                                                  cTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagc                                                                                     <  2:398521/140‑1 (MQ=255)

                                                   tttGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagca                                                                                    <  1:149535/140‑1 (MQ=255)

                                                    ttgttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagcag                                                                                   <  2:1093470/140‑1 (MQ=255)

                                                      gttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCagat                                                                                 >  2:677016/1‑139 (MQ=255)

                                                      gttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCagag                                                                                 <  1:1082981/140‑1 (MQ=255)

                                                      gttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCagag                                                                                 >  1:1156576/1‑140 (MQ=255)

                                                      gttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCagag                                                                                 <  1:925560/140‑1 (MQ=255)

                                                           ccccGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGa                                                                            >  2:897804/1‑140 (MQ=255)

                                                             ccGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGc                                                                          <  1:144482/140‑1 (MQ=255)

                                                              cgttCCATGCCAGTAGCGACTCGTCTCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAGATTACCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCa                                                                         <  2:641655/137‑1 (MQ=255)

                                                               gCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCaa                                                                        <  1:1536385/140‑1 (MQ=255)

                                                               gCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCaa                                                                        >  2:103608/1‑140 (MQ=255)

                                                               gCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCaa                                                                        >  1:639409/1‑140 (MQ=255)

                                                                cTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAt                                                                       >  1:952235/1‑140 (MQ=255)

                                                                cTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAt                                                                       >  1:667792/1‑140 (MQ=255)

                                                                cTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAt                                                                       >  1:632594/1‑140 (MQ=255)

                                                                   cACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtg                                                                    <  1:874833/140‑1 (MQ=255)

                                                                     cGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtgtg                                                                  >  2:862256/1‑140 (MQ=255)

                                                                     cGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtgtg                                                                  >  1:1496301/1‑140 (MQ=255)

                                                                        cAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGaa                                                               <  1:618311/140‑1 (MQ=255)

                                                                           tAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATat                                                            >  2:781350/1‑139 (MQ=255)

                                                                           tAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATaa                                                            >  2:1192699/1‑140 (MQ=255)

                                                                           tAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATaa                                                            >  2:736748/1‑140 (MQ=255)

                                                                           tAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATaa                                                            >  1:1066541/1‑140 (MQ=255)

                                                                                aCTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCa                                                       >  1:1109270/1‑140 (MQ=255)

                                                                                aCTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCa                                                       <  1:715857/140‑1 (MQ=255)

                                                                                aCTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCa                                                       >  2:1569196/1‑140 (MQ=255)

                                                                                    gTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgtt                                                   <  2:667792/140‑1 (MQ=255)

                                                                                    gTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgtt                                                   <  2:632594/140‑1 (MQ=255)

                                                                                    gTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgtt                                                   <  2:1496301/140‑1 (MQ=255)

                                                                                      cccAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgttgt                                                 <  1:1644474/140‑1 (MQ=255)

                                                                                      cccAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgttgt                                                 <  2:933814/140‑1 (MQ=255)

                                                                                        cAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGttgttg                                               <  1:1578963/140‑1 (MQ=255)

                                                                                                gCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCa                                       >  1:920487/1‑140 (MQ=255)

                                                                                                gCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCa                                       >  2:364323/1‑140 (MQ=255)

                                                                                                 cTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAg                                      >  1:970173/1‑140 (MQ=255)

                                                                                                  tattgatattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAgc                                     <  1:677016/140‑1 (MQ=255)

                                                                                                     tgatattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgca                                  <  1:897804/140‑1 (MQ=255)

                                                                                                     tgatattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgca                                  <  2:1526996/140‑1 (MQ=255)

                                                                                                     tgatattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgca                                  >  2:846440/1‑140 (MQ=255)

                                                                                                       atattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgcacg                                <  1:103608/140‑1 (MQ=255)

                                                                                                        tattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgcacgc                               <  2:1066541/140‑1 (MQ=255)

                                                                                                        tattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgcacgc                               <  2:920487/140‑1 (MQ=255)

                                                                                                        tattgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgcacgc                               <  2:639409/140‑1 (MQ=255)

                                                                                                         attgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGcgcacgca                              >  2:876433/1‑140 (MQ=255)

                                                                                                          ttgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCaa                             <  1:1258533/140‑1 (MQ=255)

                                                                                                          ttgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCaa                             <  1:1275399/140‑1 (MQ=255)

                                                                                                          ttgaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCaa                             <  2:228025/140‑1 (MQ=255)

                                                                                                            gaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACt                           >  1:1091623/1‑140 (MQ=255)

                                                                                                            gaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACt                           >  2:173099/1‑140 (MQ=255)

                                                                                                            gaCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACt                           >  2:1385186/1‑140 (MQ=255)

                                                                                                             aCCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTg                          >  1:1579168/1‑140 (MQ=255)

                                                                                                             aCCAGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTg                          <  2:1156576/140‑1 (MQ=255)

                                                                                                                aGTTGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATa                       <  1:781350/140‑1 (MQ=255)

                                                                                                                  ttGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATaaa                     <  1:1294963/140‑1 (MQ=255)

                                                                                                                  ttGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATaaa                     >  2:489274/1‑140 (MQ=255)

                                                                                                                  ttGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATaaa                     <  1:1569196/140‑1 (MQ=255)

                                                                                                                  ttGCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATaaa                     <  1:892207/140‑1 (MQ=255)

                                                                                                                    gCGTGGTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGc                   >  2:1056400/1‑140 (MQ=255)

                                                                                                                         gTCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTAtt              <  1:266099/140‑1 (MQ=255)

                                                                                                                          tCGTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTg             <  1:489274/140‑1 (MQ=255)

                                                                                                                            gTGGCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGaa           >  2:74291/1‑140 (MQ=255)

                                                                                                                               gCGTTGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAggg        <  1:350558/140‑1 (MQ=255)

                                                                                                                                   tGGTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGa    <  1:618667/140‑1 (MQ=255)

                                                                                                                                    ggTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGaa   >  1:203635/1‑140 (MQ=255)

                                                                                                                                    ggTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGaa   >  1:241555/1‑140 (MQ=255)

                                                                                                                                    ggTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGaa   >  2:1337176/1‑140 (MQ=255)

                                                                                                                                    ggTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGaa   >  2:1207041/1‑140 (MQ=255)

                                                                                                                                     gTAAATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGaaa  <  1:1534294/140‑1 (MQ=255)

AATCCAGTGACCTATGACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAA  >  NC_000913/4096786‑4097058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A