Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,763	C→A	A185A (GCG→GCT)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,763	0	C	A	100.0%	219.8 / NA	62	A185A (GCG→GCT)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (31/31); total (31/31)

TTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGCGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTT  >  NC_000913/4147626‑4147898

ttCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAgagc                                                                                                                                       >  2:1079584/1‑140 (MQ=255)

ttCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAgagc                                                                                                                                       >  1:1460082/1‑140 (MQ=255)

ttCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAgagc                                                                                                                                       >  2:402692/1‑140 (MQ=255)

 tCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGagcg                                                                                                                                      <  2:256483/140‑1 (MQ=255)

  cAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCgg                                                                                                                                     <  2:1292559/140‑1 (MQ=255)

     tAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAgg                                                                                                                                  >  1:930516/1‑140 (MQ=255)

       aCGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCa                                                                                                                                >  1:1078979/1‑140 (MQ=255)

        cGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCAt                                                                                                                               >  2:1591517/1‑140 (MQ=255)

            ttAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCg                                                                                                                           <  2:254845/140‑1 (MQ=255)

              aGCGGGCTCCAGTCGCGTGTGAATCAGGTAGTCGTTAAAACCAATGGCCATCGTTGTTTGAAACAGGTGCGAGAGGTAAAGGGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGtt                                                                                                                         <  2:1522382/140‑1 (MQ=255)

                 gTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTtcgt                                                                                                                      <  2:58973/140‑1 (MQ=255)

                 gTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTtcgt                                                                                                                      <  2:1460082/140‑1 (MQ=255)

                    cTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGa                                                                                                                   >  1:303508/1‑140 (MQ=255)

                      ccAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGata                                                                                                                 <  2:303508/140‑1 (MQ=255)

                         gTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGTGGAGGCATAGCGTTCGTCGATATaa                                                                                                              >  2:57260/1‑140 (MQ=255)

                               tgtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGa                                                                                                        >  2:831434/1‑140 (MQ=255)

                               tgtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGa                                                                                                        >  2:652957/1‑140 (MQ=255)

                               tgtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGa                                                                                                        >  2:1714442/1‑140 (MQ=255)

                                gtgATTCAGGTATTCGTTAAAACCGATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGCAATTTGGCGAAATATAACACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGaa                                                                                                       <  1:791782/140‑1 (MQ=255)

                                gtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGaa                                                                                                       <  2:435851/140‑1 (MQ=255)

                                gtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGaa                                                                                                       <  1:652957/140‑1 (MQ=255)

                                gtgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGaa                                                                                                       <  2:508818/140‑1 (MQ=255)

                                 tgATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAAt                                                                                                      <  2:1051620/140‑1 (MQ=255)

                                    ttCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGCGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGc                                                                                                   >  1:825652/1‑140 (MQ=255)

                                     tCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCt                                                                                                  <  2:1074496/140‑1 (MQ=255)

                                        ggTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCa                                                                                               >  1:501390/1‑140 (MQ=255)

                                        ggTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCa                                                                                               >  1:363415/1‑140 (MQ=255)

                                          tATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCaaa                                                                                             <  1:876162/140‑1 (MQ=255)

                                          tATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCaaa                                                                                             >  2:1105532/1‑140 (MQ=255)

                                             tCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAg                                                                                          >  2:954408/1‑140 (MQ=255)

                                                 tAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGcc                                                                                      >  1:208313/1‑140 (MQ=255)

                                                             cccccGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAg                                                                          <  1:954408/140‑1 (MQ=255)

                                                             cccccGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAg                                                                          <  1:1714442/140‑1 (MQ=255)

                                                               cccGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGc                                                                        <  1:441461/140‑1 (MQ=255)

                                                                 cGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCgg                                                                      >  1:901701/1‑140 (MQ=255)

                                                                 cGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCgg                                                                      >  2:1348543/1‑140 (MQ=255)

                                                                  gTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGt                                                                     <  1:1079584/140‑1 (MQ=255)

                                                                                gTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGc                                                       >  2:250884/1‑140 (MQ=255)

                                                                                 tGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGcc                                                      <  1:1348543/140‑1 (MQ=255)

                                                                                 tGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGcc                                                      <  2:177295/140‑1 (MQ=255)

                                                                                    gagaGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAg                                                   >  1:482677/1‑140 (MQ=255)

                                                                                      gagGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCa                                                 <  1:250884/140‑1 (MQ=255)

                                                                                        ggTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCaaa                                               >  1:1328005/1‑140 (MQ=255)

                                                                                        ggTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCaaa                                               >  2:755624/1‑140 (MQ=255)

                                                                                         gTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAAt                                              >  2:1446061/1‑140 (MQ=255)

                                                                                               tGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGTGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGc                                        <  1:57260/140‑1 (MQ=255)

                                                                                               tGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGc                                        <  2:825652/140‑1 (MQ=255)

                                                                                               tGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGc                                        <  2:482677/140‑1 (MQ=255)

                                                                                               tGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGc                                        <  1:1591517/140‑1 (MQ=255)

                                                                                                   gAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGtt                                    >  2:1075040/1‑139 (MQ=255)

                                                                                                    aaaTATAAAACGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTgg                                   >  1:448539/1‑140 (MQ=255)

                                                                                                             aCGCCTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAgg                          <  2:448539/140‑1 (MQ=255)

                                                                                                                 cTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTgg                      <  2:363415/140‑1 (MQ=255)

                                                                                                                 cTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTgg                      >  2:69468/1‑140 (MQ=255)

                                                                                                                 cTGTGCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTgg                      <  2:501390/140‑1 (MQ=255)

                                                                                                                    tgCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTTAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCATGCTGGcca                   >  2:78249/1‑138 (MQ=255)

                                                                                                                    tgCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGcgg                   >  2:1720653/1‑139 (MQ=255)

                                                                                                                    tgCAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCCGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGcgc                   >  1:775954/1‑139 (MQ=255)

                                                                                                                      cAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGTAGGCTGGcgact                 >  2:579309/1‑139 (MQ=255)

                                                                                                                      cAACAGATTCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGcgacg                 <  1:553437/140‑1 (MQ=255)

                                                                                                                              tCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGcc          <  1:221737/139‑1 (MQ=255)

                                                                                                                              tCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGcc          >  2:221737/1‑139 (MQ=255)

                                                                                                                              tCGCGGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCt         >  2:407979/1‑140 (MQ=255)

                                                                                                                               cgcgGGTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCtt        <  2:486672/140‑1 (MQ=255)

                                                                                                                                  gggTAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGcc     <  1:755624/140‑1 (MQ=255)

                                                                                                                                     tAAGAGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTAGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGtt  >  2:1038705/1‑140 (MQ=255)

TTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCCCCGTTTTTTGAAACAGGTGCGAGAGGTAATTTGGCGAAATATAAAACGCCTGTGCAACAGATTCGCGGGTAAGCGCGGAGGCATAGCGTTCGTCGATATAATCGCGAATAGCTTCAAATAGTGCCTGGCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTT  >  NC_000913/4147626‑4147898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A