Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,464,988	G→T	P398Q (CCG→CAG)	treB ←	trehalose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,464,988	0	G	T	100.0%	285.2 / NA	80	P398Q (CCG→CAG)	treB	trehalose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (41/39); total (41/39)

CGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCACGGCGG  >  NC_000913/4464852‑4465120

cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGa                                                                                                                                   >  1:75373/1‑140 (MQ=255)

cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGa                                                                                                                                   >  2:1240201/1‑140 (MQ=255)

cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGa                                                                                                                                   >  2:383403/1‑140 (MQ=255)

cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGa                                                                                                                                   >  2:655593/1‑140 (MQ=255)

  cAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAg                                                                                                                                 <  1:346545/140‑1 (MQ=255)

   aaaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGc                                                                                                                                <  1:1234249/140‑1 (MQ=255)

    aaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCg                                                                                                                               <  2:75373/140‑1 (MQ=255)

    aaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCg                                                                                                                               <  1:615642/140‑1 (MQ=255)

    aaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCg                                                                                                                               <  1:219723/140‑1 (MQ=255)

     acacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGa                                                                                                                              >  2:1703099/1‑140 (MQ=255)

     acacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGa                                                                                                                              >  2:827308/1‑140 (MQ=255)

      cacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGat                                                                                                                             <  2:473425/140‑1 (MQ=255)

         cTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATAtt                                                                                                                          >  2:915869/1‑140 (MQ=255)

          tGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATAttt                                                                                                                         >  1:1368934/1‑140 (MQ=255)

               gTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGt                                                                                                                    <  1:1703099/140‑1 (MQ=255)

               gTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGt                                                                                                                    <  1:827308/140‑1 (MQ=255)

               gTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGt                                                                                                                    <  2:531563/140‑1 (MQ=255)

                tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGtt                                                                                                                   >  1:1546239/1‑140 (MQ=255)

                 aGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTg                                                                                                                  >  2:1356178/1‑140 (MQ=255)

                   cTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGAt                                                                                                                >  2:421939/1‑140 (MQ=255)

                   cTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGAt                                                                                                                >  2:128533/1‑140 (MQ=255)

                    tCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATg                                                                                                               <  2:1374447/140‑1 (MQ=255)

                     cGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGc                                                                                                              <  2:545745/140‑1 (MQ=255)

                      ggTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGcc                                                                                                             >  2:824563/1‑140 (MQ=255)

                       gTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCg                                                                                                            >  1:1334101/1‑140 (MQ=255)

                           aaTCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACa                                                                                                        >  2:1315477/1‑140 (MQ=255)

                              cGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTg                                                                                                     <  1:421939/140‑1 (MQ=255)

                                agagaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCa                                                                                                   >  2:334003/1‑140 (MQ=255)

                                agagaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCa                                                                                                   >  1:1147670/1‑140 (MQ=255)

                                agagaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCa                                                                                                   <  2:1089057/140‑1 (MQ=255)

                                agagaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCa                                                                                                   >  2:945401/1‑140 (MQ=255)

                                agagaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCAGCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCa                                                                                                   <  2:1373540/140‑1 (MQ=255)

                                  agaATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAgg                                                                                                 <  2:1368934/140‑1 (MQ=255)

                                    agcacctggCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCt                                                                                               <  1:728756/133‑1 (MQ=255)

                                    aaTTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCt                                                                                               <  2:1276331/140‑1 (MQ=255)

                                      ttCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCa                                                                                             <  1:1624442/140‑1 (MQ=255)

                                        cccGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt                                                                                           <  1:1356178/140‑1 (MQ=255)

                                        cccGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt                                                                                           <  2:229540/140‑1 (MQ=255)

                                        cccGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt                                                                                           <  2:1029000/140‑1 (MQ=255)

                                        cccGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt                                                                                           <  1:1670429/140‑1 (MQ=255)

                                              aGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGAcccc                                                                                     >  1:434156/1‑140 (MQ=255)

                                                  cgccTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc                                                                                 <  2:1376057/140‑1 (MQ=255)

                                                  cgccTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc                                                                                 <  1:334003/140‑1 (MQ=255)

                                                  cgccTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc                                                                                 <  2:1217190/140‑1 (MQ=255)

                                                  cgccTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc                                                                                 <  1:655593/140‑1 (MQ=255)

                                                  cgacTACGCAGATGCCATTCGCAAGAACGACGTGCAGGACGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGACGTACATTGCAGGCTCAGTGACCCCAAGc                                                                                 <  2:51358/137‑1 (MQ=255)

                                                      tACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAgg                                                                             <  2:471153/140‑1 (MQ=255)

                                                                ccATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGc                                                                   <  1:449786/140‑1 (MQ=255)

                                                                  aTTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCgg                                                                 <  2:434156/140‑1 (MQ=255)

                                                                  aTTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCgg                                                                 >  1:584022/1‑140 (MQ=255)

                                                                   ttCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc                                                                >  2:191053/1‑140 (MQ=255)

                                                                   ttCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc                                                                >  2:1000632/1‑140 (MQ=255)

                                                                   ttCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc                                                                <  1:1315477/140‑1 (MQ=255)

                                                                    tCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggca                                                               <  2:699369/140‑1 (MQ=255)

                                                                    tCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggca                                                               <  1:1240201/140‑1 (MQ=255)

                                                                             cGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag                                                      <  1:21957/140‑1 (MQ=255)

                                                                             cGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag                                                      >  1:550168/1‑140 (MQ=255)

                                                                                  ttCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtctc                                                 >  1:679149/1‑140 (MQ=255)

                                                                                  ttCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtctc                                                 >  1:1667449/1‑140 (MQ=255)

                                                                                    cAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc                                               <  1:383403/140‑1 (MQ=255)

                                                                                        ccGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTc                                           >  1:436234/1‑140 (MQ=255)

                                                                                            aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg                                       >  2:224146/1‑140 (MQ=255)

                                                                                            aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg                                       >  2:399172/1‑140 (MQ=255)

                                                                                            aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCAAGCAGCACTCGCGATAATtg                                       >  2:802448/1‑140 (MQ=255)

                                                                                                 aaTCCTGCCAGTCCAGATCCGATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTGCATGGCAGGCTCAGTGACCCCAAGCCTGGCGGAGATAGCGGCAGGCACGGAGGTCTCGCGTTCGTTGTGCtt                                  <  2:436234/140‑1 (MQ=255)

                                                                                                        ccagaccagaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGCCTg                           >  2:1184741/1‑140 (MQ=255)

                                                                                                          agaccagaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGa                         >  1:427642/1‑140 (MQ=255)

                                                                                                             ccagaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAAt                      >  1:1298194/1‑140 (MQ=255)

                                                                                                             ccagaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAAt                      >  1:672048/1‑140 (MQ=255)

                                                                                                              cagaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATg                     >  1:905673/1‑140 (MQ=255)

                                                                                                               agaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGa                    <  1:399172/140‑1 (MQ=255)

                                                                                                               agaACCAATCATCGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGa                    <  1:945401/140‑1 (MQ=255)

                                                                                                                      atcatcGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGcc             >  2:689164/1‑140 (MQ=255)

                                                                                                                      atcatcGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGcc             >  2:1298922/1‑140 (MQ=255)

                                                                                                                      atcatcGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGcc             <  1:1292736/140‑1 (MQ=255)

                                                                                                                         atcGCGCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTAt          <  2:550168/140‑1 (MQ=255)

                                                                                                                           cgcgCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATcc        >  2:434128/1‑139 (MQ=255)

                                                                                                                           cgcgCACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACCGAGATCTCGCGCTCATTGTTCTTGCGGCTGGGAATGGTATTGACTAttc        >  2:1643677/1‑138 (MQ=255)

                                                                                                                           agcgcACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTAAGTGGCCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCa        <  2:1405652/139‑1 (MQ=255)

                                                                                                                             cgcACAGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCAcg      >  2:1139657/1‑140 (MQ=255)

                                                                                                                               cacaGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCAcggc    >  1:685192/1‑140 (MQ=255)

                                                                                                                                acaGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCAcggcg   <  2:695979/140‑1 (MQ=255)

                                                                                                                                 caGCATCTGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCAcggcgg  >  1:149156/1‑140 (MQ=255)

CGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGATAATGCCTATCACGGCGG  >  NC_000913/4464852‑4465120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A