Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,029,329	C→T	E535K (GAG→AAG)	ygfT ←	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,029,329	0	C	T	100.0%	169.8 / NA	50	E535K (GAG→AAG)	ygfT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/30); total (20/30)

AATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTGCGCGAGC  >  NC_000913/3029198‑3029460

aaTTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATgg                                                                                                                             <  1:1133461/140‑1 (MQ=255)

aaTTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATgg                                                                                                                             <  1:1160845/140‑1 (MQ=255)

    ccGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGt                                                                                                                         <  2:1164282/140‑1 (MQ=255)

          cccTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAAt                                                                                                                   <  1:275847/140‑1 (MQ=255)

             tGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAg                                                                                                                >  1:889719/1‑140 (MQ=255)

                  ccACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCa                                                                                                           <  2:34504/140‑1 (MQ=255)

                  ccACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCa                                                                                                           <  1:1057326/140‑1 (MQ=255)

                  acACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCa                                                                                                           <  2:508338/139‑1 (MQ=255)

                   cACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCAc                                                                                                          <  2:340512/140‑1 (MQ=255)

                    aCGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCCACCGGGAGAGGACGACGGGGACCCACCCGCCCCGGCTTAACCACGGCGGGACGAATCAGGCCCAcc                                                                                                         >  2:376042/1‑140 (MQ=255)

                     cGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCg                                                                                                        >  2:100182/1‑140 (MQ=255)

                          tggcatgCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGtt                                                                                                   <  1:259896/140‑1 (MQ=255)

                          tggcatgCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGtt                                                                                                   <  1:495283/140‑1 (MQ=255)

                            gcatgCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTTCCCATGGCGGGACGAATCAGGCCCACCGCAGTTaa                                                                                                 >  2:376299/1‑140 (MQ=255)

                                gcgcCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGc                                                                                             >  1:634972/1‑140 (MQ=255)

                                gcgcCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGc                                                                                             >  1:935301/1‑140 (MQ=255)

                                            cAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTca                                                                                 <  1:100182/140‑1 (MQ=255)

                                             aaaGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTcac                                                                                <  2:535176/140‑1 (MQ=255)

                                             aaaGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTcac                                                                                <  2:889719/140‑1 (MQ=255)

                                             aaaGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTcac                                                                                <  2:1058828/140‑1 (MQ=255)

                                                ggCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAg                                                                             <  1:852214/140‑1 (MQ=255)

                                                          aGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTg                                                                   <  2:1281876/140‑1 (MQ=255)

                                                           gAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGc                                                                  >  2:306486/1‑140 (MQ=255)

                                                            aaCATCGGCGGGCAATTCAAACTCTGAACCCGCTACTGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCg                                                                 >  1:951419/1‑140 (MQ=255)

                                                            aaCATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCTTGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCg                                                                 >  2:716503/1‑140 (MQ=255)

                                                            aaCATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCAGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCg                                                                 >  1:913758/1‑140 (MQ=255)

                                                             aCATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCgg                                                                <  1:539758/140‑1 (MQ=255)

                                                                 cggcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGa                                                            >  1:319270/1‑140 (MQ=255)

                                                                 cggcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCCGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGa                                                            >  1:1277050/1‑140 (MQ=255)

                                                                 cggcggGCAATTCAAAATCTGAACCCGCTACCGGAAGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGa                                                            >  2:749383/1‑140 (MQ=255)

                                                                  ggcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGaa                                                           >  1:1104719/1‑140 (MQ=255)

                                                                  ggcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGaa                                                           <  2:655821/140‑1 (MQ=255)

                                                                  ggcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCCTACCCATGGCGGTACGAATCAGACCCACCGCAGTTAAACGCCCATCTTCCTCACAAGCGAGATAATTCGGTTTaa                                                           >  2:758892/1‑140 (MQ=255)

                                                                   gcggGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAAc                                                          >  1:261016/1‑140 (MQ=255)

                                                                            tCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTg                                                 <  2:96488/140‑1 (MQ=255)

                                                                             aaaaCTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGa                                                <  2:261016/139‑1 (MQ=255)

                                                                                 ctctGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACt                                            <  1:306486/140‑1 (MQ=255)

                                                                                    tGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGa                                         <  1:507467/140‑1 (MQ=255)

                                                                                     gAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGTGGTTGAACATTGAACTGAAACTCGac                                        >  2:1318382/1‑140 (MQ=255)

                                                                                      aaCCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGaca                                       >  1:1269235/1‑140 (MQ=255)

                                                                                      aaCCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGaca                                       >  1:941351/1‑140 (MQ=255)

                                                                                               ccGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTcg                              <  2:1365609/140‑1 (MQ=255)

                                                                                                cGGACGAGGACGACGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTcgc                             <  2:935301/140‑1 (MQ=255)

                                                                                                cGGACGAGGACGACGGCGACCATCCAGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTcgc                             <  2:913758/140‑1 (MQ=255)

                                                                                                  gacgaggacgaCGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACTCCTTCCTcgcgc                           <  1:758892/140‑1 (MQ=255)

                                                                                                    cgaggacgaCGGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCgcgcgc                         >  1:954617/1‑140 (MQ=255)

                                                                                                         acgacgGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTaa                    >  1:287907/1‑140 (MQ=255)

                                                                                                         acgacgGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTaa                    <  2:938840/140‑1 (MQ=255)

                                                                                                             cgGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAaccac                >  2:466544/1‑140 (MQ=255)

                                                                                                             cgGCGACCATCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAaccac                <  2:941351/140‑1 (MQ=255)

                                                                                                                      tCCGGCCCCGGCTTACCCGTGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTgcg       <  1:1261672/140‑1 (MQ=255)

                                                                                                                      tCCGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTgcg       >  2:294394/1‑140 (MQ=255)

                                                                                                                      accGGCCCCGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTgcg       <  2:493106/139‑1 (MQ=255)

                                                                                                                           ccccGGCTTACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTGCGCGAGc  <  2:951419/140‑1 (MQ=255)

AATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTACGAATCAGGCCCACCGCAGTTAAACGCCCATCTTCGTCACAAGCGATATATTGCGGTTGAACATTGAACTGAAACTCGACACCTTCCTCGCGCGCATTAACCACCTCTTTGCGCGAGC  >  NC_000913/3029198‑3029460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A