Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F48 I1 R1 83 81.6 2760494 99.1% 2735649 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,254,160 C→A D19Y (GAT→TAT)  yhaJ ← DNA‑binding transcriptional activator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,254,1600CA100.0% 197.2 / NA 56D19Y (GAT→TAT) yhaJDNA‑binding transcriptional activator YhaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/30);  total (26/30)

GTGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATCGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAGG  >  NC_000913/3254024‑3254296
                                                                                                                                        |                                                                                                                                        
gTGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGcgatagat                                                                                                                                       <  1:1367463/140‑1 (MQ=255)
 tGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCgatagatc                                                                                                                                      >  2:212401/1‑140 (MQ=255)
  gAATTTGGTCAGAGGGCCCGAGCGGTCAAACAGCACCACATCCCGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGAACGCGGCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGatagatcg                                                                                                                                     <  2:633037/140‑1 (MQ=255)
  gAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGGGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGatagatcg                                                                                                                                     <  2:852986/140‑1 (MQ=255)
  gAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGatagatcg                                                                                                                                     <  2:1354264/140‑1 (MQ=255)
  gAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGatagatcg                                                                                                                                     <  1:1302152/140‑1 (MQ=255)
   aaTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGc                                                                                                                                    >  1:1030627/1‑140 (MQ=255)
         gTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCAt                                                                                                                              <  1:612683/140‑1 (MQ=255)
         gTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCAt                                                                                                                              >  1:682906/1‑140 (MQ=255)
          tACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATa                                                                                                                             <  2:261001/140‑1 (MQ=255)
               tGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCg                                                                                                                        >  2:890298/1‑140 (MQ=255)
                  cccGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAg                                                                                                                     >  1:573142/1‑140 (MQ=255)
                           tCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAg                                                                                                            >  2:533705/1‑140 (MQ=255)
                               aCAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGtt                                                                                                        <  2:1341740/140‑1 (MQ=255)
                               aCAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGtt                                                                                                        <  1:1089403/140‑1 (MQ=255)
                                cAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCAATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTa                                                                                                       <  1:1249947/140‑1 (MQ=255)
                                   caccacATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATg                                                                                                    >  1:118508/1‑140 (MQ=255)
                                     ccacATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGTAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGcc                                                                                                  >  1:1340011/1‑140 (MQ=255)
                                     ccacATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGcc                                                                                                  >  2:219969/1‑140 (MQ=255)
                                     ccacATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGcc                                                                                                  >  2:1149550/1‑140 (MQ=255)
                                             aGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCctttcttt                                                                                          >  2:106640/1‑140 (MQ=255)
                                             aGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCctttcttt                                                                                          >  2:565764/1‑140 (MQ=255)
                                              gCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTg                                                                                         >  2:1057348/1‑140 (MQ=255)
                                               ctctTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGTAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTgg                                                                                        <  2:1340011/140‑1 (MQ=255)
                                               ctctTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTgg                                                                                        <  2:1009991/140‑1 (MQ=255)
                                                tcttctTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGc                                                                                       <  2:873996/140‑1 (MQ=255)
                                                tcttctTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGc                                                                                       >  1:1183461/1‑140 (MQ=255)
                                                  ttcttcCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCa                                                                                     >  2:685712/1‑140 (MQ=255)
                                                    cttcCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCAtt                                                                                   >  2:633084/1‑140 (MQ=255)
                                                       ccAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCg                                                                                <  2:1225289/140‑1 (MQ=255)
                                                         aGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGtt                                                                              >  1:455916/1‑140 (MQ=255)
                                                                  aTGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAg                                                                     <  2:86642/140‑1 (MQ=255)
                                                                    ggTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATACGCCGCCGCCGCTAAACTGCCCCTGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGa                                                                   >  2:634656/1‑140 (MQ=255)
                                                                           cTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGa                                                            <  1:895257/140‑1 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATACCCCGTAGAGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  2:695268/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       <  1:157006/140‑1 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  2:234530/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  2:1164377/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  2:440775/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       <  2:573142/140‑1 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  1:1080446/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       <  1:791479/140‑1 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       >  1:747225/1‑140 (MQ=255)
                                                                                tGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATa                                                       <  1:633084/140‑1 (MQ=255)
                                                                                   ggAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCg                                                    <  1:212401/140‑1 (MQ=255)
                                                                                    gAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCgg                                                   <  1:533705/140‑1 (MQ=255)
                                                                                             cgTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAatat                                          <  1:890298/140‑1 (MQ=255)
                                                                                             cgTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAatat                                          <  2:257449/140‑1 (MQ=255)
                                                                                             cgTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAatat                                          <  1:284226/140‑1 (MQ=255)
                                                                                                cccAGCTCAACCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTg                                       <  2:1098007/140‑1 (MQ=255)
                                                                                                  cAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGc                                     <  1:985073/140‑1 (MQ=255)
                                                                                                   aGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCt                                    <  1:1174262/140‑1 (MQ=255)
                                                                                                          ccgccgccgccgCAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAAtt                             >  2:813594/1‑139 (MQ=255)
                                                                                                            gccgccgccgcAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTa                           <  1:565764/140‑1 (MQ=255)
                                                                                                                ccgccgcAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGt                       >  2:839215/1‑140 (MQ=255)
                                                                                                                 cgccgcAAAACTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTc                      <  1:813594/140‑1 (MQ=255)
                                                                                                                         aaCTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCt              <  1:1164377/140‑1 (MQ=255)
                                                                                                                         aaCTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCt              <  2:1183461/140‑1 (MQ=255)
                                                                                                                         aaCTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCt              <  2:118508/140‑1 (MQ=255)
                                                                                                                          aCTGCCCCGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCtt             <  1:485423/140‑1 (MQ=255)
                                                                                                                                ccGGCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATa       <  2:347295/140‑1 (MQ=255)
                                                                                                                                   gCGATAGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATaaaa    >  1:1299994/1‑140 (MQ=255)
                                                                                                                                    cgatagatCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAg   <  1:685712/140‑1 (MQ=255)
                                                                                                                                    cgatagatCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAg   <  1:1057348/140‑1 (MQ=255)
                                                                                                                                    cgatagatCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAg   <  2:1080446/140‑1 (MQ=255)
                                                                                                                                    cgatagatCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAg   <  1:1149550/140‑1 (MQ=255)
                                                                                                                                    cgatagatCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAg   <  2:250586/140‑1 (MQ=255)
                                                                                                                                     gatagatcGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAgg  <  1:219969/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATCGATCGCATCCATAACCCGTAGTGCTTCCAGCGTTAATGCCCTTTCTTTGGCCATTTCGTTCTCATTCAGGAAATTTGAACATACCGGGCAGAATATCTGGCTAACAATGTAGCGTCCAGCCCCTTACCATAAAAGG  >  NC_000913/3254024‑3254296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A