Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F48 I1 R1 83 81.6 2760494 99.1% 2735649 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,255,674 C→A T326T (ACG→ACT)  cyuA ← putative L‑cysteine desulfidase CyuA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,255,6740CA97.6% 281.0 / ‑3.9 82T326T (ACG→ACT) cyuAputative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (41/39);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGCAGCTGTTCGACGCACCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTCGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAGGCATTG  >  NC_000913/3255538‑3255810
                                                                                                                                        |                                                                                                                                        
tCGCGCAGCTGTTCGACGCACCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGtt                                                                                                                                       <  1:585790/140‑1 (MQ=255)
     cAGCTGTTCGACGCACCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGc                                                                                                                                  >  2:224633/1‑140 (MQ=255)
           ttCGACGCACCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAg                                                                                                                            <  1:530020/140‑1 (MQ=255)
                   aCCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACa                                                                                                                    <  2:1246346/140‑1 (MQ=255)
                   aCCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACa                                                                                                                    >  2:568291/1‑140 (MQ=255)
                     cATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACaaa                                                                                                                  >  2:345628/1‑140 (MQ=255)
                     cATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACaaa                                                                                                                  >  1:523699/1‑140 (MQ=255)
                       tCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAAcg                                                                                                                <  2:334942/140‑1 (MQ=255)
                         gCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAAcgcg                                                                                                              <  2:536637/140‑1 (MQ=255)
                         gCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAAcgcg                                                                                                              <  1:137599/140‑1 (MQ=255)
                           aaaTCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGt                                                                                                            <  1:224633/140‑1 (MQ=255)
                           aaaTCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGt                                                                                                            <  2:596441/140‑1 (MQ=255)
                             aTCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTaa                                                                                                          <  2:507650/140‑1 (MQ=255)
                             aTCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTaa                                                                                                          <  1:582259/140‑1 (MQ=255)
                               cATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACt                                                                                                        <  1:1044066/140‑1 (MQ=255)
                                 tGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTgg                                                                                                      >  1:1056387/1‑140 (MQ=255)
                                  gCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGt                                                                                                     >  2:225465/1‑140 (MQ=255)
                                   ccGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGtt                                                                                                    >  1:1188702/1‑140 (MQ=255)
                                    cGCTGACATCGCCGATCATACTGCTGATCGCCATCGGGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTa                                                                                                   <  2:360173/140‑1 (MQ=255)
                                    cGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTa                                                                                                   <  2:446023/140‑1 (MQ=255)
                                      cTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATg                                                                                                 <  1:731117/140‑1 (MQ=255)
                                                   tCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCTCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTgcg                                                                                    >  1:813820/1‑140 (MQ=255)
                                                     aTACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCt                                                                                  <  2:779679/140‑1 (MQ=255)
                                                     aTACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCt                                                                                  >  2:1162230/1‑140 (MQ=255)
                                                       aCTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCa                                                                                <  2:929724/140‑1 (MQ=255)
                                                        ctgctgATCGCCATCGAGATGGTTTCATAACNCCCATCCACCAGCCATGCCANCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCaa                                                                               >  1:391693/1‑140 (MQ=255)
                                                          gctgATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAAt                                                                             >  2:837657/1‑140 (MQ=255)
                                                          gctgATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAAt                                                                             <  1:1213296/140‑1 (MQ=255)
                                                             gATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCg                                                                          >  1:504037/1‑140 (MQ=255)
                                                             gATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCg                                                                          >  2:1178430/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:1278900/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         <  2:1252570/140‑1 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:561309/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:472153/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  1:844420/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  1:1302303/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  1:160263/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:719438/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:647822/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  1:520392/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  1:498759/1‑140 (MQ=255)
                                                              aTCGCCATCGAGATGGGTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGACGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGa                                                                         >  2:1361010/1‑140 (MQ=255)
                                                                cGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATTCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGaaa                                                                       >  2:411231/1‑140 (MQ=255)
                                                                cGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGaaa                                                                       >  1:284013/1‑140 (MQ=255)
                                                                     tCGAGATGGTTTCATAACGCCCATCCACCAGCCATTCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATc                                                                  <  1:411231/140‑1 (MQ=255)
                                                                      cGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCa                                                                 >  2:1364875/1‑140 (MQ=255)
                                                                           tGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCa                                                            >  1:544770/1‑140 (MQ=255)
                                                                                 cATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGcc                                                      <  1:352233/140‑1 (MQ=255)
                                                                                    aaCGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGc                                                   >  2:525354/1‑140 (MQ=255)
                                                                                    aaCGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGc                                                   <  2:83524/140‑1 (MQ=255)
                                                                                       gCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGt                                                >  2:1329095/1‑140 (MQ=255)
                                                                                       gCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGt                                                >  1:708593/1‑140 (MQ=255)
                                                                                        cccATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGtt                                               >  2:303037/1‑140 (MQ=255)
                                                                                            tCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTtcatc                                           >  1:198717/1‑140 (MQ=255)
                                                                                              caccaGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTcatcat                                         <  2:198717/140‑1 (MQ=255)
                                                                                                 caGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCg                                      <  1:1137464/140‑1 (MQ=255)
                                                                                                   gccatgccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCt                                    <  1:719438/140‑1 (MQ=255)
                                                                                                   gccatgccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCt                                    >  1:297699/1‑140 (MQ=255)
                                                                                                    ccatgccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTc                                   >  1:967093/1‑140 (MQ=255)
                                                                                                       tgccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGa                                >  1:1068596/1‑140 (MQ=255)
                                                                                                       tgccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGCTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGa                                >  2:607200/1‑140 (MQ=255)
                                                                                                        gccatCCCGGCGGCGGCCCCCATTGCTGCGGTCGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGaa                               >  2:947660/1‑140 (MQ=255)
                                                                                                        gccatCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGaa                               >  1:1288158/1‑140 (MQ=255)
                                                                                                           atCCCGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAgtg                            <  2:523699/140‑1 (MQ=255)
                                                                                                             cccGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGtt                          >  1:292479/1‑140 (MQ=255)
                                                                                                             cccGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGtt                          <  1:1099117/140‑1 (MQ=255)
                                                                                                             cccGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGtt                          >  1:916619/1‑140 (MQ=255)
                                                                                                             cccGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGtt                          <  1:879774/140‑1 (MQ=255)
                                                                                                             cccGGCGGCGGCCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGtt                          <  1:377761/140‑1 (MQ=255)
                                                                                                                ggcggcggcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTg                       <  2:160263/140‑1 (MQ=255)
                                                                                                                 gcggcggcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTgt                      >  2:457556/1‑139 (MQ=255)
                                                                                                                      ggcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTacca                 <  1:345628/140‑1 (MQ=255)
                                                                                                                      ggcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTacca                 <  1:568291/140‑1 (MQ=255)
                                                                                                                      ggcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTacca                 <  1:225465/140‑1 (MQ=255)
                                                                                                                       gcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTaccac                <  2:391693/140‑1 (MQ=255)
                                                                                                                       gcCCCCATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCACACAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTaccac                <  2:1188702/140‑1 (MQ=255)
                                                                                                                           ccATTGCTGCGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTaccaccacc            <  2:813820/140‑1 (MQ=255)
                                                                                                                             aTTGCTGCGGTAGTTGCGGCACACAGCGAAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTaccaccacccc          <  1:32400/140‑3 (MQ=255)
                                                                                                                                gctgcGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAgg       <  1:1047080/140‑1 (MQ=255)
                                                                                                                                gctgcGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAgg       <  2:284013/140‑1 (MQ=255)
                                                                                                                                gctgcGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAgg       <  2:292479/140‑1 (MQ=255)
                                                                                                                                  tgcGGTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAGGCa     <  2:504037/140‑1 (MQ=255)
                                                                                                                                     ggTAGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAGGCAttg  >  1:444853/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TCGCGCAGCTGTTCGACGCACCATCGCAAATCATGCCGCTGACATCGCCGATCATACTGCTGATCGCCATCGAGATGGTTTCATAACGCCCATCCACCAGCCATGCCATCCCGGCGGCGGCCCCCATTGCTGCGGTCGTTGCGGCACACAGCGCAGACAAACGCGGTAACTGGTTATGGATGTAAATTGCGCTCAAATGCGAAAGCATCAGCGCACGCGCCAGCCGTTCATCATCCGCTCCGAAGTGTTCTGCTACCACCACCACAGGCATTG  >  NC_000913/3255538‑3255810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A