Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F48 I1 R1 83 81.6 2760494 99.1% 2735649 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,936,304 G→T L9L (CTG→CTT)  rbsB → ribose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,3040GT100.0% 262.4 / NA 75L9L (CTG→CTT) rbsBribose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/37);  total (38/37)

TAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAC  >  NC_000913/3936171‑3936440
                                                                                                                                     |                                                                                                                                        
tAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAACAGGAATAACGACTACAGGACATCTTTAATATGAAAATGAAAAAAATGGCTACCCTTGTTTcc                                                                                                                                    >  2:546220/1‑140 (MQ=255)
 aGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCg                                                                                                                                   <  2:1117288/140‑1 (MQ=255)
        tcctccTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTgc                                                                                                                            >  1:366953/1‑140 (MQ=255)
             ctATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTaa                                                                                                                       <  1:523552/140‑1 (MQ=255)
                ttACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAgcg                                                                                                                    >  1:1203963/1‑140 (MQ=255)
                  aCCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTTGTAGACAACAAAAAGCAGGAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGcc                                                                                                                  >  1:727379/1‑140 (MQ=255)
                  aCCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGcc                                                                                                                  >  1:364484/1‑140 (MQ=255)
                      gatgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCg                                                                                                              >  1:311606/1‑140 (MQ=255)
                       atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTTCGCTAAGCGCCACCGt                                                                                                             >  1:429373/1‑140 (MQ=255)
                       atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                             >  1:368122/1‑140 (MQ=255)
                       atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                             >  1:969633/1‑140 (MQ=255)
                        tgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTc                                                                                                            <  2:131437/140‑1 (MQ=255)
                          atCGTCAAAGCGGTGGTGATTTTGCTGGCGGTTCTGGGAGACAACAAAAAACAGTAATAAAGACTACAGGACATCTTTAATATGAACATTAAAAAAAAGGCTACCCTTGTTTCCGCTGTTTTGCTAAACGCCACCCTCAg                                                                                                          >  2:1017515/1‑140 (MQ=255)
                          atCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTTGACAACAAAAAACAGTAATAATGACTTCATGGCATTTTGTATATGAAAATGAAAAAAATGGCTACCCTTTTTTTCCCTTTTTCGCTTAACGCCACCGTCat                                                                                                          >  2:1301005/1‑139 (MQ=255)
                          atCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAg                                                                                                          <  1:1301005/140‑1 (MQ=255)
                            cGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTg                                                                                                        >  1:725335/1‑140 (MQ=255)
                            cGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTTAATATGAACATGAAAAAAATGGCTACCCCTTTTTCCGCTGTTGCGCTAAACGCCCCCGTCAggg                                                                                                        >  1:175344/1‑138 (MQ=255)
                            cGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAACAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTg                                                                                                        >  2:72340/1‑140 (MQ=255)
                            cGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAACAGTAATAAAGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTg                                                                                                        >  2:316587/1‑140 (MQ=255)
                            cGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAACAGGAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTTTTTCCGCTGTTTCGCTAAACGCCACCCTCAGtt                                                                                                        >  2:70218/1‑139 (MQ=255)
                               cAAAGCGGTGGTGATTTTGCTGGCGGCGCCGGGAGACGACAAAAAGCAGTAATAACGACTACAGGACATCTTTAATATGAAAATGAAAAAAATGGCTACCCTTTTTTTCGCTGTTTCGCCAAGCGCCACCGGCAATGCGa                                                                                                     >  2:860778/1‑140 (MQ=255)
                                aaaGCGGTGGTGATTTTGCTGGCGGTGCTGGGAGACAACAAAAAACAGGAATAAAGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGaa                                                                                                    >  2:791343/1‑140 (MQ=255)
                                  aGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATg                                                                                                  >  1:1061969/1‑140 (MQ=255)
                                   gCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGc                                                                                                 <  1:1053331/140‑1 (MQ=255)
                                     ggtggtGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAGCATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGa                                                                                               <  2:188497/140‑1 (MQ=255)
                                     ggtggtGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGa                                                                                               <  2:364484/140‑1 (MQ=255)
                                     ggtggtGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGa                                                                                               <  2:368122/140‑1 (MQ=255)
                                     ggtggtGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGa                                                                                               <  1:1233328/140‑1 (MQ=255)
                                       tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAAAGACTACAGGACATCTTTAATATGAACATGAAAAAACTGGCTACCCTTTTTTCCGCTGTTTCGCTAAGCGCCACCGTCAGTGCGAATGCGAtt                                                                                             >  2:115170/1‑139 (MQ=255)
                                       tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAACAGGAATAACGACTACAGGACATCTTGGATATGAAAAATAAAAAAATGGCTACCCTTGTTTCCGCTGTTTCGCTAAGCGCCACCCTCAGTGCGAATGCGAtt                                                                                             >  2:1058364/1‑139 (MQ=255)
                                       tggtgATTTTGCTGGCGGTGCTGGGAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                             >  1:1332961/1‑140 (MQ=255)
                                        ggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATgg                                                                                            <  2:725335/140‑1 (MQ=255)
                                          tgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCa                                                                                          <  2:366953/140‑1 (MQ=255)
                                                 gCTGGCGGTGCTGGGAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGaca                                                                                   >  1:623051/1‑140 (MQ=255)
                                                      cGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATc                                                                              >  1:915483/1‑140 (MQ=255)
                                                       ggTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcg                                                                             <  1:325589/140‑1 (MQ=255)
                                                       ggTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcg                                                                             <  1:1151847/140‑1 (MQ=255)
                                                                   caacaaAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGtct                                                                 <  2:1332961/140‑1 (MQ=255)
                                                                   caacaaAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGtct                                                                 >  1:990113/1‑140 (MQ=255)
                                                                   caacaaAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGtct                                                                 >  2:607508/1‑140 (MQ=255)
                                                                   aaacaaAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGtct                                                                 <  2:1061969/139‑1 (MQ=255)
                                                                     acaaAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTcc                                                               >  1:682206/1‑140 (MQ=255)
                                                                       aaaaaGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCAc                                                             <  2:692940/140‑1 (MQ=255)
                                                                       aaaaaGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCAc                                                             <  1:1368685/140‑1 (MQ=255)
                                                                            gCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTa                                                        <  1:246482/140‑1 (MQ=255)
                                                                                taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                    >  2:1346097/1‑140 (MQ=255)
                                                                                taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                    >  2:513253/1‑140 (MQ=255)
                                                                                  ataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAAcc                                                  <  2:727379/140‑1 (MQ=255)
                                                                                   taaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAAccc                                                 <  2:1203963/140‑1 (MQ=255)
                                                                                   taaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAAccc                                                 <  2:1319216/140‑1 (MQ=255)
                                                                                   taaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAAccc                                                 <  2:644836/140‑1 (MQ=255)
                                                                                      cGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTACACGCTTAACAACCCGtt                                              <  2:915483/140‑1 (MQ=255)
                                                                                        aCTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCt                                            <  1:702315/140‑1 (MQ=255)
                                                                                          tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCttt                                          >  1:1213301/1‑140 (MQ=255)
                                                                                          tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCttt                                          >  1:287157/1‑140 (MQ=255)
                                                                                            cAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGt                                        >  2:1120479/1‑140 (MQ=255)
                                                                                            cAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGt                                        >  2:491150/1‑140 (MQ=255)
                                                                                            cAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGt                                        >  2:1126891/1‑140 (MQ=255)
                                                                                            cAGGACATCTTGAATATGAACATG‑AAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                       >  2:203102/1‑140 (MQ=255)
                                                                                             aGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                       <  1:791343/140‑1 (MQ=255)
                                                                                             aGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                       <  2:158193/140‑1 (MQ=255)
                                                                                              ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                      <  2:519541/140‑1 (MQ=255)
                                                                                              ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                      <  1:316587/140‑1 (MQ=255)
                                                                                              ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                      <  1:72340/140‑1 (MQ=255)
                                                                                              ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                      <  2:1157572/140‑1 (MQ=255)
                                                                                                 cATCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTATTCGCCACCGTCAGTGCGGATGCGATGGCAAAAGGCACTATCGCGCTGGTGGTCTCTACGCTTAACAACCCGTTCTTTGTATCGc                                   >  2:1298633/1‑140 (MQ=255)
                                                                                                  aTCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCt                                  >  2:600026/1‑140 (MQ=255)
                                                                                                  aTCTTGAATATGAACATGAAAAAACTGGCTACCCATGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCt                                  >  1:420853/1‑140 (MQ=255)
                                                                                                   tCTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTg                                 >  2:64881/1‑140 (MQ=255)
                                                                                                    cTTGAATATGTACATGAAAAAACTGGCTACCCTTGTTTCTGCTGTTGCGCTAAGCGACATCGTCAGTGCGAATGCGAAGGCAAAAGACACCATCGCGCTGTATGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                >  2:325330/1‑140 (MQ=255)
                                                                                                    cTTGAATATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                >  2:361374/1‑140 (MQ=255)
                                                                                                        aaTATGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGa                            <  2:1172581/140‑1 (MQ=255)
                                                                                                            tGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgc                        <  2:287157/140‑1 (MQ=255)
                                                                                                            tGAACATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgc                        >  1:1237640/1‑140 (MQ=255)
                                                                                                              aaCATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgc                      <  2:311606/140‑1 (MQ=255)
                                                                                                               aCATGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                     >  2:959518/1‑140 (MQ=255)
                                                                                                                  tGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAg                  >  1:235535/1‑140 (MQ=255)
                                                                                                                  tGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAg                  >  1:965504/1‑140 (MQ=255)
                                                                                                                  tGAAAAAACTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAg                  >  2:731831/1‑140 (MQ=255)
                                                                                                                        aaCTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag            <  1:1017515/140‑1 (MQ=255)
                                                                                                                        aaCTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag            <  1:1006766/140‑1 (MQ=255)
                                                                                                                         aCTGGCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAgg           <  2:165787/140‑1 (MQ=255)
                                                                                                                           tGGCTACCCTTTTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCg         >  2:269385/1‑140 (MQ=255)
                                                                                                                             gCTACCCTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa       >  2:1180963/1‑140 (MQ=255)
                                                                                                                                  ccTTGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc  <  1:800136/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAC  >  NC_000913/3936171‑3936440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A