Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,872	C→A	Q199K (CAG→AAG)	rbsB →	ribose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,872	0	C	A	100.0%	190.7 / NA	54	Q199K (CAG→AAG)	rbsB	ribose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/27); total (27/27)

AGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTACACCGGA  >  NC_000913/3936735‑3937008

aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAg                                                                                                                                        <  1:1113886/140‑1 (MQ=255)

aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAg                                                                                                                                        <  2:56740/140‑1 (MQ=255)

aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAg                                                                                                                                        <  1:1376135/140‑1 (MQ=255)

aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAg                                                                                                                                        <  1:914910/140‑1 (MQ=255)

  cTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATgaagaa                                                                                                                                      <  2:21165/140‑1 (MQ=255)

  cTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATgaagaa                                                                                                                                      >  1:864376/1‑140 (MQ=255)

     cAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCt                                                                                                                                   >  1:1085662/1‑140 (MQ=255)

             tGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCg                                                                                                                           >  1:360620/1‑140 (MQ=255)

                     aaTCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCg                                                                                                                   <  2:825976/139‑1 (MQ=255)

                      aTCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCgg                                                                                                                  <  2:222463/140‑1 (MQ=255)

                         cGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATg                                                                                                               >  1:1046231/1‑140 (MQ=255)

                           cAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGtt                                                                                                             <  2:719396/140‑1 (MQ=255)

                            aGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTc                                                                                                            >  2:968540/1‑140 (MQ=255)

                            aGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTc                                                                                                            >  2:943821/1‑140 (MQ=255)

                             gCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCa                                                                                                           >  2:371723/1‑140 (MQ=255)

                             gCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCa                                                                                                           >  1:697380/1‑140 (MQ=255)

                                cGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGc                                                                                                        >  2:593247/1‑140 (MQ=255)

                                cGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGc                                                                                                        >  2:1339116/1‑140 (MQ=255)

                                  tGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTTTTGACCGCTCATCCGGATGTTCAGGCtt                                                                                                      >  1:202207/1‑139 (MQ=255)

                                    aaCGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTa                                                                                                    <  1:677104/140‑1 (MQ=255)

                                     aCGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTAt                                                                                                   <  1:63316/140‑1 (MQ=255)

                                          gCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                              <  1:617019/140‑1 (MQ=255)

                                            gAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCa                                                                                            <  1:453670/140‑1 (MQ=255)

                                            gAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCa                                                                                            <  1:631957/140‑1 (MQ=255)

                                             aaGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAg                                                                                           <  2:834088/140‑1 (MQ=255)

                                                   tCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAatgat                                                                                     >  1:311907/1‑140 (MQ=255)

                                                             cGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGgcgc                                                                           <  2:1217379/140‑1 (MQ=255)

                                                                     ctCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                                   >  2:49402/1‑140 (MQ=255)

                                                                           aGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgc                                                             <  1:449372/140‑1 (MQ=255)

                                                                           aGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgc                                                             <  1:578312/140‑1 (MQ=255)

                                                                            gTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTAGTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcg                                                            <  2:356030/140‑1 (MQ=255)

                                                                               tAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCAc                                                         >  2:335412/1‑140 (MQ=255)

                                                                               tAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAAAGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGAGCGCTGCGCGCAc                                                         <  2:202207/140‑1 (MQ=255)

                                                                                      tttGCCATCCAGCCAGCAGATTTTGATCGCATTAACGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                                                  <  2:1077418/139‑1 (MQ=255)

                                                                                      cTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                                                  >  1:527390/1‑140 (MQ=255)

                                                                                        tGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTg                                                >  1:1363508/1‑140 (MQ=255)

                                                                                          ccagccagccagCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                                              <  1:869949/140‑1 (MQ=255)

                                                                                            agccagccagcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                                            <  1:515756/140‑1 (MQ=255)

                                                                                              ccagccagcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTa                                          >  2:190076/1‑140 (MQ=255)

                                                                                                                tCGCATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgtcg                        <  1:855471/140‑1 (MQ=255)

                                                                                                                   cATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg                     >  1:1006868/1‑140 (MQ=255)

                                                                                                                   cATTAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg                     >  1:550442/1‑140 (MQ=255)

                                                                                                                     ttAAAGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGa                   <  2:697380/140‑1 (MQ=255)

                                                                                                                       aaaGGTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGAtt                 <  2:448250/140‑1 (MQ=255)

                                                                                                                          ggTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa              <  1:593247/140‑1 (MQ=255)

                                                                                                                          ggTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa              <  2:1084231/140‑1 (MQ=255)

                                                                                                                          ggTTTGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa              <  2:471212/140‑1 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  1:641819/1‑140 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  1:402994/1‑140 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  1:1121234/1‑140 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  2:398078/1‑140 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  2:288011/1‑140 (MQ=255)

                                                                                                                              tGAACGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt          >  2:1070699/1‑140 (MQ=255)

                                                                                                                                aaCGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTac        >  1:1335994/1‑140 (MQ=255)

                                                                                                                                aaCGTAATGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTac        >  1:142037/1‑140 (MQ=255)

                                                                                                                                     aaTGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTACACCgg   >  1:308396/1‑140 (MQ=255)

                                                                                                                                      aTGAAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTACACCGGa  <  2:360620/140‑1 (MQ=255)

AGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGTACACCGGA  >  NC_000913/3936735‑3937008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A