Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F48 I1 R1 83 81.6 2760494 99.1% 2735649 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,219,183 G→C V196V (GTG→GTC)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,219,1830GC98.8% 322.3 / ‑3.4 85V196V (GTG→GTC) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (43/41);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

TCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACG  >  NC_000913/4219048‑4219318
                                                                                                                                       |                                                                                                                                       
tctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGcga                                                                                                                                     <  1:749886/140‑1 (MQ=255)
 ctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGcgaa                                                                                                                                    <  2:374927/140‑1 (MQ=255)
 ctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGcgaa                                                                                                                                    <  2:578838/140‑1 (MQ=255)
    cTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTTCAGGTCGcgaacga                                                                                                                                 >  1:1307602/1‑140 (MQ=255)
     tGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGcgaacgaa                                                                                                                                <  1:673406/140‑1 (MQ=255)
      gATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCgaacgaac                                                                                                                               >  2:291565/1‑140 (MQ=255)
         gcgcGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGt                                                                                                                            >  2:309244/1‑140 (MQ=255)
         gcgcGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGt                                                                                                                            >  1:916739/1‑140 (MQ=255)
          cgcgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGtt                                                                                                                           >  1:513812/1‑140 (MQ=255)
          cgcgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGtt                                                                                                                           >  2:871559/1‑140 (MQ=255)
           gcgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGttt                                                                                                                          <  2:394390/140‑1 (MQ=255)
            cgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGtttt                                                                                                                         >  2:696022/1‑140 (MQ=255)
            cgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGtttt                                                                                                                         >  2:170117/1‑140 (MQ=255)
              ttATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTAc                                                                                                                       <  1:927623/140‑1 (MQ=255)
                   aGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCaa                                                                                                                  >  2:407199/1‑140 (MQ=255)
                       aCCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATaaa                                                                                                              <  2:165029/140‑1 (MQ=255)
                       aCCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATaaa                                                                                                              <  2:55017/140‑1 (MQ=255)
                           aCCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAgccg                                                                                                          >  2:1162638/1‑140 (MQ=255)
                             cGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAgccgcc                                                                                                        <  1:170117/140‑1 (MQ=255)
                                  cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggct                                                                                                   <  2:1287974/140‑1 (MQ=255)
                                  cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggct                                                                                                   >  2:1017936/1‑140 (MQ=255)
                                  cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggct                                                                                                   >  1:952015/1‑140 (MQ=255)
                                  cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggct                                                                                                   >  2:314682/1‑140 (MQ=255)
                                  cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggct                                                                                                   >  2:789809/1‑140 (MQ=255)
                                   gcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggctg                                                                                                  <  2:657442/140‑1 (MQ=255)
                                   gcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggctg                                                                                                  >  2:220619/1‑140 (MQ=255)
                                    cctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggctgg                                                                                                 >  2:821831/1‑140 (MQ=255)
                                    cctgcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggctgg                                                                                                 <  1:1292845/140‑1 (MQ=255)
                                       gcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAg                                                                                              <  1:696022/140‑1 (MQ=255)
                                           tGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaa                                                                                          <  2:1194256/140‑1 (MQ=255)
                                            ggCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaaa                                                                                         <  1:871559/140‑1 (MQ=255)
                                            ggCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaaa                                                                                         <  2:916739/140‑1 (MQ=255)
                                             gCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAAc                                                                                        >  2:1355066/1‑140 (MQ=255)
                                                 aaTAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGAt                                                                                    >  2:10799/1‑140 (MQ=255)
                                                     aaaGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATcaca                                                                                <  1:1016569/140‑1 (MQ=255)
                                                     aaaGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATcaca                                                                                <  1:921797/140‑1 (MQ=255)
                                                       atccGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACAcc                                                                              >  1:618183/3‑140 (MQ=255)
                                                           gTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTcc                                                                          <  1:1355066/140‑1 (MQ=255)
                                                           gTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTcc                                                                          >  2:87724/1‑140 (MQ=255)
                                                            tGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCg                                                                         >  1:880779/1‑140 (MQ=255)
                                                             gACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCgg                                                                        >  2:491632/1‑140 (MQ=255)
                                                                  ccATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACAt                                                                   >  2:142221/1‑140 (MQ=255)
                                                                      tacattaTTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGcc                                                               >  1:979359/1‑140 (MQ=255)
                                                                          ttattCGCCATCTGACGGAAACGCTTGGGACAGACAACCTCGCGGAAAGTCATTTACAGGACGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttt                                                           >  2:635951/1‑140 (MQ=255)
                                                                           tattCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtttt                                                          >  1:441740/1‑140 (MQ=255)
                                                                           tattCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtttt                                                          >  1:226855/1‑140 (MQ=255)
                                                                            attCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttt                                                         <  1:491632/140‑1 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtttttt                                                        >  2:562535/1‑139 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtttttt                                                        >  2:11912/1‑139 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:152172/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:1193316/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:1037407/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  2:1368538/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  2:1355746/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:396010/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:988900/1‑140 (MQ=255)
                                                                             ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                        >  1:319601/1‑140 (MQ=255)
                                                                              tCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtgc                                                       <  1:361017/140‑1 (MQ=255)
                                                                               cGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtgct                                                      <  1:1162638/140‑1 (MQ=255)
                                                                                 ccATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtgctgc                                                    <  1:220619/140‑1 (MQ=255)
                                                                                 ccATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtgctgc                                                    <  1:553396/140‑1 (MQ=255)
                                                                                  cATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGcc                                                   >  1:635721/1‑140 (MQ=255)
                                                                                  cATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGcc                                                   >  2:399065/1‑140 (MQ=255)
                                                                                  cATCTAACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtgctgca                                                   >  1:17749/1‑139 (MQ=255)
                                                                                   aTCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTTCGGCACATTGCCATTTTTGCTGCCg                                                  <  1:4640/140‑1 (MQ=255)
                                                                                       gACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATcc                                              <  1:309244/140‑1 (MQ=255)
                                                                                        aCGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATcca                                             <  1:10799/140‑1 (MQ=255)
                                                                                         cGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATccac                                            <  2:319601/140‑1 (MQ=255)
                                                                                            aaaCGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAg                                         <  1:1056580/140‑1 (MQ=255)
                                                                                             aaCGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAga                                        <  1:142221/140‑1 (MQ=255)
                                                                                                   ggggACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAgacggacg                                  <  1:1017936/140‑1 (MQ=255)
                                                                                                     ggACAGACAACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGgacgac                                <  1:291565/140‑1 (MQ=255)
                                                                                                      gacagacaACCTCGCGGAAAGTCATTTACAGGGCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGacgacg                               >  1:110386/1‑140 (MQ=255)
                                                                                                       acagacaACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTG‑TTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATAACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGc                             <  1:935260/140‑1 (MQ=255)
                                                                                                          gacaACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGa                           <  2:333735/140‑1 (MQ=255)
                                                                                                           acaACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAg                          >  1:896925/1‑140 (MQ=255)
                                                                                                           acaACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAg                          <  1:821831/140‑1 (MQ=255)
                                                                                                           acaACCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCAGATCCACCAGACGGACGACGGCGAg                          <  2:17749/140‑1 (MQ=255)
                                                                                                              aCCTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGtta                       <  2:979359/140‑1 (MQ=255)
                                                                                                               acTCGCGGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                      <  2:1042451/139‑1 (MQ=255)
                                                                                                                cTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatt                     >  1:260094/1‑140 (MQ=255)
                                                                                                                   gcgGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                  <  2:441740/140‑1 (MQ=255)
                                                                                                                   gcgGAAAGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                  >  2:1059901/1‑140 (MQ=255)
                                                                                                                         aGTCATTTACAGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGCTCCACCAGACGGACGACGGCGAGTTATTTATTGATAc            <  1:635951/140‑1 (MQ=255)
                                                                                                                                   aGGTCGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacg  <  2:152172/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACG  >  NC_000913/4219048‑4219318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A