Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F48 I1 R1	55	131.7	4456318	99.2%	4420667	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,594	C→A	intergenic (‑223/‑170)	acs ← / → nrfA	acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,594	0	C	A	100.0%	591.7 / NA	163	intergenic (‑223/‑170)	acs/nrfA	acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (64/99); total (64/99)

Only 100 of 165 total aligned reads displayed.

CGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGC  >  NC_000913/4287457‑4287728

cGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACtata                                                                                                                                       <  1:2096754/140‑1 (MQ=255)

  aCAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATaaa                                                                                                                                     >  2:809478/1‑140 (MQ=255)

  aCAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATaaa                                                                                                                                     >  1:1686112/1‑140 (MQ=255)

  aCAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATaaa                                                                                                                                     >  2:1694806/1‑140 (MQ=255)

   cAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAg                                                                                                                                    <  1:1516119/140‑1 (MQ=255)

   cAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAg                                                                                                                                    <  2:1040834/140‑1 (MQ=255)

   cAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAg                                                                                                                                    <  1:622848/140‑1 (MQ=255)

     gATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTg                                                                                                                                  <  1:1338500/140‑1 (MQ=255)

     gATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTg                                                                                                                                  <  2:1631298/140‑1 (MQ=255)

      aTCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGGCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTgg                                                                                                                                 >  1:165089/1‑140 (MQ=255)

           gCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTAttt                                                                                                                            <  2:2123411/140‑1 (MQ=255)

             aaaaGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTa                                                                                                                          >  1:922795/1‑140 (MQ=255)

             aaaaGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTa                                                                                                                          <  2:108838/140‑1 (MQ=255)

               aaGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGTAACATGCCGTTATGCATGCTATTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGAATTTTACa                                                                                                                        <  2:1534526/140‑1 (MQ=255)

               aaGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACa                                                                                                                        <  1:1946875/140‑1 (MQ=255)

                aGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACAt                                                                                                                       >  2:299483/1‑140 (MQ=255)

                aGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACAt                                                                                                                       <  1:1365448/140‑1 (MQ=255)

                aGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACAt                                                                                                                       >  2:1534851/1‑140 (MQ=255)

                aGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACAt                                                                                                                       <  1:1694806/140‑1 (MQ=255)

                 gTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATg                                                                                                                      <  1:1029901/140‑1 (MQ=255)

                         gtgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACa                                                                                                              <  1:986673/140‑1 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTAt                                                                                                                              <  1:1829304/123‑1 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTAt                                                                                                                              >  2:1829304/1‑123 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTCCATGCACTTACaa                                                                                                             >  1:955240/1‑140 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACa                                                                                                                        <  1:1105803/129‑1 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACa                                                                                                                        >  2:1105803/1‑129 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACaa                                                                                                             >  1:340188/1‑140 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACaa                                                                                                             >  1:651712/1‑140 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACaa                                                                                                             >  2:12674/1‑140 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACaa                                                                                                             >  2:1993356/1‑140 (MQ=255)

                          tgCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGCGGTATTTTACATGCACTTACaa                                                                                                             >  1:1859507/1‑140 (MQ=255)

                           gCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCTTGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAt                                                                                                            <  2:1731009/140‑1 (MQ=255)

                           gCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAt                                                                                                            >  1:1396406/1‑140 (MQ=255)

                           gCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAt                                                                                                            >  1:85695/1‑140 (MQ=255)

                           gCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAt                                                                                                            <  2:346153/140‑1 (MQ=255)

                            cAAATAAACGGCAGGGTAATTTTTGATGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAtt                                                                                                           >  1:540351/1‑140 (MQ=255)

                            cAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAAtt                                                                                                           >  1:1010114/1‑140 (MQ=255)

                              aaTAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGa                                                                                                         <  1:299483/140‑1 (MQ=255)

                               aTAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGAt                                                                                                        >  2:1197086/1‑140 (MQ=255)

                                  aaCGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTaa                                                                                                     <  2:84921/140‑1 (MQ=255)

                                    cGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAg                                                                                                   >  2:175526/1‑140 (MQ=255)

                                    cGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAg                                                                                                   >  1:1340888/1‑140 (MQ=255)

                                    cGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAg                                                                                                   <  1:1256454/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  1:1534851/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  2:2053377/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  2:1375820/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  2:671943/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  2:639966/140‑1 (MQ=255)

                                      gCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGac                                                                                                 <  2:507633/140‑1 (MQ=255)

                                       cAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGaca                                                                                                >  2:1464171/1‑140 (MQ=255)

                                        aGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGacaa                                                                                               <  2:922795/140‑1 (MQ=255)

                                        aGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGacaa                                                                                               <  1:2188525/140‑1 (MQ=255)

                                         gggTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGacaac                                                                                              <  2:1010114/140‑1 (MQ=255)

                                          ggTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGacaaca                                                                                             >  2:1658699/1‑140 (MQ=255)

                                           gTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACa                                                                                                              <  1:1303542/122‑1 (MQ=255)

                                           gTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACAt                                                                                            >  1:1214507/1‑140 (MQ=255)

                                           gTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACAt                                                                                            >  1:1661683/1‑140 (MQ=255)

                                              aTTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTca                                                                                         <  1:1790386/140‑1 (MQ=255)

                                                  ttGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAgt                                                                                     <  1:718103/140‑1 (MQ=255)

                                                    gAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAgtgt                                                                                   >  1:252118/1‑140 (MQ=255)

                                                    gAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAgtgt                                                                                   >  1:403685/1‑140 (MQ=255)

                                                    gAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGCATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAgtgt                                                                                   >  1:181408/1‑140 (MQ=255)

                                                      aGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTgg                                                                                 <  1:2139125/140‑1 (MQ=255)

                                                        gTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTACCTATCAAGAGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGtc                                                                               <  2:2093775/140‑2 (MQ=255)

                                                          cAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAt                                                                             <  1:1635066/140‑1 (MQ=255)

                                                          cAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAt                                                                             <  2:1340888/140‑1 (MQ=255)

                                                          cAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAt                                                                             <  1:2072733/140‑1 (MQ=255)

                                                          cAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAt                                                                             <  2:540351/140‑1 (MQ=255)

                                                          aaGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAt                                                                             <  2:165089/139‑1 (MQ=255)

                                                           aGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACAtt                                                                                           <  1:1317989/125‑1 (MQ=255)

                                                           aGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAAACAACAtt                                                                                           >  2:1317989/1‑125 (MQ=255)

                                                               acaaAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATTCTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTTATTAAAGACAACATTCACAGTGTGGTTATTTGtt                                                                        <  1:246906/140‑1 (MQ=255)

                                                               acaaAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGtt                                                                        <  2:2070502/140‑1 (MQ=255)

                                                               acaaAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGtt                                                                        <  1:2222030/140‑1 (MQ=255)

                                                                     gTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACAt                                                                  <  2:1677232/140‑1 (MQ=255)

                                                                      ttGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCTCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATa                                                                 <  1:135356/140‑1 (MQ=255)

                                                                         aTTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑ggg                                                             <  1:175526/140‑1 (MQ=255)

                                                                          ttAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑gggg                                                            >  1:725496/1‑140 (MQ=255)

                                                                               tCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGc                                                       <  2:251225/140‑1 (MQ=255)

                                                                               tCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGc                                                       <  1:920238/140‑1 (MQ=255)

                                                                                cTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCa                                                      <  2:1250417/140‑1 (MQ=255)

                                                                                  ttGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAAt                                                    >  1:1009714/1‑140 (MQ=255)

                                                                                      ggAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCa                                                <  1:2109839/140‑1 (MQ=255)

                                                                                       gAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTA‑TTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCAtt                                              >  2:345143/1‑139 (MQ=255)

                                                                                         aCATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGa                                             <  2:181408/140‑1 (MQ=255)

                                                                                         aCATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGa                                             <  1:1676243/140‑1 (MQ=255)

                                                                                         aCATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGa                                             <  2:403685/140‑1 (MQ=255)

                                                                                                  tATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAgg                                    <  2:1505770/140‑1 (MQ=255)

                                                                                                  tATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAgg                                    <  1:1999589/140‑1 (MQ=255)

                                                                                                   atgcatgcTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGt                                   >  1:1367662/1‑140 (MQ=255)

                                                                                                         gcTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGt                              >  2:771293/1‑140 (MQ=255)

                                                                                                         gcTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCAt                                               <  1:2202883/122‑1 (MQ=255)

                                                                                                         gcTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCAt                                               >  2:2202883/1‑122 (MQ=255)

                                                                                                           tGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTAc                           >  2:1325857/1‑140 (MQ=255)

                                                                                                           tGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTAc                           >  1:16118/1‑140 (MQ=255)

                                                                                                             ttGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAgtgt                                       >  2:627227/1‑126 (MQ=255)

                                                                                                              tGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGCCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGt                        >  2:250820/1‑140 (MQ=255)

                                                                                                                   aaGAGGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGa                   >  1:1519543/1‑140 (MQ=255)

                                                                                                                     gagGAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGaaa                 >  1:472365/1‑140 (MQ=255)

                                                                                                                        gAAGATACTGACTAACTATAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAAc              >  1:2124599/1‑140 (MQ=255)

CGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATA‑GGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGC  >  NC_000913/4287457‑4287728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A