Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,743	G→T	T326T (ACG→ACT)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,743	0	G	T	100.0%	135.1 / NA	39	T326T (ACG→ACT)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/23); total (16/23)

AAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGG  >  NC_000913/3026610‑3026879

aaaaaGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATc                                                                                                                                    <  2:279315/140‑1 (MQ=255)

   aaGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGt                                                                                                                                 <  2:1090909/140‑1 (MQ=255)

           gATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGaa                                                                                                                          <  2:9322/139‑1 (MQ=255)

           gATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGaa                                                                                                                          >  1:9322/1‑139 (MQ=255)

             tCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCa                                                                                                                       >  2:671427/1‑140 (MQ=255)

                tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACtt                                                                                                                    >  1:313947/1‑140 (MQ=255)

                tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACtt                                                                                                                    >  1:373920/1‑140 (MQ=255)

                       gAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGACAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACAt                                                                                                             <  1:1023468/140‑1 (MQ=255)

                             aaaTCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCa                                                                                                       <  2:585076/140‑1 (MQ=255)

                                        tGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACg                                                                                            <  2:130543/140‑1 (MQ=255)

                                          cTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa                                                                                          <  2:803438/140‑1 (MQ=255)

                                          cTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa                                                                                          <  2:203235/140‑1 (MQ=255)

                                               tGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTa                                                                                     >  1:381351/1‑140 (MQ=255)

                                               tGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTa                                                                                     <  2:1141650/140‑1 (MQ=255)

                                                 tCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa                                                                                   <  2:676278/140‑1 (MQ=255)

                                                 tCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa                                                                                   <  2:407690/140‑1 (MQ=255)

                                                 tCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa                                                                                   <  1:963109/140‑1 (MQ=255)

                                                    gACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACaaat                                                                                >  2:982952/1‑139 (MQ=255)

                                                      ccTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTa                                                                              <  1:103194/140‑1 (MQ=255)

                                                         ccAACCTTTACCTCGGCAGCGGCTTATTCAACTCTAAATAAGCATCGCAGAAGAAAGTGAAAGCGGGCATGGGAACTGATATCGGGGCCGGAACTACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTg                                                                           >  2:1076547/1‑140 (MQ=255)

                                                          cAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGc                                                                          <  2:1032904/140‑1 (MQ=255)

                                                                       ggCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATGTCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAATCCTACAAAGTATTGCAATTACAAGGCTa                                                             >  2:1206405/1‑140 (MQ=255)

                                                                        gCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTAt                                                            <  2:435089/140‑1 (MQ=255)

                                                                           gCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGc                                                         <  2:373920/140‑1 (MQ=255)

                                                                             ggCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCct                                                       <  2:989140/140‑1 (MQ=255)

                                                                                   ttCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGc                                                 >  1:680594/1‑140 (MQ=255)

                                                                                         ttGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGa                                           >  1:424046/1‑140 (MQ=255)

                                                                                             aaaaaGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCg                                       >  2:1109760/1‑140 (MQ=255)

                                                                                                   cATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCAGCATATGAAGCGTTTTAc                                 <  1:1161512/140‑1 (MQ=255)

                                                                                                       gCAGAAGAAAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTgg                             <  1:991271/140‑1 (MQ=255)

                                                                                                             gaaAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGc                       >  1:96151/1‑140 (MQ=255)

                                                                                                             gaaAGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGc                       >  2:1135480/1‑140 (MQ=255)

                                                                                                              aaaGTTAAAGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCt                      <  2:651671/140‑1 (MQ=255)

                                                                                                                     aaGTGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGa               >  1:586332/1‑140 (MQ=255)

                                                                                                                        tGGGCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCg            >  2:165095/1‑140 (MQ=255)

                                                                                                                          ggCATGGGAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGaa          <  1:62529/140‑1 (MQ=255)

                                                                                                                               gggAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAAtctc     >  2:287375/1‑140 (MQ=255)

                                                                                                                                ggAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATctct    <  2:680594/140‑1 (MQ=255)

                                                                                                                                 gAACTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTg   <  1:1135480/140‑1 (MQ=255)

                                                                                                                                  aaCTGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTgg  >  2:1237432/1‑140 (MQ=255)

AAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGG  >  NC_000913/3026610‑3026879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A