Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F48 I1 R1 70 76.3 2572620 98.7% 2539175 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,036,906 G→T R401S (CGC→AGC)  recJ ← ssDNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,036,9060GT97.0% 225.6 / 0.6 66R401S (CGC→AGC) recJssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (38/26);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGC  >  NC_000913/3036772‑3037036
                                                                                                                                      |                                                                                                                                  
aaaaAGTTCTAATTAACCCTCTTCCATCTACAAACCCGACGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCgg                                                                                                                               <  2:136212/140‑1 (MQ=255)
    agTTTGAATTTATCCTCTCCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAAcc                                                                                                                           <  2:231592/140‑1 (MQ=255)
       ttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCttt                                                                                                                        <  1:817864/140‑1 (MQ=255)
        tGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTc                                                                                                                       >  2:927217/1‑140 (MQ=255)
              tATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTa                                                                                                                 >  1:220331/1‑140 (MQ=255)
              tATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTa                                                                                                                 >  1:851478/1‑140 (MQ=255)
                    ctTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCa                                                                                                           >  2:259232/1‑140 (MQ=255)
                    ctTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCT‑ATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCAc                                                                                                          >  1:204697/1‑140 (MQ=255)
                     ttCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCAc                                                                                                          >  1:822218/1‑140 (MQ=255)
                          gCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACctgct                                                                                                     >  1:250048/1‑140 (MQ=255)
                           cGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGATACCGGAACCTTTCAGCGTACCGTCACctgctg                                                                                                    >  1:124862/1‑140 (MQ=255)
                              cAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGgcg                                                                                                 >  2:298247/1‑140 (MQ=255)
                              cAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGgcg                                                                                                 >  2:95050/1‑140 (MQ=255)
                                 aCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                                                                                              >  2:47328/1‑140 (MQ=255)
                                  cccGCCGCCATCGCATGACCGCCAAACTTCAGCTTCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                                                                                             <  1:1081879/140‑1 (MQ=255)
                                       cgccATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGa                                                                                        <  1:228151/140‑1 (MQ=255)
                                          cATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                                                                                     >  1:1247207/1‑140 (MQ=255)
                                              gCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCgg                                                                                 <  2:1115761/140‑1 (MQ=255)
                                                     cGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGa                                                                          <  2:1083202/140‑1 (MQ=255)
                                                       ccAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa                                                                        <  2:752346/140‑1 (MQ=255)
                                                       acAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa                                                                        <  2:374345/139‑1 (MQ=255)
                                                          aaCTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACg                                                                     >  2:1009969/1‑140 (MQ=255)
                                                                aGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt                                                               >  2:1136953/1‑140 (MQ=255)
                                                                       tGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc                                                        >  1:184579/1‑140 (MQ=255)
                                                                       tGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc                                                        >  2:1079114/1‑140 (MQ=255)
                                                                            gggTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGATGGTGAAAACGCTCTTTGATGCGCGAAGc                                                   <  2:1247207/140‑1 (MQ=255)
                                                                            gggTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGc                                                   >  2:622891/1‑140 (MQ=255)
                                                                                 gagTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGaa                                              >  1:770377/1‑140 (MQ=255)
                                                                                  agTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAAt                                             >  1:812982/1‑140 (MQ=255)
                                                                                  agTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAAt                                             >  1:936632/1‑140 (MQ=255)
                                                                                  agTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAAt                                             >  2:926823/1‑140 (MQ=255)
                                                                                  agTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAAt                                             >  2:1201077/1‑140 (MQ=255)
                                                                                   gtgtgtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATa                                            >  1:745524/1‑140 (MQ=255)
                                                                                      tgtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCg                                         >  1:454109/1‑140 (MQ=255)
                                                                                      tgtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCg                                         >  2:920068/1‑140 (MQ=255)
                                                                                      tgtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCg                                         >  1:434580/1‑140 (MQ=255)
                                                                                       gtCTAATCGCTCCAGCGCATCACGCATATGCAGCCTCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAATCCAGAATAccta                                        >  2:542054/1‑138 (MQ=255)
                                                                                       gtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGa                                        >  2:114856/1‑140 (MQ=255)
                                                                                       gtCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGa                                        >  2:1251370/1‑140 (MQ=255)
                                                                                        tCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGAc                                       >  1:396255/1‑140 (MQ=255)
                                                                                         cTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACa                                      >  1:94392/1‑140 (MQ=255)
                                                                                           aaTCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAAc                                    <  1:723633/140‑1 (MQ=255)
                                                                                              cGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGcc                                 <  2:610284/140‑1 (MQ=255)
                                                                                              cGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGcc                                 <  2:454109/140‑1 (MQ=255)
                                                                                               gCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGccc                                <  1:1136953/140‑1 (MQ=255)
                                                                                                cTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt                               <  1:979599/140‑1 (MQ=255)
                                                                                                cTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt                               >  1:680282/1‑140 (MQ=255)
                                                                                                   cAGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGAt                            <  2:1242116/140‑1 (MQ=255)
                                                                                                    aGCGCATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg                           <  2:220331/140‑1 (MQ=255)
                                                                                                      cgcATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATgtc                         >  1:591080/1‑138 (MQ=255)
                                                                                                       gcATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCa                        <  1:259232/140‑1 (MQ=255)
                                                                                                       gcATCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCa                        <  2:936632/140‑1 (MQ=255)
                                                                                                         aTCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAtt                      <  1:927217/140‑1 (MQ=255)
                                                                                                         aTCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAtt                      <  1:604276/140‑1 (MQ=255)
                                                                                                          tCACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACTTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCATAATACCGACAACGCCCTGATGCCATTc                     <  2:253454/140‑1 (MQ=255)
                                                                                                           cACGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCg                    <  2:812982/140‑1 (MQ=255)
                                                                                                             cGCATATGCAGCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCggg                  <  1:978405/140‑1 (MQ=255)
                                                                                                               aatatGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGt                <  2:434580/139‑1 (MQ=255)
                                                                                                                       gCCCCTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt        >  2:955306/1‑140 (MQ=255)
                                                                                                                        ccccTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg       >  2:157451/1‑140 (MQ=255)
                                                                                                                        ccccTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg       >  1:694149/1‑140 (MQ=255)
                                                                                                                        ccccTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg       >  1:346153/1‑140 (MQ=255)
                                                                                                                        ccccTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg       >  1:101154/1‑140 (MQ=255)
                                                                                                                        cccaTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg       <  2:655282/140‑1 (MQ=255)
                                                                                                                           cTGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCa    <  1:1009969/140‑1 (MQ=255)
                                                                                                                            tGAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAg   >  1:1146118/1‑140 (MQ=255)
                                                                                                                             gAATGGAGCTACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGc  <  1:1079114/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGC  >  NC_000913/3036772‑3037036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A